The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on <i>C. albicans</i> ATCC 10231

Hamzah, Hasyrul; Hertiani, Triana; Pratiwi, Sylvia Utami Tunjung; Murti, Yosi Bayu; Nuryastuti, Titik

doi:10.5958/0974-360x.2020.00075.x

Cited by 11 publications

(6 citation statements)

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“…[42][43][44][45] Differences in the IC 50 values of extracts and fractions of both leaves are in accordance with the difference in the TLC profiles following the DPPH spraying, which confirmed different chemical contents of both leaves. 46,47 The DPPH radical scavenging activity is classified into 5 groups, i.e., highly active (<50 µg/ml), active (50-100 µg/ml), moderate (101-250 µg/ml), weak (250-500 µg/ml) and inactive (>500 µg/ml). 48 Based on the aforementioned classification, only the water fraction of L. decumana showed an active for radical scavenging activity, while crude extract, hexane and ethyl acetate fraction of L. decumana and the water fraction of L. stimulans exhibited moderate activity, while others are classified as inactive or weak.…”

Section: Samplementioning

confidence: 99%

Uncover Itchy Leaves Ethnomedicine Usage: A Preliminary Study on Characterization and Bioactivity of Laportea Spp

Basy¹,

Santosa²,

Murwanti³

et al. 2022

Pharmacogn. J.

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MATERIALS AND METHODS MaterialsMaterials include the following: Cox-2 (human) inhibitor screening assay kit (Cayman Chemical); n-hexane, ethyl acetate, methanol, formic acid, 96% ethanol, all solvents are pro analyses grade (Merck, Darmstadt, Germany); distilled water; DPPH radical (2,2-diphenyl-1-phycrilhydrazyl) (Sigma Adrich); standard used for the TLC are quercetin, gallic acid, quinine, stigmasterol (Merck, Germany); spraying reagents used are FeCl 3 , AlCl 3 , Dragendorff, annisaldehyde, H 2 SO 4 (Merck, Germany). EquipmentsLinomat  (Camag Linomat 5), Chamber (Durant), Microscope binocular XSG, Spectrophotometer (Geneys 10 UV Scanning, 335903).

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Section: Samplementioning

confidence: 99%

Uncover Itchy Leaves Ethnomedicine Usage: A Preliminary Study on Characterization and Bioactivity of Laportea Spp

Basy¹,

Santosa²,

Murwanti³

et al. 2022

Pharmacogn. J.

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“…Patogenisitas spesies Candida dikaitkan dengan virulensi tertentu faktor, seperti kemampuan untuk menghindari pertahanan inang, adhesi dan pembentukan biofilm (pada jaringan inang atau pada perangkat medis), dan produksi enzim hidrolitik yang merusak jaringan, seperti protease, fosfolipase dan hemolisin Hamzah et al, 2020). Saat ini biofilm diakui sebagai salah mediator utama infeksi dengan perkiraan 80% kejadian infeksi berkaitan dengan pembentukan biofilm (Archer et al, 2011).…”

Section: Pendahuluanunclassified

Aktivitas Ekstrak Etanol Daun Calincing terhadap Biofilm Candida Albicans

Hamzah¹,

Rasdianah

Nurwijayanto³

et al. 2021

farmasetis

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Daun calincing (Oxalis corniculata L.) merupakan salah satu tanaman yang tumbuh di indonesia yang memiliki aktivitas antijamur namun aktivitas antibiofilmnya terhadap C. albicans ATCC 10231 belum pernah dilaporkan. Pencarian antibiofilm baru terhadap biofilm C. albicans menjadi hal penting dalam mencegah infeksi yang berhubungan dengan biofilm. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun calincing (Oxalis corniculata L.) dalam menghambat pembentukan biofilm C. albicans. Pengujian planktonik dan penghambatan biofilm dilakukan dengan menggunakan metode microtiter broth. Aktivitas antibiofilm ekstrak etanol daun calincing terhadap C. albicans. dianalisis dengan menghitung minimum biofilm inhibitor konsentrasi (MBIC50). Ekstrak etanol daun calincing 1 % memberikan aktivitas antijamur C. albicans sebesar 81,23 % ± 0,01 dan aktivitas antibiofilm fase pertengahan sebesar 76,00 % ± 0,01 dan fase pematangan 68,23 ± 0,01. Oleh karena itu, ekstrak etanol daun calincing berpotensi untuk dikembangkan sebagai kandidat obat-obat antibiofilm baru terhadap biofilm C. albicans.

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“…The expression of the target gene was relativized to the gamma tubulin gene 4 (TUB4), and the method used was the comparative "Cq algorithm" (Cq), and expression relative to a calibrator or reference sample by calculating the parameter 2Cq; for which we follow the Bustin´s MIQE guidelines published in 2009 [27]. The ATCC 22019 strain of C. parapsilosis sensu stricto, defined as non-pathogenic by CLSI [28], was used as a reference sample (basal expression) in the analysis of the differential expression of the CPH2 gene in each of the included samples. Three biological replicates were tested for each clinical condition, and in turn each sample was tested in triplicate, in three independent experiments.…”

Section: Measurement Of Gene Expression By Rt-qpcr Applied To the Cph2 Master Gene Depending On The Clinical Origin Of The Isolatesmentioning

confidence: 99%

Oral dysbiosis exacerbates the virulence of Candida parapsilosis sensu stricto via up-regulation of the CPH2 biofilm master gene

Lourdes¹,

Cristina²,

Lorena³

et al. 2021

World J. Adv. Res. Rev.

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Candida parapsilosis sensu stricto is the second to third most frequent cause of candidemia. Studies place this yeast as a frequent colonizer of niches of the oral cavity, predominantly in pathological conditions. We hypothesize that a buccal environment in dysbiosis enhances the virulence of C. parapsilosis sensu stricto. Objective: To evaluate at the phenotype and molecular level the production of biofilm in oral isolates of C. parapsilosis sensu stricto and correlate the results with the clinical origin (dysbiosis versus eubiosis). Material and methods: The biofilm-forming ability was compared in 50 oral isolates of C. parapsilosis sensu stricto obtained from patients with and without oral dysbiosis; by quantification of biofilm biomass and metabolic activity. The results were corroborated by optical and confocal fluorescence microscopy, and correlated with the transcriptional activity of CPH2, by RT-qPCR. The data were analyzed by Excel 2010, and InfoStat 2018, with a 95% confidence interval. Results: The metabolic activity in biofilm was significantly higher in oral dysbiosis relative to control (p = 0.0025). Basal expression of CPH2 increased 2.8 times more in oral dysbiosis related to the control condition and showed no significant differences with pathogenic isolates of this same yeast, derived from onychomycosis lesions. Conclusion: The oral cavity in dysbiosis increases the virulence of C. parapsilosis sensu stricto due to possible changes in epigenetic marks. This finding suggests that the oral cavity in dysbiosis may be an alternative route to the skin in the epidemiology of nosocomial candidemia.

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The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on C. albicans ATCC 10231

Cited by 11 publications

References 0 publications

Uncover Itchy Leaves Ethnomedicine Usage: A Preliminary Study on Characterization and Bioactivity of Laportea Spp

Uncover Itchy Leaves Ethnomedicine Usage: A Preliminary Study on Characterization and Bioactivity of Laportea Spp

Aktivitas Ekstrak Etanol Daun Calincing terhadap Biofilm Candida Albicans

Oral dysbiosis exacerbates the virulence of Candida parapsilosis sensu stricto via up-regulation of the CPH2 biofilm master gene

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