The interaction between human breast cancer resistance protein (BCRP) and five bisbenzylisoquinoline alkaloids

In vitro characterization of ABC transporters involved in the absorption and distribution of liensinine and its analogs

“…Additionally, no marked difference was observed in liensinine uptake by MDCK-MRP2 and MDCK cells regardless of co-incubation with MK-571, suggesting that MRP2 may be not involved in the accumulation of liensinine. Combined with our results of the transcellular transport of liensinine across LLC-PK1/LLC-PK1-BCRP cell monolayers (Tian et al, 2013), it was suggested that both P-gp and BCRP, but not MRP2, participate in the active transport of liensinine, which was confirmed by the ER of >2 in Caco-2 cells in the presence of verapamil and well explained its low permeation through the blood brain barrier.…”

Cyclosporin A affects the bioavailability of ginkgolic acids via inhibition of P-gp and BCRP

“…The MDR1-transfected MDCK-MDR1 cells, BCRP-transfected LLC-PK1-BCRP cells and their mock cells were established in our laboratory [20,21]. MDCK cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS, Gibco, USA).…”

“…However, the cells have to be cultured for more than 20 days before one can conduct experiments. The other cell lines, Madin-Darby canine kidney-multidrug resistance protein 1(MDCK-MDR1) are MDCK cell stably transfected with human P-gp, and LLC-PK1-BCRP is the porcine proximal renal epithelial cell line over-expressing with BCRP [20,21]. The MDCK and LLC-PK1 cells grow and differentiate quickly.…”

“…The MDCK and LLC-PK1 cells grow and differentiate quickly. Thus, these two cell models are good alternative to the Caco-2 cells for the evaluation of interactions between NCEs and substrates or inhibitors of P-gp and BCRP [20,[22][23][24][25].…”

Isotetrandrine Reduces Astrocyte Cytotoxicity in Neuromyelitis Optica by Blocking the Binding of NMO-IgG to Aquaporin 4

“…Several phytochemical studies have revealed that M. japonica contains many bioactive substances, including more than 20 kinds of alkaloids. Among these alkaloid constituents, tetrandrine (1S,1′S-tetrandrine) has been widely studied for antitumor activity [16,17,18], multidrug resistance [19,20], the induction of apoptosis [21] and the protection of mouse retinal ganglion cells [22], etc. Moreover, tetrandrine also has an effect on reducing astrocyte cytotoxicity in NMO [23].…”