The interaction between MALAT1 target, miR-143-3p, and RALGAPA2 is affected by functional SNP rs3827693 in breast cancer

Dolatabadi, Nasrin Fattahi; Dehghani, Arezo; Shahand, Elham; Yazdanshenas, Mohammadreza; Tabatabaeian, Hossein; Zamani, Atefe; Azadeh, Mansoureh; Ghaedi, Kamran

doi:10.1007/s13577-020-00422-x

Cited by 18 publications

(10 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the majority of proteins are shared identifications between BC patient and healthy control groups (~82%), as well as the databases we used, interestingly, we found 11 proteins which are uniquely identified only from BC patient using NanoPoms preparation (Supplementary Table s3 ). Those proteins have previously been reported to be associated with bladder cancer metastases, including IRAK4 58 , KRT23 59 , and RALGAPA2 60 (full list in Supplementary Table s3 ). Also 4 proteins were found uniquely in the healthy group using NanoPoms preparation, but not in cancer patients.…”

Section: Resultsmentioning

confidence: 99%

Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

Thippabhotla

Zhong

et al. 2022

Commun Biol

View full text Add to dashboard Cite

Extracellular vesicles (EVs), particularly nano-sized small EV exosomes, are emerging biomarker sources. However, due to heterogeneous populations secreted from diverse cell types, mapping exosome multi-omic molecular information specifically to their pathogenesis origin for cancer biomarker identification is still extraordinarily challenging. Herein, we introduced a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome. This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome (Data are available via ProteomeXchange with identifier PXD034454). The NanoPoms prepared exosomes also exhibit distinctive in vivo biodistribution patterns, highlighting the highly viable and integral quality. The developed method is simple and straightforward, which is applicable to nearly all types of biological fluids and amenable for enrichment, scale up, and high-throughput exosome isolation.

show abstract

Section: Resultsmentioning

confidence: 99%

Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

Thippabhotla

Zhong

et al. 2022

Commun Biol

View full text Add to dashboard Cite

show abstract

“…Genetic variation of lncRNA may have an important role in disease susceptibility via affecting lncRNA binding capacity to proteins or miRNA and their loop structure (Guo et al, 2016;Chen et al, 2018;Fattahi Dolatabadi et al, 2020;Feng et al, 2020;Fu et al, 2020). For example, the interaction between MALAT1 target, miR-143-3p, and RALGAPA2 is affected by a functional SNP rs3827693 in breast cancer (Fattahi Dolatabadi et al, 2020). The SNP rs12982687 affects binding capacity of lncRNA UCA1 to miR-873-5p (Fu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

Zhao

Hou

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

lncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (plncMGPF) gene were identified among commercial pig breeds and Chinese local pig breeds. These SNPs are highly linked and constructed into multiple haplotypes, and haplotype ATTCATGTTC (H1) mainly exists in commercial pig breeds while haplotype GCCTGCACCT (H3) is more frequent in Chinese local pig breeds. Association analysis indicated that all SNPs are significantly associated with the backfat thickness and loin muscle area (P < 0.05), respectively, and homologous H1 individuals have higher loin muscle area and lower backfat thickness than H3 pigs. Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. Further experiments showed that two SNPs (rs81403974 and rs325492834) function together to confer plncMGPF stability and function. Our observation suggested that the SNPs in lncMGPF can change the RNA stabilities and lncMGPF function, thereby affecting the porcine meat production traits.

show abstract

“…Such overexpressed MALAT1 can promote BC proliferation and progression by repressing various RNA molecules. For example, it can competitively bind with miR-1 and thereby affect the expression of CDC42 [ 189 ], downregulate hsa-miR-448, lead to aberrant expression of KDM5B [ 190 ], and regulate the expression of miR-143-3p and its putative target, RALGAPA2 [ 191 ]. Huang et al detected the MALAT1 expression levels in tissue samples collected from 20 BC patients and 20 healthy controls and found significantly higher expression levels in the former collective ( p < 0.05).…”

Section: Lncrnas In Female-oriented Cancersmentioning

confidence: 99%

The Role of Long Non-Coding RNAs (lncRNAs) in Female Oriented Cancers

Naz

Tariq

Ali

et al. 2021

Cancers

View full text Add to dashboard Cite

Recent advances in molecular biology have discovered the mysterious role of long non-coding RNAs (lncRNAs) as potential biomarkers for cancer diagnosis and targets for advanced cancer therapy. Studies have shown that lncRNAs take part in the incidence and development of cancers in humans. However, previously they were considered as mere RNA noise or transcription byproducts lacking any biological function. In this article, we present a summary of the progress on ascertaining the biological functions of five lncRNAs (HOTAIR, NEAT1, H19, MALAT1, and MEG3) in female-oriented cancers, including breast and gynecological cancers, with the perspective of carcinogenesis, cancer proliferation, and metastasis. We provide the current state of knowledge from the past five years of the literature to discuss the clinical importance of such lncRNAs as therapeutic targets or early diagnostic biomarkers. We reviewed the consequences, either oncogenic or tumor-suppressing features, of their aberrant expression in female-oriented cancers. We tried to explain the established mechanism by which they regulate cancer proliferation and metastasis by competing with miRNAs and other mechanisms involved via regulating genes and signaling pathways. In addition, we revealed the association between stated lncRNAs and chemo-resistance or radio-resistance and their potential clinical applications and future perspectives.

show abstract

The interaction between MALAT1 target, miR-143-3p, and RALGAPA2 is affected by functional SNP rs3827693 in breast cancer

Cited by 18 publications

References 55 publications

Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

The Role of Long Non-Coding RNAs (lncRNAs) in Female Oriented Cancers

Contact Info

Product

Resources

About