The Influence of α-Tocopherol on Serum Biochemical Markers During Experimentally Induced Pleuritis in Rats Exposed to Dioxin

Abstract

Toxicity of dioxins is wide ranging. Amongst the organs, the liver is the most susceptible to damage by dioxins. Damage caused to liver cells results in promoting inflammatory processes. The aim of this work was to evaluate whether high doses of tocopherol will change the inflammatory response, monitored by biochemical indicators, by improving liver function in rats exposed to tetrachlorodibenzo-p-dioxin (TCDD). The study was conducted on a population of female Buffalo rats. The animals were divided into the following groups: Control Group A—representing physiological norms for the studied diagnostic indicators; Control Group B—subjects were administered a 1% ceragenin solution to induce pleuritis; Study Group 1—where rats were administered α-tocopherol acetate for 3 weeks, after which pleuritis was induced; Study Group 2—rats were administered a single dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), while 3 weeks later, pleuritis was induced; and Study Group 3—rats were administered a single dose of TCDD and next, were administered α-tocopherol acetate for 3 weeks, followed by pleuritis induction. The results clearly show that administering tocopherol in the course of inflammation causes changes to the distribution and ratio of in the serum protein fractions, including acute phase proteins. The latter proteins are indicative to the improvement in liver function and linked to protein synthesis and stimulation of the antibody-mediated immunity. Moreover, in the course of inflammation caused by exposure of rats to TCDD, tocopherol significantly affected the acute phase protein concentration.

Dynamic Analysis of Changes of Protein Levels and Selected Biochemical Indices in Rat Serum in the Course of Experimental Pleurisy

“…The initiated inflammatory process and the release of hormones accompanying this phenomenon impact liver metabolism, which itself impacts coagulation processes, activation of the complement system, and changes in the concentrations of metals such as Fe, Cu, and Zn. The latter are associated with increased synthesis of acute-phase proteins, which play a protective role in the management of these metals [ 7 , 8 ].…”

Tibial Cortex Transverse Transport Facilitates Severe Diabetic Foot Wound Healing via HIF-1α-Induced Angiogenesis

“…The animals were anesthetized with 35 mg/kg of 2% sodium pentobarbital intraperitoneally. 42 First, the femoral artery was ligated to induce hindlimb ischemia ( Figure 2A ). Briefly, a 1-cm incision was made where the strongest pulsation of the femoral artery was palpable, 43 , 44 then the subcutaneous fascia was retracted to expose the femoral artery.…”

“…The animals were anesthetized with 35 mg/kg of 2% sodium pentobarbital intraperitoneally. 42 First, the femoral artery was ligated to induce hindlimb ischemia Figure 1 This schema shows the study design and the assessments at each stage. In the control group, surgery including induction of hindlimb ischemia and creation of DFU was performed 4 weeks after diabetes induction.…”