The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring

Abstract

AFLP T M is a new technique to generate large numbers of molecular markers for genetic mapping. The method involves the selective amplification of a limited number of DNA restriction fragments out of complex plant genomic DNA digests using PCR. With six primer combinations 264 segregating AFLP amplification products were identified in a diploid backcross population from non-inbred potato parents. The identity of an AFLP marker was specified by the primer combination of the amplification product and its size estimated in bases. The segregating AFLP amplification products were mapped by using a mapping population with 217 already known RFLP, isozyme and morphological trait loci. in general, the AFLP markers were randomly distributed over the genome, although a few clusters were obselwed. No indications were found that AFLP markers are present in other parts of the genome than those already covered by RFLP markers. Locus specificity of AFLP markers was demonstrated because equally sized amplification products segregating from both parental clones generally mapped to indistinguishable maternal and paternal map positions. Locus specificity of AFLP amplification products will allow to establish the chromosomal identity of linkage groups in future mapping studies.Since AFLP technology is a multi-locus detection system, it was not possible to identi~ the AFLP alleles which belong to a single AFLP locus. The consequences of a genetic analysis based on single alleles, rather than on loci with two or more alleles on mapping studies using progenies of non-inbred parents are discussed.

Molecular Genetic Maps in Wild Emmer Wheat, Triticum dicoccoides: Genome-Wide Coverage, Massive Negative Interference, and Putative Quasi-Linkage

“…A great advantage of the AFLP approach is that it allows simultaneous identification of a large number of amplification products (Van Eck et al 1995;Vos et al 1995). Compared with RFLP or other PCR-based marker systems, AFLP is fast, reliable, and costeffective.…”

A limited set of starch related genes explain several interrelated traits in potato

“…The integrated map of the C 9 E population consisting of 192 dominantly scored AFLP markers, 28 SSR markers and 24 CAPS/SCAR markers calculated as done previously (van Eck et al 1995). The linkage groups were assigned to the 12 linkage groups representing the 12 chromosomes on the basis of the earlier version of this map comprising a total length of 996 cm.…”

“…In this study an expanded version of the linkage map of van Eck et al (1995) was used, with an offspring size expanded to 249 full sib descendants. All additional individuals were genotyped using 16 AFLP markers with 15 primer combinations E32/ M47, E32/M48, E32/M49, E32/M50, E32/M51, E32/ M54, E32/M59, E32/M61, E35/M47, E35/M48, E35/ M50, E38/M59, E39/M60, E45/M59 and E45/M60.…”

Laboratory Typing Methods for Diagnostic of Salmonella Strains, the “Old” Organism That Continued Challenges

“…These characteristics make AFLP a powerful fingerprinting technique which can be used in identification, epidemiology and taxonomy (Folkerstma et al 1996;Huys et al 1996;Janssen et al 1996). In addition, the technique can be used to generate large numbers of molecular markers for linkage studies (Ballvora et al 1995;Becker et al 1995;van Eck et al 1995).…”