The influence of tissue section thickness on the study of collagen by the Picrosirius-polarization method

Junqueira, L. C.; Montes, G. S.; Sánchez, E.

doi:10.1007/bf00495061

Cited by 199 publications

(131 citation statements)

References 12 publications

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“…Studies using the technique of staining with picrosirius when analyzed by polarized light microscope, analyze collagen using birefringence, wherein the collagen type I is evident in color from yellow to red, while type III collagen fibers are shown as greenish and the remainder of the cell is presented as black (Franca et al, 2013;Junqueira et al, 1982;Rodrigues et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

et al. 2015

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SUMMARY:The aim of this study was to analyze changes in the skeletal muscle tissue of rats after muscle stretch injury using fractal analysis. Nineteen rats were divided into three groups, Control (n= 5) (C), Lesion two days (n= 7) (L2), and Lesion seven days (n= 7) (L7). Histological slides of the gastrocnemius muscle of the animals were prepared and stained with Hematoxylin and eosin for analysis of the inflammatory process, and picrosirius for the demonstration of collagen. The stained slides were subsequently analyzed using Fractal Dimension (FD). The results showed high FD values of the inflammatory process in the experimental groups L2 and L7 in relation to C (p= 0.000). The analysis of collagen in the picrosirius stained slides showed high FD in the L2 group compared to the L7 (p= 0.0001), with no statistically significant difference among other groups. In summary, the present study demonstrated that fractal analysis was able to identify changes in the inflammatory process in injured skeletal muscle tissue.

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Section: Discussionmentioning

confidence: 99%

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

et al. 2015

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“…For quantification of collagen fibers the sections stained by Picro-Sirius Red technique were observed under polarized light (13). The analyzed images were obtained with a magnification of x400 using a video camera coupled to a light microscope.…”

Section: Methodsmentioning

confidence: 99%

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

2007

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Objective: Compare detrusor muscle of normal and patients with infravesical obstruction, quantifying the collagen and elastic system fibers. Materials and Methods:We studied samples taken from bladders of 10 patients whose ages ranged from 45 to 75 years (mean = 60 years), who underwent transvesical prostatectomy for treatment of BPH. Control material was composed of 10 vesical specimens, removed during autopsies performed in cadavers of accident victims, with ages between 18 and 35 years (mean = 26 years). Results:The results of collagen and elastic fibers quantification (volumetric density) demonstrated the following results in percentage (mean +/-standard deviation): collagen in BPH patients = 4.89 +/-2.64 and 2.32 +/-1.25 in controls (p < 0.0001), elastin in BPH patients = 10.63% +/-2.00 and 8.94% +/-1.19 in controls (p < 0.0001). Conclusion:We found that the components of connective tissue, collagen and elastic system fibers are increased in the detrusor muscle of patients with infravesical obstruction, when compared to controls.

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“…O método de Picrosirius, juntamente com a microscopia de polarização, demonstra com mais nitidez as fibras colágenas. [21][22][23][24][25] No trabalho observou-se que a quantidade de colágeno das pregas vocais submetidas aos diferentes tipos de cirurgia, tanto com laser de CO 2 quanto com bisturi com lâmina a frio, apresentava diferença significantemente estatística, como observado nas Tabelas 1 e 2. A presença de colágeno foi significantemente maior nas pregas vocais submetidas a incisões com bisturi com lâmina a frio do que com laser de CO 2 , o que permite concluir que o impacto causado na produção da voz é menor em pacientes submetidos a cirurgia com laser quando utilizados baixa potência e um microspot pequeno.…”

Section: Discussionunclassified

Estudo comparativo histológico na prega vocal após incisão com instrumental a frio e com laser de CO2 em modelo animal

Santos¹,

Grellet

Aguilar

et al. 2003

Rev. Bras. Otorrinolaringol.

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Dent re os vários tipos de laser usados em Medicina, o laser de CO 2 é o mais usado na Otorrinolaringologia e Cirurgia de Cabeça e Pescoço. As vantagens de seu uso são a diminuição do sangramento, a diminuição do edema no pós-operatório e a facilidade de acesso ao campo operatório, entre outras. Desde os trabalhos de Jako e Strong em 1972 1,2 , quando o laser de CO 2 passou a ser usado no tratamento de papilomatose laríngea e de lesões malignas glóticas iniciais, suas indicações têm aumetado, principalmente em lesões benignas, a partir da alta tecnologia desenvolvida dos últimos anos como, por exemplo, a diminuição do microspot e o uso do superpulso, reduzindo conseqüentemente seu efeito térmico sobre os tecidos. Métodos: Neste trabalho foram realizadas incisões com instrumental a frio e com laser de CO 2 1 watt de modo contínuo e superpulso, em pregas vocais caninas e observado, através de cortes histológicos corados pelo método de Sirius Red, a quantidade de colágeno depositada sobre as mesmas. Resultados: A quantidade de colágeno das pregas vocais foi maior do que no grupo controle, e estatisticamente maior no grupo de animais submetidos a procedimentos com instrumental a frio do que com laser de CO 2 . Não houve diferença estatística entre o grupo controle e o grupo submetido a incisões com instrumentos a frio. Conclusão: A microcirurgia de laringe com o laser de CO 2 , quando este é usado em baixa potência, com pequeno "microspot" e com superpulso, é um método seguro em relação à deposição de colágeno, quando comparado com instrumentos com lâmina a frio, obedecendo os princípios da fonomicrocirurgia. Among the several types of lasers employed in medicine today, the CO 2 is the most widely used in otorhinolaryngology and head and neck surgeries. The advantages of its use are: the reduction in bleeding, reduction in post-operative edema and the easy access to the operation site, among others. Ever since Jako and Strong's works in 1972 1,2 , when CO 2 was first applied in the treatment of papilomatosis and early glottic malign lesions, its indications have risen, most especially in benign lesions, due to new technology developed over the last years as, for example, the reduction of microspot and the superpulse, reducing the thermal effects on the tissues. Methods: In this work, incisions with cold steel instruments and 1watt continuous mode and super-pulse CO 2 laser were conducted on canine vocal folds. The amount of collagen deposited over the vocal folds was observed through histological exams using the Sirius Red method. Results: The amount of collagen on the vocal folds was greater than the control group and statistically greater in the group of animals submitted to surgical procedures using cold instruments than procedures involving CO 2 . There was no statistical difference between the control group and the group submitted to incisions with cold steel instruments. Conclusions:The larynx surgery with CO 2 laser, when employed in low potency, with small microspot and superpulse, is a safe method in relati...

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The influence of tissue section thickness on the study of collagen by the Picrosirius-polarization method

Cited by 199 publications

References 12 publications

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

Estudo comparativo histológico na prega vocal após incisão com instrumental a frio e com laser de CO2 em modelo animal

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