The inhibitory effect of reserpine on the active sodium transport across the frog bladder

Marumo, Fumiaki; Mishina, Takao; Asano, Yoshihide; Tashima, Yohtalou

doi:10.1007/bf00583623

Cited by 20 publications

(2 citation statements)

References 10 publications

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“…More recent work has challenged this interpretation [4,21,24,26] and showed in addition an effect of harmaline on other ATPases [26]. Our results are compatible with the alleged inhibition of the (Na+ K)-ATPase from the cytoplasmic side of the plasma membrane, but do not provide a clue for a specific interaction between harmaline and Na.…”

Section: Discussionsupporting

confidence: 71%

Vasopressin-like effects of a hallucinogenic drug-harmaline-on sodium and water transport

Sousa

Grosso

1978

J. Membrain Biol.

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To determine if harmala alkaloids affect transport systems other than (Na +K)-ATPase, effects of harmaline on Na and water fluxes were studied in amphibian skins. Net Na flux was evaluated from short-circuit current, and water flux monitored with automatic, volumetric methods. At 2 to 5 mM, harmaline consistently inhibited SCC and prevented the natriferic effects of oxytocin and norepinephrine. However, at 0.1 to 0.5 mM, harmaline produced an increase in SCC inhibitable with amiloride. The stimulatory effects of harmaline and oxytocin were either nonadditive or additive depending on whether the hallucinogen was present in the inner solution or in the outer solution bathing the skin, respectively. Water flow was not modified by harmaline on the outer medium. In contrast, addition of the drug to the inner medium elicited a conspicuous, sustained, vasopressin-like, hydrosmotic effect, comparable to and competive with those of vasopressin and norepinephrine. The ensemble of these results suggests that harmaline may affect three distinct transport systems: (i) the Na pump; (ii) the cyclic nucleotide system; (iii) the Na entry pathway at the outer membrane of the skin that is also activated by agents such as diphenylhydantoin, lanthanides and propranolol.

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Section: Discussionsupporting

confidence: 71%

Vasopressin-like effects of a hallucinogenic drug-harmaline-on sodium and water transport

Sousa

Grosso

1978

J. Membrain Biol.

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“…Short-circuit current (SCC) was measured by the method of Ussing and Zerahn [7], The area of the chamber orifice for measurement of the osmotic water flow was 2.66 cm2, and for the SCC it was 3.16 cm2. To prevent mechanical distortion of the bladder membrane, both orifices of the chamber were covered with nylon mesh, so that the bladder was sandwiched between two mesh discs as described pre viously [3],…”

Section: Water Permeability and Sodium Transport In The Frog Bladdermentioning

confidence: 99%

Effects of Diazoxide and Hydrochlorothiazide on Water Permeability and Sodium Transport in the Frog Bladder

Marumo¹,

Mishina²,

Shimada³

1982

Pharmacology

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The effects of diazoxide and hydrochlorothiazide on vasopressin-induced increments in osmotic water flow and sodium transport across the frog bladder were studied. Diazoxide enhanced the vasopressin-induced osmotic water flow of the bladder, but did not affect the cyclic AMP- or theophylline-induced water flow. Hydrochlorothiazide did not affect the vasopressin-induced water flow. Our results suggest that diazoxide increased the water flow by inhibiting the activity of phosphodiesterase in bladder epithelial cells, whereas hydrochlorothiazide did not. On the other hand, both drugs suppressed the short-circuit current of the bladder membrane and inhibited the NaK-dependent ATPase activity of the kidney cells. These results suggest that both drugs decreased sodium transport in the bladder by inhibiting NaK-dependent ATPase activity.

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The Renal Proximal Tubule

Silverman¹,

Turner²

1979

Biomembranes

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The inhibitory effect of reserpine on the active sodium transport across the frog bladder

Cited by 20 publications

References 10 publications

Vasopressin-like effects of a hallucinogenic drug-harmaline-on sodium and water transport

Vasopressin-like effects of a hallucinogenic drug-harmaline-on sodium and water transport

Effects of Diazoxide and Hydrochlorothiazide on Water Permeability and Sodium Transport in the Frog Bladder

The Renal Proximal Tubule

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