The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes

Du, Zhenhong; Qiu, Zhilei; Wang, Zhankun; Wang, Xinsheng

doi:10.3892/etm.2017.5359

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Diabetes is a common chronic disease with enhanced glucose levels occurring in the long term, which have an impact on the body and exert a number of negative effects [1]. In diabetes mellitus, glucose lipid metabolism disorders, ischemia hypoxia, inflammatory stimulation, and growth factors all promote angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway

Gao

Qin

Fang

et al. 2019

Journal of Diabetes Research

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Background. Type 2 diabetes mellitus (T2DM) has become a chronic disease, serious harm to human health. Complications of the blood pipe are the main cause of disability and death in diabetic patients, including vascular lesions that directly affects the prognosis of patients with diabetes and survival. This study was to determine the influence of high glucose and related mechanism of vascular lesion of type 2 diabetes mellitus pathogenesis. Methods. In vivo aorta abdominalis of GK rats was observed with blood pressure, heart rate, hematoxylin and eosin (H&E), Masson, and Verhoeff staining. In vitro cells were cultured with 30 mM glucose for 24 h. RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. Immunofluorescence staining was used to detect the expression of PTEN, PI3K, Akt, and VEGF. PI3K and Akt phosphorylation levels were detected by Western blot analysis. Results. Heart rate, systolic blood pressure, diastolic blood pressure, and mean blood pressure in the GK control group were higher compared with the Wistar control group and no difference compared with the GK experimental model group. Fluorescence intensity of VEGF, Akt, and PI3K in the high-sugar stimulus group was stronger than the control group; PTEN in the high-sugar stimulus group was weakening than the control group. VEGF, Akt, and PI3K mRNA in the high-sugar stimulus group were higher than the control group; protein expressions of VEGF, Akt, and PI3K in the high-sugar stimulus group were higher than the control group. PTEN mRNA in the high-sugar stimulus group was lower than the control group. Protein expression of PTEN in the high-sugar stimulus group was lower than the control group. Conclusions. Angiogenesis is an important pathogenesis of T2DM vascular disease, and PTEN plays a negative regulatory role in the development of new blood vessels and can inhibit the PI3K/Akt signaling pathway.

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Section: Introductionmentioning

confidence: 99%

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway

Gao

Qin

Fang

et al. 2019

Journal of Diabetes Research

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The protective effect of pregabalin and xanthenone on testicular ischemia/reperfusion injury in rats

Abdel‐Fattah,

Ahmed,

Saleh

et al. 2024

Fundamemntal Clinical Pharma

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BackgroundTorsion of the spermatic cord is a hazardous and common urologic issue. The current work evaluates the possible protective effect of pregabalin (PGB) and xanthenone (XAN) in testicular ischemia/reperfusion injury induced by testicular torsion/detorsion in rats.Materials and methodsSeven groups of adult male Wistar albino rats were allocated randomly into seven groups, namely, sham control, torsion/detorsion (T/D), PGB 50 mg/kg, PGB 100 mg/kg, XAN 1 mg/kg, XAN 2 mg/kg, and PGB 50 mg/kg plus XAN 1 mg/kg groups. Serum cholesterol and testosterone levels were determined. Also, the levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD), tumor necrosis factor‐α (TNF‐α), nuclear factor kappa B (NF‐қB), angiotensin (Ang) II, Ang‐(1–7), and angiotensin‐converting enzyme2 (ACE2) were assessed in testicular tissue. Immunohistochemical analysis of heme oxygenase‐1 (HO‐1) and caspase‐3 was performed. Finally, the histopathological examination of the testicular tissues was performed.ResultsThe PGB 50 mg/kg, PGB 100 mg/kg, XAN 1 mg/kg, XAN 2 mg/kg, and PGB 50 mg/kg plus XAN 1 mg/kg groups showed a significant decrease in serum cholesterol, MDA, NO, TNF‐α, NF‐қB, and Ang‐II levels coupled with a significant increase in both testosterone and ACE2 expression. Furthermore, all test groups showed a significant improvement in the histopathological picture with a reduction in caspase‐3 and an increase in HO‐1 immunoexpression in testicular tissue.ConclusionPGB and XAN may have promising effects on preventing testicular T/D injury through antioxidant, anti‐inflammatory, and antiapoptotic actions.

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Study the effect of genistein on reproductive functions in male diabetic rats

Amer

Abouda

El-Saka

et al. 2022

Tanta Medical Journal

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Background Diabetes mellitus impairs the reproduction functions through induction of oxidative stress. Genistein is a nonsteroidal estrogenic isoflavone that has been found in plants as soybean. It has controversial effects on male reproductive functions. Aim This study was designed to explore the effect of genistein on reproductive function in male diabetic rats. Materials and methods Fifty adult male Wistar rats were divided into five equal groups: control group, diabetic control group, diabetic treated with genistein 10 mg/kg intraperitoneally (Gen10) group, diabetic treated with genistein 50 mg/kg (Gen50) group, and diabetic treated with genistein 100 mg/kg (Gen100) group. At the end of the experiment, body weight and food intake were determined. The collected blood samples were used for determination of serum glucose, insulin, testosterone, follicle-stimulating hormone, and luteinizing hormone levels. Also, sperm count, viability, motility, and testicular weight were determined. In addition, testicular caspase-3, malondialdehyde, glutathione peroxidase, and catalase were determined. Results In the diabetic group, there was significant deterioration of the studied parameters. All the studied parameters in Gen50 and Gen100 groups were significantly improved as compared with the Gen10 group. Conclusion Genistein ameliorated the reproductive dysfunction in diabetic male rats through improving glycemic state, insulin secretion, in addition to its antioxidant, and anti-apoptotic effect on testicular tissue.

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The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes

Cited by 3 publications

References 21 publications

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway

The protective effect of pregabalin and xanthenone on testicular ischemia/reperfusion injury in rats

Study the effect of genistein on reproductive functions in male diabetic rats

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