The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation

Yano, Akira; Kikuchi, Susumu; Yamashita, Yoshihisa; Sakamoto, Yuichi; Nakagawa, Yuko; Yamaguchi, Yasuo

doi:10.1007/s00253-009-2323-y

Cited by 19 publications

(31 citation statements)

References 27 publications

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“…This value reveals the composition of the glucans, synthesized by the S. mutans. 8) The streptococcal glucan biofilms may be composed of approximately 60-70 mol% α-1,3-glucosidic linkages. 2,8,9) The catalytic preference of our presented enzyme for hydrolysis of biofilms was similar to that of mutanase of Trichoderma harzianum.…”

Section: Mutanase Of P Humicus From Fermented Food Hydrolyzed Insolumentioning

confidence: 99%

Mutanase of Paenibacillus humicus from Fermented Food Has a Potential for Hydrolysis of Biofilms Synthesized by Streptococcus mutans

Tsumori

Shimamura

Sakurai

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Mutanase is an α-1,3-glucanase that cleaves the α-1,3-glucosidic linkages of glucan polysaccharides termed mutan. There has been interest in mutanase as it has the potential to be considered in preventive medicine. Previously, we identified the mutanase of Paenibacillus humicus from fermented soybeans and observed that the enzyme hydrolyzed insoluble glucans and mutan in vitro. Here we focused on the ability of mutanase in preventing dental caries. We proposed that the enzyme participates in the degradation of insoluble glucan biofilms in microplates, which were used as a model of biofilms on tooth surface. In this study, we investigated the potential of mutanase to hydrolyze insoluble glucan biofilms synthesized by a typical cariogenic streptococcus, Streptococcus mutans 10449, in 96-well microplates. The mutanase specifically hydrolyzed insoluble glucan biofilms composed of insoluble glucans synthesized by S. mutans. The mutanase hydrolyzed approximately 60% of S. mutans glucan biofilms in the wells and liberated oligosaccharides from the glucan films. We observed that recombinant mutanase could hydrolyze mutan biofilms, and the enzyme was characterized as an endo-α-1,3-glucanase because its hydrolysis of mutan biofilms, resulted in the predominant liberation of α-1,3-glucan tetrasaccharides. The present study suggests that Paenibacillus mutanase from fermented soybeans has potential application in preventive medicine because of its ability to degrade oral insoluble glucan biofilms.

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Section: Mutanase Of P Humicus From Fermented Food Hydrolyzed Insolumentioning

confidence: 99%

Mutanase of Paenibacillus humicus from Fermented Food Has a Potential for Hydrolysis of Biofilms Synthesized by Streptococcus mutans

Tsumori

Shimamura

Sakurai

et al. 2011

JOURNAL OF HEALTH SCIENCE

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“…However, without targeting and reducing dental plaque, fluoride products are unable to completely inhibit dental caries (Fejerskov 2004). Natural products and derivatives, such as green tea catechins (Hirasawa et al 2006), cranberry constituents , Galla chinensis (Xie et al 2008), citrus lemon oil (Liu et al 2013), mushroom extracts (Yano et al 2010), and several bioactive macromolecules (Shang et al 2014;Lee and Park 2015), have been reported to be effective against the growth and adherence of S. mutans. In addition, small molecules, such as tt-farnesol (Koo et al 2003), walkmycin C (Eguchi et al 2011), and anthraquinones (Coenye et al 2007), have been shown to inhibit the growth of cariogenic bacteria, and even the development of caries, but few of these compounds target S. mutans biofilms.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

