In vitro characterization of ABC transporters involved in the absorption and distribution of liensinine and its analogs

Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

“…The identified biologically active substances including liensinine, isoliensinine and neferine ( Fig. 1) are the three major alkaloids isolated from Lotus Plumule (Itoh et al, 2011;Yu et al, 2013). Recently, various studies have indicated that these bisbenzylisoquinoline alkaloids have beneficial pharmacological effects, e.g.…”

“…There have several analytical methods been published for simultaneous determination of liensinine, isoliensinine and neferine in the plants and biological samples, including HPLC (Du et al, 2011;Huang et al, 2011), HSCCC (Liu et al, 2009), LC-MS/ MS (Chen et al, 2007;Zhou et al, 2007;Yu et al, 2013) and LC-TOF/ MS (Lin et al, 2013). However, to our best knowledge, there are only two HPLC bioanalytical methods for simultaneous determination of the three alkaloids in biological fluids, but the methods required elaborate sample preparation, long analytical time, and time-consuming (Du et al, 2011;Huang et al, 2011).…”

Cyclosporin A affects the bioavailability of ginkgolic acids via inhibition of P-gp and BCRP

“…The MDR1-transfected MDCK-MDR1 cells, BCRP-transfected LLC-PK1-BCRP cells and their mock cells were established in our laboratory [20,21]. MDCK cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS, Gibco, USA).…”

“…However, the cells have to be cultured for more than 20 days before one can conduct experiments. The other cell lines, Madin-Darby canine kidney-multidrug resistance protein 1(MDCK-MDR1) are MDCK cell stably transfected with human P-gp, and LLC-PK1-BCRP is the porcine proximal renal epithelial cell line over-expressing with BCRP [20,21]. The MDCK and LLC-PK1 cells grow and differentiate quickly.…”

Quantitative Analysis and Comparison of Flavonoids in Lotus Plumules of Four Representative Lotus Cultivars

“…Alkaloids in lotus plumule are main liensiline, neferine and isoliensiline [12]. Early studies revealed that the biological activities of these alkaloids include antihypertensive, hypoglycemic and anti-human immunodeficiency effects, and so on [13,14].…”