The influence of topographic microstructures on the initial adhesion of L929 fibroblasts studied by single-cell force spectroscopy

Elter, Patrick; Weihe, Thomas; Lange, Regina; Gimsa, Jan; Beck, Ulrich

doi:10.1007/s00249-010-0649-0

Cited by 27 publications

(22 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, our findings on CS (0.17-2.08 nN/lm) are in corroboration with their data ($0.2 -$1.8 nN/lm). In another study concerning the adhesion of murine L929 fibroblasts to microstructured titanium, 26 detachment forces of the same force range ($3 nN) were revealed after 1 min of interaction between cells and surfaces. Even though the cell line, the interaction time, and sample preparation differed in our study, these comparisons show that the range of detachment forces after less than 1 min of cell-substrate-interaction should lie in an interval between some hundred of piconewtons and a few nanonewtons.…”

Section: Discussionmentioning

confidence: 80%

“…Chemical and physical surface properties such as texture, roughness, and porosity determine the extent of FBR 18–22. It has been shown that cells strongly respond to nano‐ and microstructures 23–30. However, the underlying mechanisms are not fully understood 31.…”

Section: Introductionmentioning

confidence: 99%

“…[18][19][20][21][22] It has been shown that cells strongly respond to nano-and microstructures. [23][24][25][26][27][28][29][30] However, the underlying mechanisms are not fully understood. 31 To prevent cells from adhering to material surfaces, antiwetting surface modifications could be promising.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of single‐cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond‐laser microstructured titanium surfaces

Aliuos

Fadeeva

Badar

et al. 2012

J Biomedical Materials Res

View full text Add to dashboard Cite

One goal in biomaterials research is to limit the formation of connective tissue around the implant. Antiwetting surfaces are known to reduce ability of cells to adhere. Such surfaces can be achieved by special surface structures (lotus effect). Aim of the study was to investigate the feasibility for creating antiwetting surface structures on titanium and to characterize their effect on initial cell adhesion and proliferation. Titanium microstructures were generated using femtosecond- (fs-) laser pulses. Murine fibroblasts served as a model for connective tissue cells. Quantitative investigation of initial cell adhesion was performed using atomic force microscopy. Fluorescence microscopy was used for the characterization of cell-adhesion pattern, cell morphology, and proliferation. Water contact angle (WCA) measurements evinced antiwetting properties of laser-structured surfaces. However, the WCA was decreased in serum-containing medium. Initial cell adhesion to microstructured titanium was significantly promoted when compared with polished titanium. Microstructures did not influence cell proliferation on titanium surfaces. However, on titanium microstructures, cells showed a flattened morphology, and the cell orientation was biased according to the surface topography. In conclusion, antiwetting properties of surfaces were absent in the presence of serum and did not hinder adhesion and proliferation of NIH 3T3 fibroblasts.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of single‐cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond‐laser microstructured titanium surfaces

Aliuos

Fadeeva

Badar

et al. 2012

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…The adhesion phenomenon, characterized as negative force in the experimental force-displacement curves obtained in AFM nanoindentation, was widely reported over the last two decades [10][11][12][13]. The adhesion behavior of cells with other nanoparticles is crucial for the biocompatibility of implants [14]. In recent years, it becomes clear that adhesion molecules are involved in tethering cells to specific locations [15].…”

Section: Introductionmentioning

confidence: 99%

Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nano-indentation

Zhu

Liu

Zhang

et al. 2015

2015 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3m-Nano)

View full text Add to dashboard Cite

In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young's modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment.

show abstract

“…Furthermore, interactions between the cells and the extracellular matrix (ECM) contribute in the maintenance of the structural integrity of tissues 21 . In addition, the adhesive behaviour of cell with other surfaces is crucial for the biocompatibility of implants 7 . Cell adhesion is a dynamic process, which is controlled by the binding of adhesion molecules on the cell surface 23 .…”

Section: Introductionmentioning

confidence: 99%

Nanomechanical Investigation of Soft Biological Cell Adhesion using Atomic Force Microscopy

et al. 2014

View full text Add to dashboard Cite

Mechanical coupling between living cells is a complex process that is important for a variety of biological processes. In this study the effects of specific biochemical treatment on cell-to-cell adhesion and single cell mechanics were systematically investigated using Atomic Force Microscopy (AFM) Single Cell Force Spectroscopy. Functionalised AFM tipless cantilevers were used for attaching single suspended cells that were brought in contact with substrate cells. Cell-to-cell adhesion parameters, such as maximum unbinding force (F max ) and work or energy of detachment (W D ), were extracted from the retraction force-displacement (F-d) curves. AFM indentation experiments were performed by indenting single cells with a spherical microbead attached to the cantilever. Hertzian contact model was applied to determine the elastic modulus (E). Following treatment of the cells with neutralising antibody for epithelial (E)-cadherin, F max was increased by 25%, whereas W D decreased by 11% in response to a 43% increase in E. The results suggest that although the adhesion force between cells increased after treatment due to higher ligand-receptor binding, the energy of adhesion was decreased due to the reduced displacement separation as manifested by the loss of elastic deformation. Conclusively, changes in single cell mechanics are important underlying factors contributing to cell-to-cell adhesion and hence cytomechanical characterization is critical for cell adhesion measurements.

show abstract

The influence of topographic microstructures on the initial adhesion of L929 fibroblasts studied by single-cell force spectroscopy

Cited by 27 publications

References 47 publications

Evaluation of single‐cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond‐laser microstructured titanium surfaces

Evaluation of single‐cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond‐laser microstructured titanium surfaces

Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nano-indentation

Nanomechanical Investigation of Soft Biological Cell Adhesion using Atomic Force Microscopy

Contact Info

Product

Resources

About