The influence of tumour cell DNA content on survival in colorectal cancer: a detailed analysis

Flow cytometric analysis of ploidy in colorectal cancer: a multicentric experience

“…Conversely, our data are in disagreement with most reported results of several studies with a longer followup (Armitage et al, 1990;Harlow et al, 1991;Rognum et al, 1991). Such results appear to indicate that DNA ploidy could be a late indicator of survival, and this hypothesis is supported by the appearance of a prognostic relevance of DNA ploidy at 5 years (Rognum et al, 1991) in the same series of patients in which clinical relevance had not been detected at a shorter follow-up (Rognum et al, 1987).…”

“…In particular, FCM analysis has been extensively used in colorectal cancer to evaluate ploidy (Bauer et al, 1987;Kouri et al, 1990;Quirke et al, 1987;Schutte et al, 1987). Different frequencies of aneuploid tumours and conflicting information on the prognostic relevance of ploidy have been reported by different studies (Armitage et al, 1990;Jass et al, 1989;Jones et al, 1988;Kokal et al, 1986;Kouri et al, 1990;Quirke et al, 1987) and can be ascribed to technical and biological reasons. One of the most crucial points is the type of material used for FCM analysis.…”

Stage-specific frequency and prognostic significance of aneuploidy in patients with sporadic colorectal cancer—a meta-analysis and current overview

“…Overall, only 21 studies including 5203 samples also analyzed survival rates in individual tumor stages with regard to [38] 1990 416 FC n.a. (>60) 0.02 a n.s.…”

Numerical chromosome alterations in colorectal carcinomas detected by fluorescence in situ hybridization

“…Several investigations have shown that D N A aneuploidy is associated with aggressive behaviour of colorectal carcinomas, and it has been p r o p o s e d that this abnormality is a good parameter for indicating a poor prognosis [1,10,18]. Both D N A aneup l o i d y and molecular genetic alterations represent genetic changes of cancer cells, albeit at different levels, and For example, genetic alterations appear to be linked to chromosomal instability and to the rather frequent occurrence of chromosomal aberrations [10].…”

“…In addition, flowcytometric [1,10,18,19] and image analysis [2,[5][6][7]42] studies have shown that alterations in the DNA content of cancer cells reflect the aggressiveness of a given tumour. Recent advances in molecular biology have established that a series of molecular events is involved in carcinogenesis, and these studies have led to the discovery of numerous oncogenes and tumour suppressor genes.…”