The inhibitory effect of NUCB2/nesfatin-1 on appetite regulation of Siberian sturgeon (Acipenser baerii Brandt)

Zhang, Xin; Wang, Shuyao; Chen, Hu; Tang, Ni; Qi, Jinwen; Wu, Yingliang; Hao, Jin; Tian, Zhengzhi; Wang, Bin; Chen, Defang; Li, Zhiqiong

doi:10.1016/j.yhbeh.2018.06.008

Cited by 17 publications

(6 citation statements)

References 45 publications

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“…Feeding C. nasus had a significant up‐regulation of NUCB2 mRNA in the stomach compared to non‐feeders, indicating a fast response to nutrient availability. As reported in the Siberian sturgeon ( Acipenser baerii Brandt), NUCB2 acts as a satiety factor to inhibit food intake (Zhang et al, 2018). Goldfish and zebrafish also exhibited a significant increase in NUCB2 mRNA levels in the brain after feeding (Gonzalez, Kerbel, Chun, & Unniappan, 2010; Hatef, Shajan, & Unniappan, 2015).…”

Section: Discussionmentioning

confidence: 90%

Insights into response to food intake in anadromous Coilia nasus through stomach transcriptome analysis

Yin

Fang

et al. 2020

Aquac Res

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Coilia nasus is a valuable commercial migratory fish species with ecological and economic importance in China. To explore the molecular mechanisms underlying the response to food intake, the stomach transcriptomes of feeding and non‐feeding C. nasus were analysed via RNA‐seq. A total of 610,640,864 clean reads were obtained, and annotation to six databases identified 63,567 unigenes. Comparative analysis of the gene expression in feeding and non‐feeding C. nasus identified 1,968 differentially expressed genes (p < .05), including 1,180 up‐regulated and 788 down‐regulated genes. Our results showed that stomach distention activated the vagal afferent neurons, resulting in satiation. Several of the most relevant appetite genes (e.g. leptin receptor [LepR], somatostatin [SS], nucleobindin‐2 [NUCB2], 5‐hydroxytryptamine receptor [5‐HTR], growth hormone receptor [GHR]) were also involved in the regulation of food intake and led to the termination of feeding. Furthermore, significant differences were observed in the genes encoding key enzymes involved in metabolism such as the tricarboxylic acid cycle (TCA) pathways, fatty acid synthesis and glycolysis, indicating that C. nasus utilizes food for its energy and nutrient contents to support life and gonadal development. These findings provide valuable insights into the mechanisms underlying appetite and metabolic regulation in anadromous fish and create a foundation for further research on C. nasus artificial breeding and migration energetics.

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Section: Discussionmentioning

confidence: 90%

Insights into response to food intake in anadromous Coilia nasus through stomach transcriptome analysis

Yin

Fang

et al. 2020

Aquac Res

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“…Nucleobindin‐2, also called nesfatin‐1 (NUCB2), is a calcium and DNA binding peptide. NUCB2, the levels of which increase after food intake and decrease during fasting, has food intake suppression effects by direct interaction with leptin, which in turn reduces bodyweight gain [43, 44].…”

Section: Resultsmentioning

confidence: 99%

Proteomic profiling of metformin effects in 3T3‐L1 adipocytes by SILAC‐based quantification

et al. 2022

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Metformin is a common and generally the first medication prescribed for treatment of type 2 diabetes. Its mechanism involves affecting pathways that regulate glucose and lipid metabolism in metabolic cells such as that of muscle and liver cells. In spite of various studies exploring its effects, the proteome changes in adipocytes in response to metformin remains poorly understood. In this study, we performed stable isotope labeling by amino acids in cell culture (SILAC)‐based quantitative proteomic profiling to study the effects of metformin specifically on 3T3‐L1 adipocytes. We define proteins that exhibited altered levels with metformin treatment, 400 of them showing statistically significant changes in our study. Our results suggest that metformin affects not only the PPAR signaling pathway, as well as glucose and lipid metabolism, but also protein folding, endoplasmic reticulum stress, negative regulation of appetite, and one‐carbon folate metabolism in adipocytes. This proteomic investigation provides important insight into effects of metformin in adipocytes.

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“…They are always in a dynamic balance through the increase and decrease of expression to complete the feeding regulation of animal. Recently, several genes involved in promoting (npy (64) ) or inhibiting feeding (cart (38) , nucb2 (39) , ucn3 (35) , cck (37) and pyy (34) ) have been identified in Siberian sturgeon by our group. These neuropeptides and peptides secreted from other tissues play important role in regulating appetite.…”

Section: Effects Of Neuromedin U On Appetite Factors Expressionsmentioning

confidence: 98%

“…Several appetite factors have been identified in Siberian sturgeon by our group, such as PYY (34) , UCN3 (35) , Apelin (36) , CCK (37) , CART (38) and NUCB2/Nesfatin-1 (39) . However, no available data about nmu in Siberian sturgeon have been found.…”

mentioning

confidence: 91%

Neuromedin U regulates food intake of Siberian sturgeon through the modulation of central and peripheral appetite factors

et al. 2022

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Neuromedin U (NMU) has a critical function on the regulation of food intake in mammals, while the information is little in teleost. To investigate the function of NMU on appetite regulation of Siberian sturgeon (Acipenser baerii), this study firstly cloned nmu cDNA sequence that encoded 154 amino acids including NMU-25 peptide. Besides, the results showed that nmu mRNA was widely distributed in various tissues especially in the hypothalamus and telencephalon. The results of nutritional status (pre-feeding and post-feeding, fasting and re-feeding) experiments showed that nmu mRNA expression was significantly decreased at 1 and 3 h after feeding in different brain regions. Similarly, after feeding, the expression of nmu significantly decreased in peripheral tissues. Moreover, nmu expression in the hypothalamus was significantly increased after fasting 1 day, but decreased after fasting 17 days, which was significantly reversed after re-feeding. However, other brain regions like telencephalon and peripheral tissues like esophagus, intestinum valvula and liver have different change patterns. Further study showed that acute i.c.v. and i.p. injection of NMU and chronic i.p. injection of NMU significantly reduced the food intake in a dose dependent mode. In addition, the expressions of several critical appetite factors (nmu, aplein, cart, cck, ghrelin, npy, nucb2, pyy and ucn3) were significantly affected by acute NMU-25 administration in the hypothalamus, intestinum valvula and liver. These results indicate that NMU-25 has the anorexigenic function on food intake by affecting different appetite factors in Siberian sturgeon, which provides a foundation for further exploring the appetite regulation networks in fish.

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The inhibitory effect of NUCB2/nesfatin-1 on appetite regulation of Siberian sturgeon (Acipenser baerii Brandt)

Cited by 17 publications

References 45 publications

Insights into response to food intake in anadromous Coilia nasus through stomach transcriptome analysis

Insights into response to food intake in anadromous Coilia nasus through stomach transcriptome analysis

Proteomic profiling of metformin effects in 3T3‐L1 adipocytes by SILAC‐based quantification

Neuromedin U regulates food intake of Siberian sturgeon through the modulation of central and peripheral appetite factors

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