The inhibition of DNA‐dependent RNA synthesis in yoshida ascites cells by bovine follicular fluid

Abstract

Follicular fluid aspirated from large cow follicles inhibits endogenous, DNA-dependent RNA polymerase activity in Yoshida ascites cells. The inhibitory component of follicular fluid is probably a protein and appears to affect specifically the activity of the nucleoplasmic polymerase II.

Protein composition in the fluid of individual bovine follicles

“…It has been suggested that this accessibility might increase with the development of the follicle (Zachariae, 1958 ; Glass, 1961 ; Anderson, 1972), but there is disagreement on this point (Lipner & Smith, 1971 ; Payer, 1975). Peters, Byskov & Faber (1973) have suggested that follicular fluid might inhibit the start of follicular growth in the immature mouse ovary, and it has been shown that bovine follicular fluid contains a component which inhibits RNA synthesis in Yoshida ascites cells in vitro (Moore et al, 1975). Since the component responsible might be a protein, we have studied the concentrations of individual proteins in follicular fluid for comparison with those of serum.…”

Inhibitory effect of follicular fluid on RNA synthesis of rat granulosa cells in vitro

“…luteinization is controlled by an inhibitory factor in the fluid (El-Fouly, Cook, Nekola & Nalbandov, 1970;Channing, 1973) and Moore et al (1975) found an inhibition of the activity of RNA-polymerase of rat Yoshida ascites cells by bovine follicular fluid. Bernard (1975) showed that pig follicular fluid prevents morphological differentiation of rat granulosa cells in vitro, but does not alter their progesterone secretion.…”