The Inhibitory Effect of Propofol on Bovine Lactoperoxidase

Abstract

Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro effect of propofol on lactoperoxidase (LPO; E.C. 1.11.1.7) obtained from bovine milk. Lactoperoxidase was purified with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Lactoperoxidase was purified with a yield of 21.6%, a specific activity of 34 EU/mg proteins and 14.7-fold purification. One enzyme unit is defined as the oxidation of 1 micromol ABTS per min under the assay condition (25( degrees )C, pH: 6.0). To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The effect of propofol on lactoperoxidase were determined using 2,2'-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate. The IC(50) value of propofol was found as 15.97 microM. Also, K(i) constant for propofol was 3.72 microM and propofol was found as competitive inhibitor.

The effects of some bromophenols on human carbonic anhydrase isoenzymes

“…The bromophenol 11, which had two methoxy (-OH) and a bromine (-Br) groups, was the best hCA II inhibitor (K i : 2.01 ± 0.52 nM). Phenolic compounds have a lot of nutritional and pharmacological properties including anti-oxidant properties [62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80][81] and enzymes inhibition [82][83][84][85][86] . However, all bromophenol derivatives (1-12) have shown similar hCA II inhibition properties.…”

Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase

“…For each tetrahydropyrimidine-5-carboxylates (1-3), an activity (%) -[Tetrahydropyrimidine-5-carboxylates] was drawn using conventional polynominal regression software. The half maximal inhibitory concentration (IC 50 ) of all tetrahydropyrimidine-5-carboxylates (1-3) was calculated from graphs 88 . IC 50 values are measures of the effectiveness of novel tetrahydropyrimidine-5-carboxylates (1-3) in inhibiting both CA isoenzymes.…”

Synthesis and inhibitory properties of some carbamates on carbonic anhydrase and acetylcholine esterase

“…Subsequently, the proteins from the column were spectrophotometrically determined at 280 nm [79][80][81][82][83][84][85] . For determination of the purity of the hCA isoenzymes, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), having 10 and 3% acrylamide as an eluent and packing gel, respectively, with 0.1% SDS [86][87][88][89][90] , was performed, through which a single band was observed for each isoenzyme.…”