The Influence of Follicular Fluid on Progesterone Secretion by Porcine Granulosa Cells in Vitro

Ledwitz-Rigby, Florence; Rigby, B W

doi:10.1007/978-1-4684-3474-3_40

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…Consequently, it is impossible to separate clearly this inhibitor from other substances (see below) which may also inhibit cAMP accumulation under certain circumstances. Preliminary molecular size estimates based on ultrafiltration studies indicated a molecular weight greater than 100000 (Ledwitz- Rigby and Rigby 1979a). If confirmed with more formal estimates, a molecular size in this range would allow clear separation from other inhibitors and provide support for the existence of luteinization inhibitor as a discrete physical entity.…”

Section: Luteinization Inhibitormentioning

confidence: 94%

Peptide Regulators in the Ovarian Follicle

Hammond¹

1981

Aust. Jnl. Of Bio. Sci.

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Data generated within the last several years have shown that follicular fluid contains substances, presumably peptide in nature, which exert potentially important effects on granulosa cells and the oocyte. This review briefly summarizes the current evidence concerning the nature and importance of these putative regulators including the luteinization inhibitor, oocyte maturation inhibitor, inhibitors of the binding of luteinizing hormone and follicle stimulating hormone, stimulators of ornithine decarboxylase and intrafollicular peptides with gonadotrophin-releasing activity. Although the existence of such activities has been clearly demonstrated, the evidence for a regulatory role of these agents in the control of ovarian physiology is not compelling. However, their occurrence must now be taken into account in our attempts to understand the mechanism of follicular growth, differentiation and atresia.

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Section: Luteinization Inhibitormentioning

confidence: 94%

Peptide Regulators in the Ovarian Follicle

Hammond¹

1981

Aust. Jnl. Of Bio. Sci.

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Presence of a low molecular weight inhibitor of succinate-supported cholesterol side chain cleavage in rat ovaries

Toaff

Schleyer

Strauss

1980

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Inhibition by human embryos of mouse granulosa cell progesterone production: development of a sensitive bioassay

Dhawan

Léveillé²,

Vanderhyden³

2000

Human Reproduction

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Reproduction technologies could be improved by the development of methods to evaluate oocyte or embryo quality in a non-invasive, quantitative manner. Since human embryos secrete a factor that inhibits granulosa cell progesterone production, an interspecies bioassay was established to investigate whether the presence of this progesterone-inhibitory factor (PIF) in human embryo-conditioned (HEC) media is related to the health and developmental capacity of the embryos. Oocytes were microsurgically removed from oocyte-cumulus complexes isolated from superovulated mouse ovaries, and the oocytectomized complexes were cultured in HEC media in the presence of follicle stimulating hormone and testosterone. Progesterone accumulation in the media was determined by radioimmunoassay. Despite the potential limitations of very small volumes of HEC media to evaluate, and the need to freeze these media at the source, the bioassay was able to detect PIF activity in HEC media. Most embryos produced PIF activity, but the degree of inhibition was not correlated with the ability of oocytes to be fertilized, nor with embryo morphology or ability to cleave and develop after transfer. These results demonstrate that secretion of PIF by human embryos can be measured by this bioassay and that human PIF can inhibit murine granulosa cell steroidogenesis; however, PIF activity is not correlated with human embryo quality or developmental competence.

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The Influence of Follicular Fluid on Progesterone Secretion by Porcine Granulosa Cells in Vitro

Cited by 6 publications

References 20 publications

Peptide Regulators in the Ovarian Follicle

Peptide Regulators in the Ovarian Follicle

Presence of a low molecular weight inhibitor of succinate-supported cholesterol side chain cleavage in rat ovaries

Inhibition by human embryos of mouse granulosa cell progesterone production: development of a sensitive bioassay

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