The inhibitory activity of Lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin

Jara, Solange; Sánchez, M.; Vera, Rodrigo; Cofre, Jaime; Castro, Erica

doi:10.1016/j.anaerobe.2011.07.008

Cited by 47 publications

(44 citation statements)

References 20 publications

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“…While there is limited information on mechanisms for initial establishment of lactobacilli in the mouth of infants and the role of breast-milk as a source of oral bacteria (21, 22), culturing lactobacilli only from breastfed infants, and q-PCR detection of L. gasseri in breastfed infants, in the current study is consistent with detection of lactobacilli in breast milk (43) and their transmission from the nipple and from milk (21, 22, 44). The finding that only a fraction of breastfed infants have viable lactobacilli in saliva may reflect decreasing content of lactobacilli in milk by lactation age.…”

Section: Discussionsupporting

confidence: 80%

Oral Microbial Profile Discriminates Breast‐fed From Formula‐fed Infants

Holgerson

Vestman²,

Claesson³

et al. 2013

J. pediatr. gastroenterol. nutr.

143

128

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Objectives Little is known about the impact of diet on the oral microbiota of infants although diet is known to affect the gut microbiota. The aims of the present study were to compare the oral microbiota in breastfed and formula-fed infants, and investigate growth inhibition of streptococci by infant-isolated lactobacilli. Subjects and Methods 207 mothers consented to participation of their three-month old infants. 146 (70.5%) infants were exclusively and 38 (18.4%) partially breastfed, and 23 (11.1%) were exclusively formula-fed. Saliva from all infants was cultured for Lactobacillus species, with isolate identifications from 21 infants. Lactobacillus isolates were tested for their ability to supress Streptococcus mutans and Streptococcus sanguinis. Oral swabs from 73 infants were analysed by the Human Oral Microbe Identification Microarray (HOMIM) and by q-PCR for Lactobacilius gasseri. Results Lactobacilli were cultured from 27.8% of exclusively and partially breastfed infants, but not from formula-fed infants. The prevalence of 14 HOMIM detected taxa, and total salivary lactobacilli counts differed by feeding method. Multivariate modelling of HOMIM detected bacteria and possible confounders clustered samples from breastfed infants separately from formula-fed infants. The microbiota of breastfed infants differed based on vaginal or C-section delivery. Isolates of Lactobacillus plantarum, L. gasseri and Lactobacillus vaginalis inhibited growth of the cariogenic S. mutans and the commensal S. sanguinis: L. plantarum > L. gasseri > L. vaginalis. Conclusion The microbiota of the mouth differs between breastfed and formula-fed three-month-old infants. Possible mechanisms for microbial differences observed include species suppression by lactobacilli indigenous to breast milk.

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Section: Discussionsupporting

confidence: 80%

Oral Microbial Profile Discriminates Breast‐fed From Formula‐fed Infants

Holgerson

Vestman²,

Claesson³

et al. 2013

J. pediatr. gastroenterol. nutr.

143

128

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“…and Serratia spp. [32]. Discriminant analysis could also clearly discriminate bacterial communities from samples obtained from different farms (Figure 2IV).…”

Section: Discussionmentioning

confidence: 92%

Microbiota of Cow’s Milk; Distinguishing Healthy, Sub-Clinically and Clinically Diseased Quarters

et al. 2014

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The objective of this study was to use pyrosequencing of the 16S rRNA genes to describe the microbial diversity of bovine milk samples derived from clinically unaffected quarters across a range of somatic cell counts (SCC) values or from clinical mastitis, culture negative quarters. The obtained microbiota profiles were used to distinguish healthy, subclinically and clinically affected quarters. Two dairy farms were used for the collection of milk samples. A total of 177 samples were used. Fifty samples derived from healthy, culture negative quarters with a SCC of less than 20,000 cells/ml (group 1); 34 samples derived from healthy, culture negative quarters, with a SCC ranging from 21,000 to 50,000 cells/ml (group 2); 26 samples derived from healthy, culture negative quarters with a SCC greater than 50,000 cells/ml (group 3); 34 samples derived from healthy, culture positive quarters, with a SCC greater than 400,000 (group 4, subclinical); and 33 samples derived from clinical mastitis, culture negative quarters (group 5, clinical). Bacterial DNA was isolated from these samples and the 16S rRNA genes were individually amplified and pyrosequenced. All samples analyzed revealed great microbial diversity. Four bacterial genera were present in every sample obtained from healthy quarters (Faecalibacterium spp., unclassified Lachnospiraceae, Propionibacterium spp. and Aeribacillus spp.). Discriminant analysis models showed that samples derived from healthy quarters were easily discriminated based on their microbiota profiles from samples derived from clinical mastitis, culture negative quarters; that was also the case for samples obtained from different farms. Staphylococcus spp. and Streptococcus spp. were among the most prevalent genera in all groups while a general multivariable linear model revealed that Sphingobacterium and Streptococcus prevalences were associated with increased 10 log SCC. Conversely, Nocardiodes and Paenibacillus were negatively correlated, and a higher percentage of the genera was associated with a lower 10 log SCC.

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“…Antagonistic properties of probiotic organisms have been demonstrated in vitro and in vivo in bifidobacteria, lactobacilli and combinations of probiotics (Neville and O'Toole ; Jara et al . ; Langa et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…; Jara et al . ), and from food and environmental sources (Neville and O'Toole ). Lactobacillus salivarius displays a high level of genomic diversity (Raftis et al .…”

Section: Discussionmentioning

confidence: 99%

Identification of L actobacillus strains with probiotic features from the bottlenose dolphin ( T ursiops truncatus )

et al. 2013

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AimsIn order to develop complementary health management strategies for marine mammals, we used culture-based and culture-independent approaches to identify gastrointestinal lactobacilli of the common bottlenose dolphin, Tursiops truncatus.Methods and ResultsWe screened 307 bacterial isolates from oral and rectal swabs, milk and gastric fluid, collected from 38 dolphins in the U.S. Navy Marine Mammal Program, for potentially beneficial features. We focused our search on lactobacilli and evaluated their ability to modulate TNF secretion by host cells and inhibit growth of pathogens. We recovered Lactobacillus salivarius strains which secreted factors that stimulated TNF production by human monocytoid cells. These Lact. salivarius isolates inhibited growth of selected marine mammal and human bacterial pathogens. In addition, we identified a novel Lactobacillus species by culture and direct sequencing with 96·3% 16S rDNA sequence similarity to Lactobacillus ceti.ConclusionsDolphin-derived Lact. salivarius isolates possess features making them candidate probiotics for clinical studies in marine mammals.Significance and Impact of the StudyThis is the first study to isolate lactobacilli from dolphins, including a novel Lactobacillus species and a new strain of Lact. salivarius, with potential for veterinary probiotic applications. The isolation and identification of novel Lactobacillus spp. and other indigenous microbes from bottlenose dolphins will enable the study of the biology of symbiotic members of the dolphin microbiota and facilitate the understanding of the microbiomes of these unique animals.

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The inhibitory activity of Lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin

Cited by 47 publications

References 20 publications

Oral Microbial Profile Discriminates Breast‐fed From Formula‐fed Infants

Oral Microbial Profile Discriminates Breast‐fed From Formula‐fed Infants

Microbiota of Cow’s Milk; Distinguishing Healthy, Sub-Clinically and Clinically Diseased Quarters

Identification of L actobacillus strains with probiotic features from the bottlenose dolphin ( T ursiops truncatus )

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