The initial and subsequent inflammatory events during calcium oxalate lithiasis

Yuen, Jean Tak Alice Loke; Gohel, Mayur Danny I.; Poon, N.W.; Shum, Dky; Tam, Po‐Chor; Au, Doris W.T.

doi:10.1016/j.cca.2010.03.015

Cited by 27 publications

(31 citation statements)

References 42 publications

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“…The difference between the results of the current study and those of previous studies 19,20 may be also ascribed to other grounds. For example, different cell lines might behave differently when they interact with CaOxa crystals.…”

Section: Morphology Change In Vero Cells Before and After Injurycontrasting

confidence: 99%

“…This phenomenon resulted in unexpected stronger cell viability after adhesion to COD for a set time, leading to the conclusion that "COD microcrystals could promote cell proliferation." 19,20 In fact, studies on COM microcrystals also showed similar results. 19,21 However, in this study, the blank values of cell viability were those after 24 hours of incubation without microcrystal addition.…”

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 57%

“…19,20 In fact, studies on COM microcrystals also showed similar results. 19,21 However, in this study, the blank values of cell viability were those after 24 hours of incubation without microcrystal addition. The experiments for different adhesion times (24,18,12,6, and 2 hours) started at 0, 6, 12, 18, and 22 hours, respectively.…”

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 57%

“…19,20 In these reports, the cell viability at 0 hours was selected as the blank value. Owing to the strong self-repair ability and self-proliferation capability of the cells (including control group and injury group), the cell viability value of selfproliferation was increased with increasing adhesion time.…”

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 99%

“…For example, different cell lines might behave differently when they interact with CaOxa crystals. In previous studies, human kidney-2 cells and Madin-Darby canine kidney cells were used, 19,20 whereas Vero cells were used in the current study. Moreover, different sized CaOxa crystals were used.…”

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 99%

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Interaction between submicron COD crystals and renal epithelial cells

Peng¹,

Ouyang²,

Yao³

et al. 2012

IJN

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Objectives: This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 ± 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation. Methods: Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. Scanning electron microscopy was used to observe Vero-COD adhesion. Inductively coupled plasma emission spectrometry was used to quantitatively measure the amount of adhered COD microcrystals. Nanoparticle size analyzer and laser scanning confocal microscopy were performed to measure the change in the zeta potential on the Vero cell surface and the change in osteopontin expression during the adhesion process, respectively. The level of cell injury was evaluated by measuring the changes in malonaldehyde content, and cell viability during the adhesion process. Results: The adhesion capacity of Vero cells in the injury group to COD microcrystals was obviously stronger than that of Vero cells in the control group. After adhesion to COD, cell viability dropped, both malonaldehyde content and cell surface zeta potential increased, and the fluorescence intensity of osteopontin decreased because the osteopontin molecules were successfully covered by COD. Submicron COD further damaged the cells during the adhesion process, especially for Vero cells in the control group, leading to an elevated amount of attached microcrystals. Conclusion: Submicron COD can further damage injured Vero cells during the adhesion process. The amount of attached microcrystals is proportional to the degree of cell damage. The increased amount of microcrystals that adhered to the injured epithelial cells plays an important role in the formation of early-stage kidney stones.

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Section: Morphology Change In Vero Cells Before and After Injurycontrasting

confidence: 99%

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 57%

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 57%

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 99%

Section: Morphology Change In Vero Cells Before and After Injurymentioning

confidence: 99%

See 3 more Smart Citations

Interaction between submicron COD crystals and renal epithelial cells

Peng¹,

Ouyang²,

Yao³

et al. 2012

IJN

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A hypothesis of calcium stone formation: an interpretation of stone research during the past decades

Tiselius

2011

Urol Res

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An interpretation of previous and recent observation on calcium salt crystallization and calcium stone formation provide the basis for formulation of a hypothetical series of events leading to calcium oxalate (CaOx) stone formation in the urinary tract. The various steps comprise a primary precipitation of calcium phosphate (CaP) at high nephron levels, establishment of large intratubular and/or interstitial (sub-epithelial) aggregates of CaP. These crystal masses subsequently might be dissolved during periods with low urine pH. On the denuded surface of subepithelial or intratubularly trapped CaP, release of calcium ions can result in very high ion-activity products of CaOx, particularly during simultaneous periods with peaks of CaOx supersaturation. Crystals of CaOx may result from nucleation in the macromolecular environment surrounding the apatite crystal phase. In the presence of low pH, low citrate and high ion-strength of urine, formation of large CaOx crystal masses can be accomplished by self-aggregation of Tamm-Horsfall mucoprotein. Following dislodgment of the initially fixed CaOx stone embryo, the further development into to clinically relevant stone is accomplished by CaOx crystal growth and CaOx crystal aggregation of the retained stone material. The latter process is modified by a number of inhibitors and promoters present in urine. The retention of the stone is a consequence of anatomical as well as hydrodynamic factors.

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The impacts of metabolic syndrome on the risk of severe urolithiasis

Xie

Diao

et al. 2022

Urolithiasis

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The initial and subsequent inflammatory events during calcium oxalate lithiasis

Cited by 27 publications

References 42 publications

Interaction between submicron COD crystals and renal epithelial cells

Interaction between submicron COD crystals and renal epithelial cells

A hypothesis of calcium stone formation: an interpretation of stone research during the past decades

The impacts of metabolic syndrome on the risk of severe urolithiasis

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