The Integration of Whole-Cell Biosensors for the Field-Ready Electrochemical Detection of Arsenic

Sanchez, Sergio I.; McDonald, Mhairi; Silver, Dylan M.; Bonnault, Sandie de; Chen, Cheng; LeBlanc, Katie; Hicks, Emily C.; Mayall, Robert M.

doi:10.1149/1945-7111/ac049e

Cited by 9 publications

(5 citation statements)

References 42 publications

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“…When integrated within a customised hardware system, the reported whole-cell biosensor demonstrated excellent specificity and sensitivity with an LOD 95% confidence, (FRED-As) of 2.2 ppb As( iii ). 99 This sensitive and easy-to-use approach may be a viable alternative for on-site arsenic testing.…”

Section: Analytical Methods and Techniques For Arsenic Determinationmentioning

confidence: 99%

“…The integration of WCBs for the field-ready electrochemical detection of arsenic (FRED-As) has been reported recently. 99 Sergio Sánchez et al reported a WCB biosensor which was followed by electrochemical measurements and provided enhanced accuracy and signal intensity compared to traditional bacterialdetection approaches (Figure 11). FRED-As had a number of benefits including ease of use, potential for measuring a wide spectrum of metals, sensitivity etc.…”

Section: Whole Cell Biosensors (Wcbs)mentioning

confidence: 99%

“…When integrated within a customised hardware system, the reported whole-cell biosensor demonstrated excellent specificity and sensitivity with an LOD 95% confidence, FRED-As) of 2.2 ppb As(III). 99 This sensitive and easy-to-use approach may be a viable alternative for on-site arsenic testing. in their review article nicely summarised the performance of the fluorescent and colorimetric aptasensors which is presented in Figure 12 and discussed.…”

Section: Whole Cell Biosensors (Wcbs)mentioning

confidence: 99%

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Review of analytical techniques for arsenic detection and determination in drinking water

Bhat

Hara²,

Tian

et al. 2023

Environ. Sci.: Adv.

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Section: Analytical Methods and Techniques For Arsenic Determinationmentioning

confidence: 99%

Section: Whole Cell Biosensors (Wcbs)mentioning

confidence: 99%

Section: Whole Cell Biosensors (Wcbs)mentioning

confidence: 99%

See 1 more Smart Citation

Review of analytical techniques for arsenic detection and determination in drinking water

Bhat

Hara²,

Tian

et al. 2023

Environ. Sci.: Adv.

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show abstract

“…Similarly, the detection of As(III) and Hg(II) in contaminated water was reported using engineered E. coli , where the expression level of the lacZ gene increases in the presence of the target that resulted in the synthesis of the reporter protein, β-galactosidase. There are several other reports of using whole cells-based electrochemical biosensors for the detection of heavy metals and toxins in contaminated water. ,, …”

Section: Biorecognition Elements and Signal Transductionmentioning

confidence: 99%

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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“…Biosensors have been used in wearable medical monitoring, health diagnosis, and environmental monitoring. − Natural evolution of receptors have led to microbes’ ability to detect and respond to known human health hazards, such as phenanthrene, lead, copper, and organophosphate pesticides. , These molecular components provide a basis for sensor engineering in living systems. One environmental pollutant for which there is currently no zero-power monitoring technology is the fungicide, 2-phenylphenol (2-PP).…”

mentioning

confidence: 99%

Engineered Biosensors in an Encapsulated and Deployable System for Environmental Chemical Detection

et al. 2022

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The long-term exposure of low levels of the fungicide, 2-phenylphenol (2-PP), to the environment presents a hazard to human and aquatic health. The cost and difficulty in large-scale production limit the use of existing sensors to detect 2-PP for applications such as personal protection and persistent environmental monitoring of large areas. While advances have been made in using whole cells as biosensors for specific chemical detection, a whole-cell biosensor system with robust biocontainment for field deployment and a strong visual reporter for readouts in the deployed environment has yet to be realized. Here, engineered biosensors in an encapsulated and deployable system (eBEADS) were created to demonstrate a portable, no-power living sensor for detection of 2-PP in the environment. A whole-cell living sensor to detect 2-PP was developed in Escherichia coli by utilizing the 2-PP degradation pathway with an agenetic amplification circuit to produce a visual colorimetric output. To enable field deployment, a physical biocontainment system comprising polyacrylamide alginate beads was designed to encapsulate sensor strains, support long-term viability without supplemental nutrients, and allow permeability of the target analyte. Integration of materials and sensing strains has led to the development of a potential deployable end-to-end living sensor that, with the addition of an amplification circuit, has up to a 66-fold increase in β-galactosidase reporter output over non-amplified strains, responding to as little as 1 μM 2-PP while unencapsulated and 10 μM 2-PP while encapsulated. eBEADS enable sensitive and specific in-field detection of environmental perturbations and chemical threats without electronics.

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The Integration of Whole-Cell Biosensors for the Field-Ready Electrochemical Detection of Arsenic

Cited by 9 publications

References 42 publications

Review of analytical techniques for arsenic detection and determination in drinking water

Review of analytical techniques for arsenic detection and determination in drinking water

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Engineered Biosensors in an Encapsulated and Deployable System for Environmental Chemical Detection

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