Chen

Ren

Zhou

et al. 2015

Appl Microbiol Biotechnol

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Dental caries, a biofilm-related oral disease, is a result of disruption of the microbial ecological balance in the oral environment. Streptococcus mutans, which is one of the primary cariogenic bacteria, produces glucosyltransferases (Gtfs) that synthesize extracellular polysaccharides (EPSs). The EPSs, especially water-insoluble glucans, contribute to the formation of dental plaque, biofilm stability, and structural integrity, by allowing bacteria to adhere to tooth surfaces and supplying the bacteria with protection against noxious stimuli and other environmental attacks. The identification of novel alternatives that selectively inhibit cariogenic organisms without suppressing oral microbial residents is required. The goal of the current study is to investigate the influence of an oxazole derivative on S. mutans biofilm formation and the development of dental caries in rats, given that oxazole and its derivatives often exhibit extensive and pharmacologically important biological activities. Our data shows that one particular oxazole derivative, named 5H6, inhibited the formation of S. mutans biofilms and prevented synthesis of extracellular polysaccharides by antagonizing Gtfs in vitro, without affecting the growth of the bacteria. In addition, topical applications with the inhibitor resulted in diminished incidence and severity of both smooth and sulcal surface caries in vivo with a lower percentage of S. mutans in the animals' dental plaque compared to the control group (P<0.05). Our results showed that this oxazole derivative has the capacity to inhibit biofilm formation and cariogenicity of S. mutans.

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“…These microorganisms form biofilms on the tooth surface by attachment to the acquired pellicle on the enamel and adhesion to the tooth by cell to cell interactions. [20] The shiitake extracts could reduce preformed biofilms in the presence of dextranase which is a water-soluble α-(1,6)-linked glucan. This dextran degrading enzyme, dextranase, has been used in the treatment to reduce the formation of dental plaque.…”

Section: Introductionmentioning

confidence: 99%

“…This dextran degrading enzyme, dextranase, has been used in the treatment to reduce the formation of dental plaque. [20] An in vitro dental caries biofilm model was developed in combination with cariogenic microorganisms with dental hard tissue substratum (dentin or enamel) allowing modeling of frequent acid challenges by sucrose pulsing with a constant depth film fermentor. The amount of mineral loss quantifies anticariogenicity.…”

Section: Introductionmentioning

confidence: 99%

The unexplored anticaries potential of shiitake mushroom

J¹,

Vinay²,

Jha³

et al. 2016

Phcog Rev

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Keeping an eye the escalating costs of dental services, the treatment cost of the consequences of dental caries can be reduced to manageable proportions by preventive measures aimed at decreasing the prevalence. One such measure is by increasing the consumption of caries preventive foods. Recently, there has been an upsurge of interest in mushrooms not only as a healthy food but also as a caries preventive food. The most common type of mushroom, Lentinula edodes also called as shiitake, is studied in-depth for its oral health benefits. The cultivation of shiitake dates way back to 1100 A.D. during the rule of Sung dynasty which is replaced by more modern and efficient sawdust substrate log cultures lately. Shiitake mushroom extract can be isolated in various forms such as freeze dried, oil, and ethyl acetate extracts. Various biologically active compounds such as erythritol, copalic acid, adenosine, carvacrol, and many more are responsible for this mushroom's antimicrobial activity. Anticariogenicity can be attributed to the induction of the detachment of cariogenic microorganisms from hydroxyapatite, changes in cell surface hydrophobicity, bactericidal activity, and disruption of signal transduction in Streptococcus mutans as proved through various in vivo and in vitro studies. Apart from these benefits, it has tremendous potential to be used as an antioxidant, anticancer, antigingivitis, antifungal, and antiviral agent. The one and only known adverse reaction due to shiitake mushroom consumption is the eruption of pruritic erythematous papules termed as shiitake dermatitis. This review highlights the unexplored anticaries potential of one such useful bioactive metabolite-shiitake mushroom.

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The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation

Cited by 19 publications

References 27 publications

Mutanase of Paenibacillus humicus from Fermented Food Has a Potential for Hydrolysis of Biofilms Synthesized by Streptococcus mutans

Mutanase of Paenibacillus humicus from Fermented Food Has a Potential for Hydrolysis of Biofilms Synthesized by Streptococcus mutans

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

The unexplored anticaries potential of shiitake mushroom

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