The inhibition of lamellar hydroxyapatite and lamellar magnetic hydroxyapatite on the migration and adhesion of breast cancer cells

Abstract

Hydroxyapatite nanoparticles have been reported to exhibit potent anti-tumor effects in some cancer cells. In our previous study, we have successfully synthesized two types of hydroxyapatite nanoparticles, laminated hydroxyapatite (L-HAp) and laminated magnetic hydroxyapatite (LM-HAp). In this study, we wanted to investigate the effects of L-HAp and LM-HAp with various concentrations on human breast cancer MDA-MB-231 cells. Cell proliferation was assessed with a MTT colorimetric assay. Scratch and adhesion assays were used to detect the effects of these two materials on migration and adhesion. The expressions of integrin β1 and Akt were measured by Western blotting. Our results showed that L-HAp and LM-HAp had little cell cytotoxicity and significantly reduced cell mobility and adhesion. LM-HAp showed greater inhibitor ability on migration and adhesion of MDA-MB-231 cells. Moreover, results from western blotting showed that L-HAp and LM-HAp impacted the phosphorylation of integrin β1, but showed no regular impact on Akt. This study suggests that L-HAp and LM-HAp may be potential anti-tumor and delivery system for breast cancer therapy.

Novel porous graphene oxide and hydroxyapatite nanosheets-reinforced sodium alginate hybrid nanocomposites for medical applications

“…In a typical process, 0.5 g of sodium dodecyl sulphonate (SDS, C 12 days at room temperature. The resultant precipitate was centrifuged, immersed in deionized water for 3 days, centrifuged again, and finally dried at 60 °C and the lamellar HAp with highly ordered laminated structure was obtained, as reported in our previous work [26,27].…”

“…It should be mentioned that the porosity of all nanocomposites is larger than 85% which is sufficient for bone tissue engineering [35]. HAp [26]. It should be noted that the peak of GO is not observed in the nanocomposites, likely because the amount of GO is too low to be measured.…”

“…The template-assisted synthesis was used to prepare the lamellar HAp as described in our previous work [26]. In a typical process, 0.5 g of sodium dodecyl sulphonate (SDS, C 12 days at room temperature.…”

Polyethylenimine-coated SPION exhibits potential intrinsic anti-metastatic properties inhibiting migration and invasion of pancreatic tumor cells

“…Inorganic nanoparticles, including SPIONs, can impair cell motility in a dose-dependent manner [19][20][21]. The underlying molecular mechanisms that lead to these effects, which might depend on cell type and nanoparticle surface charge and shape [22], are nonetheless poorly understood.…”

“…PEI stand out among polycations for use in gene delivery [11,15,16] due to the 'sponge effect', which leads to early endosome rupture and DNA/RNA release into the cytoplasm. Due to their unique properties [17,18], the PEI/SPION combination has been explored for cancer gene therapy, but the effect of 'naked' PEI-coated SPIONs on cells, in particular on tumor cells, has not been studied thoroughly.Inorganic nanoparticles, including SPIONs, can impair cell motility in a dose-dependent manner [19][20][21]. The underlying molecular mechanisms that lead to these effects, which might depend on cell type and nanoparticle surface charge and shape [22], are nonetheless poorly understood.…”