The inhibition of 2,3-dichloro-1-propanol on T cell in vitro and in vivo

Lü, Jing; Huang, G.; Zhang, Shuang; Song, Bocui; Wang, Zhenning; Xu, Lan; Zhang, Shuonan; Guan, Shuang

doi:10.1016/j.intimp.2013.06.026

Cited by 6 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR analysis of additional genes, such as HERPUD1 and SQLE, supported these findings. A previous study indicated that 2,3-DCP inhibits T cell activation and downregulates activation of transduction pathways in vitro (mouse T cells purified from splenocytes) and mice in vivo (Lu et al, 2013). However, 2,3-DCP did not significantly affect expression of the T cell activation marker CD69 in the present study.…”

Section: Discussioncontrasting

confidence: 79%

“…A previous study indicated that 2,3-DCP inhibits T cell activation and downregulates activation of transduction pathways in vitro (mouse T cells purified from splenocytes) and mice in vivo (Lu et al, 2013). PCR analysis of additional genes, such as HERPUD1 and SQLE, supported these findings.…”

Section: Discussionmentioning

confidence: 72%

“…In total we selected nine known immunotoxicants, five known non-immunotoxicants and four compounds for which it was not fully established whether they are immunotoxic or not (Table 2). Known immunotoxicants from new compound classes include the chloropropanol food contaminant 2,3-dichloro-1-propanol (2,3-DCP) (Lu et al, 2013), the biological anti-CD3 (Ishiguro and Xavier 2004;Weetall et al, 2002), the organophosphorus pesticide chlorpyrifos which causes immunotoxicity in T cells and natural killer cells (Dunier et al, 1991;Noworyta-Glowacka et al, 2012), the lectin phytohaemagglutinin (PHA-L) (Mitsuishi et al, 1986) and the poly-brominated flame-retardant tetrabromobisphenol A (TBBPA) (Pullen et al, 2003;Birnbaum and Staskal, 2004). In addition, immunotoxic compounds from classes tested before were included: the insecticides cypermethrin and aldicarb (Jin et al, 2011), and the mycotoxin zearalenone (Abbes et al, 2013).The insecticide aldicarb increased T cell populations in women drinking contaminated water (Mirkin et al, 1990), however, aldicarb was assessed to be non-immunotoxic in mice (Thomas et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Successful validation of genomic biomarkers for human immunotoxicity in Jurkat T cells in vitro

Schmeits

Shao

Krieken

et al. 2014

J of Applied Toxicology

View full text Add to dashboard Cite

Previously, we identified 25 classifier genes that were able to assess immunotoxicity using human Jurkat T cells. The present study aimed to validate these classifiers. For that purpose, Jurkat cells were exposed for 6 h to subcytotoxic doses of nine immunotoxicants, five non-immunotoxicants and four compounds for which human immunotoxicity has not yet been fully established. RNA was isolated and subjected to Fluidigm quantitative real time (qRT)-PCR analysis. The sensitivity, specificity and accuracy of the screening assay as based on the nine immunotoxicants and five non-immunotoxicants used in this study were 100%, 80% and 93%, respectively, which is better than the performance in our previous study. Only one compound was classified as false positive (benzo-e-pyrene). Of the four potential (non-)immunotoxicants, chlorantraniliprole and Hidrasec were classified immunotoxic and Sunset yellow and imidacloprid as non-immunotoxic. ToxPi analysis of the PCR data provided insight in the molecular pathways that were affected by the compounds. The immunotoxicants 2,3-dichloro-propanol and cypermethrin, although structurally different, affected protein metabolism and cholesterol biosynthesis and transport. In addition, four compounds, i.e. chlorpyrifos, aldicarb, benzo-e-pyrene and anti-CD3, affected genes in cholesterol metabolism and transport, protein metabolism and transcription regulation. qRT-PCR on eight additional genes coding for similar processes as defined in ToxPi analyzes, supported these results. In conclusion, the 25 immunotoxic classifiers performed very well in a screening with new non-immunotoxic and immunotoxic compounds. Therefore, the Jurkat screening assay has great promise to be applied within a tiered approach for animal free testing of human immunotoxicity.

show abstract

Section: Discussioncontrasting

confidence: 79%

Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Successful validation of genomic biomarkers for human immunotoxicity in Jurkat T cells in vitro

Schmeits

Shao

Krieken

et al. 2014

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…Known immunotoxicants from new compound classes include the chloropropanol food contaminant 2,3-dichloro-1-propanol (2,3-DCP) (Lu, et al 2013), the biological anti-CD3 (Ishiguro andXavier 2004, Weetall, et al 2002), the organophosphorus pesticide chlorpyrifos which causes immunotoxicity in T cells and NK cells (Dunier, et al 1991, Noworyta-Glowacka, et al 2012, the lectin phytohaemagglutinin (PHA-L) (Mitsuishi, et al 1986) and the poly-brominated flame retardant tetrabromobisphenol A (TBBPA) (Birnbaum andStaskal 2004, Pullen, et al 2003). In addition, immunotoxic compounds from classes tested before were included: the insecticides cypermethrin and…”

Section: Introductionmentioning

confidence: 99%

Application of omics to immunotoxicology : from mechanisms of action to alternative testing strategies

Shao¹

View full text Add to dashboard Cite

People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal.If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:

show abstract

“…In total we selected nine known immunotoxicants, five known non-immunotoxicants and four compounds for which it was not fully established whether they are immunotoxic or not (Table 2). Known immunotoxicants from new compound classes include the chloropropanol food contaminant 2,3-dichloro-1-propanol (2,3-DCP) (Lu, et al 2013), the biological anti-CD3 (Ishiguro andXavier 2004, Weetall, et al 2002), the organophosphorus pesticide chlorpyrifos which causes immunotoxicity in T cells and NK cells (Dunier, et al 1991, Noworyta-Glowacka, et al 2012, the lectin phytohaemagglutinin (PHA-L) (Mitsuishi, et al 1986) and the poly-brominated flame retardant tetrabromobisphenol A (TBBPA) (Birnbaum andStaskal 2004, Pullen, et al 2003). In addition, immunotoxic compounds from classes tested before were included: the insecticides cypermethrin and aldicarb (Jin, et al 2011), and the mycotoxin zearalenone (Abbes, et al 2013).The insecticide aldicarb increased T cell populations in women drinking contaminated water (Mirkin, et al 1990), however aldicarb was assessed to be non-immunotoxic in mice (Thomas, et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro immunotoxicity tests using transcriptomics

Schmeits¹

View full text Add to dashboard Cite

show abstract

The inhibition of 2,3-dichloro-1-propanol on T cell in vitro and in vivo

Cited by 6 publications

References 32 publications

Successful validation of genomic biomarkers for human immunotoxicity in Jurkat T cells in vitro

Successful validation of genomic biomarkers for human immunotoxicity in Jurkat T cells in vitro

Application of omics to immunotoxicology : from mechanisms of action to alternative testing strategies

Evaluation of in vitro immunotoxicity tests using transcriptomics

Contact Info

Product

Resources

About