The inhibition of Fusarium wilt in Chili by Endophytic Fungi isolated from Green Betel (Piper betle L.) Leaf

Abstract

Green Betel (Piper betle L.) leaves are often used as medicine by local people because they contain antibacterial and antifungal substances. These substances can be produced by plant metabolites and are also the results of metabolites produced by endophytic fungi. Endophytic fungus is an organism that is associated with healthy host tissues without causing disease symptoms. Endophytic fungi from betel leaf are used as biocontrol agents against Fusarium oxysporum causes wilt disease that attacks Chili (Capsicum annuum L.), which is one of the main agricultural commodities in Indonesia. Fusarium wilt disease is a disease that significantly reduces crop yields based on crop yields. This research was conducted in a chili farm in Cangkringan, Sleman, Yogyakarta. This study aimed to test the antagonism of endophytic fungi with the dual culture technique. Three types of green betel leaf endophytic fungi were used for dual culture test against Fusarium oxysporum. The observation result showed the highest inhibition ability of 73.37% dual culture method. The inhibitory mechanisms of green betel leaf endophytic fungal isolates include parasitism, antibiosis, and competition. Endophytic fungi of green betel leaf are effective as biocontrol agents of wilt Fusarium chili disease. ©2022 JNSMR UIN Walisongo. All rights reserved.

Antagonistic Activity of Endophytic Fungi Isolated From Aloe Vera Leaves Against Some Plant Pathogenic Fungi

“…The inoculated plates were incubated at a temperature of 27 ° C for 7 days. The diameter of the pathogenic fungus colony was calculated and the inhibition percentage was extracted according to the following equation: Percentage of inhibition % = (control colony diameter -treatment colony diameter) / (control colony diameter) x 100 (49). Detection of the organic compounds present in the extract of endophytic fungi filtrates using the GC-MS device 1/ Preparation of the extract of endophytic fungi filtrates.…”

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

“…Pada penelitian Fusarium graminearum yang diisolasi dari wilayah kaya akan gandum dapat menghasilkan aktivitas selulolitik hingga 320-330 mU/mL pada hari ketujuh hingga kesembilan (Kikot et al, 2010). Isolat ES9 digunakan juga pada penelitian Syaifudin & Kasiamdari (2022) yang menunjukkan Fusarium graminearum mampu menghambat patogen penyebab layu Fusarium pada cabai dengan mekanisme kompetisi sebesar 65,1% pada metode dual culture secara in vitro. Colletotrichum coccodes memiliki aktivitas tertinggi pada hari keempat sebesar 0,029 U/ml.…”