Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

He, Nan; Thippabhotla, Sirisha; Zhong, Cuncong; Greenberg, Zachary; Xu, Liang; Pessetto, Ziyan Y.; Godwin, Andrew K.; Zeng, Yong; He, Mei

doi:10.1038/s42003-022-03598-0

Cited by 20 publications

(17 citation statements)

References 74 publications

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“…Ultrafiltration is subject to EV destruction due to the shear force of membrane filtration. Currently, affinity purification such as immunomagnetic beads and affinity column are getting more recognition in terms of homogeneous population and purity relevant to interests [ 139 ]. Due to variance in EV isolation and variable return of results from widely accepted methods, it is important that highly specific and sensitive isolation techniques are developed in the future for improving diagnostic outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…For developing exosome based diagnostic test, the well-established exosome isolation and characterization are critical and need to be standardized. Some pioneer research work has been reported recently to overcome the isolation challenge and ensure the exosome purity and specificity [ 84 , 96 , 139 ]. However, clinical translation is still lacking.…”

Section: Discussionmentioning

confidence: 99%

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Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

Walker

O’Malley

2022

Pharmaceutics

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Muscle Invasive Bladder Cancer (MIBC) is a subset of bladder cancer with a significant risk for metastases and death. It accounts for nearly 25% of bladder cancer diagnoses. A diagnostic work-up for MIBC is inclusive of urologic evaluation, radiographic imaging with a CT scan, urinalysis, and cystoscopy. These evaluations, especially cystoscopy, are invasive and carry the risk of secondary health concerns. Non-invasive diagnostics such as urine cytology are an attractive alternative currently being investigated to mitigate the requirement for cystoscopy. A pitfall in urine cytology is the lack of available options with high reliability, specificity, and sensitivity to malignant bladder cells. Exosomes are a novel biomarker source which could resolve some of the concerns with urine cytology, due to the high specificity as the surrogates of tumor cells. This review serves to define muscle invasive bladder cancer, current urine cytology methods, the role of exosomes in MIBC, and exosomes application as a diagnostic tool in MIBC. Urinary exosomes as the specific populations of extracellular vesicles could provide additional biomarkers with specificity and sensitivity to bladder malignancies, which are a consistent source of cellular information to direct clinicians for developing treatment strategies. Given its strong presence and differentiation ability between normal and cancerous cells, exosome-based urine cytology is highly promising in providing a perspective of a patient’s bladder cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

Walker

O’Malley

2022

Pharmaceutics

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“…It is well-known that compared to bare beads, 3D nanostructures produce better capture efficiencies. 48 Therefore, we expected that binding kinetics of biomarker molecules with the probes on the MB-ZnO NRs might be improved and thus fluorescence signal can be measured in the diffusion-limit condition. The intensity from the multiple fluorescence images was obtained for each MB-ZnO NRs samples over the period of incubation, and the results are shown in Figure 3e.…”

Section: Biosensing Platform Based Onmentioning

confidence: 99%

Fluorescence-Enhanced Microfluidic Biosensor Platform Based on Magnetic Beads with Highly Stable ZnO Nanorods for Biomarker Detection

Meeseepong

Ghosh

Shrivastava

et al. 2023

ACS Appl. Mater. Interfaces

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Existing affinity-based fluorescence biosensing systems for monitoring of biomarkers often utilize a fixed solid substrate immobilized with capture probes limiting their use in continuous or intermittent biomarker detection. Furthermore, there have been challenges of integrating fluorescence biosensors with a microfluidic chip and low-cost fluorescence detector. Herein, we demonstrated a highly efficient and movable fluorescence-enhanced affinity-based fluorescence biosensing platform that can overcome the current limitations by combining fluorescence enhancement and digital imaging. Fluorescence-enhanced movable magnetic beads (MBs) decorated with zinc oxide nanorods (MB-ZnO NRs) were used for digital fluorescence-imaging-based aptasensing of biomolecules with improved signal-to-noise ratio. High stability and homogeneous dispersion of photostable MB-ZnO NRs were obtained by grafting bilayered silanes onto the ZnO NRs. The ZnO NRs formed on MB significantly improved the fluorescence signal up to 2.35 times compared to the MB without ZnO NRs. Moreover, the integration of a microfluidic device for flow-based biosensing enabled continuous measurements of biomarkers in an electrolytic environment. The results showed that highly stable fluorescence-enhanced MB-ZnO NRs integrated with a microfluidic platform have significant potential for diagnostics, biological assays, and continuous or intermittent biomonitoring.

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“…This label-free technique can directly quantify exosomes in serum samples and has shown diagnostic potential for prostate cancer. Additionally, the porous structure has been used to isolate exosomes with and without other isolation materials and devices [ 191 , 192 , 193 ].…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection

Cited by 20 publications

References 74 publications

Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

Fluorescence-Enhanced Microfluidic Biosensor Platform Based on Magnetic Beads with Highly Stable ZnO Nanorods for Biomarker Detection

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

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