Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

Ozaki, Guilherme Akio Tamura; Camargo, Regina Celi Trindade; Koike, Tatiana Emy; Garcia, Thiago Alves; Castoldi, Robson Chacon; Filho, José Carlos Silva Camargo

doi:10.4067/s0717-95022015000300017

Cited by 13 publications

(14 citation statements)

References 21 publications

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“…In this way FD has been successfully used to identify cancer cells, discriminate the architecture of muscles with dystrophy as well as differentiate brain cells (Lee et al, 2014;Pantic et al, 2015Pantic et al, , 2014Cury et al, 2018), and these processes are linked to the number of nuclei in the analyzed site. In another study from our group (Ozaki et al, 2015), FD proved to be effective in differentiating injured muscle from healthy muscle, a fact that was again observed in the present study. Although, the box-counting method founded differences in FD of a healthy muscle to an injured in histological aspects, but it was not sensitive to differentiate two distinct treatments or to find differences in collagen levels.…”

Section: Discussionsupporting

confidence: 88%

Fractal dimension in the evaluation of different treatments of muscular injury in rats

et al. 2018

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Objectives: To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method. Methods: 35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method. Results: In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups. Conclusion: Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.

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Section: Discussionsupporting

confidence: 88%

Fractal dimension in the evaluation of different treatments of muscular injury in rats

et al. 2018

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“…In the fractal dimension when we submitted HE-stained blades to the binarization process, the nuclei are detached from the rest of the cells, so it is possible to measure the size of the infiltrate or other findings by the analysis of its fractal dimension [25] The findings of this study show that DF is a tool that can be used as a means of quantitative analysis of a lesion, which adds to the study an independent evaluation of the evaluator. Although there were not many significant statistical differences in the comparison between the groups, the fractal dimension analysis showed that animals poisoned by venom and even those submitted to treatment with antiofidic serum showed an increase in the fractal dimension in comparison to CG animals, suggesting that they have a larger fractal dimension.…”

Section: Discussionmentioning

confidence: 87%

“…However it is known that this type of analysis is dependent on the analyzer, which leads to intra and interobserver variations [21]. Unlike this method, fractal analysis, in addition to quantifying histological changes, is independent of the evaluator, since it is an automatic analysis performed by software [25].…”

Section: Discussionmentioning

confidence: 99%

Fractal Dimension in Liver Histological Findings of Wistar Rats Experimentally Intoxicated with Venom of Crotalus durissus terrificus

Santos

Goiozo

Brito

et al. 2019

Acta Scientiae. Vet.

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Background: Accidents caused by venom of Crotalus durissus snakes, popularly known in Brazil as rattlesnake, are second in relation to the occurrence and first place in deaths in humans and animals, mainly due to the great neurotoxic, myotoxic, coagulant, nephrotoxic and hepatotoxic potential of their venom. The effects observed are due to the action of the main poison fractions and among them we can mention crotoxin (representing 50% of the total poison), crotamine, gyroxine and conxulxin. The present study aimed to analyze the liver of experimentally poisoned Wistar rats with venom of Crotalus durissus terrificus by means of histological and fractal analysis. The hypothesis is that the venom of Crotalus durissus terrificus is can induce hepatic damage at the dose recommended in this study, that its alterations can be quantified by the fractal dimension and that the antiofidic serum botropic crotalic be able to minimize the hepatic lesions induced by the venom. Materials, Methods & Results: Ninety rats were distributed into different groups and treated with: control group (GC, n = 30) 0.9% sodium chloride solution; venom group (GV, n = 30) crotalic venom at the dose of 1 mg/kg; (GVS, n = 30) crotalic venom at the dose of 1 mg/Kg and antiofidic serum 6 h after the application of the venom at the dose recommended by the manufacturer. Liver samples were collected at 2 h (M1), 8 h (M2) and 24 h (M3) after venom administration and submitted to histological analysis and fractal dimension (DF) using the ImageJ® software and box-counting method. Procedures for collecting, processing and analyzing samples were standardized. For statistical analyzes, after the normality was verified by the Shapiro-Wilk test and homogeneity by the Bartlett test, the data were submitted to analysis of variance (ANOVA) with Duncan test contrast with a significance level of 5%. No significant lesions were observed in GC, however necrosis, cytoplasmic and nuclear vacuolization and absence of inflammatory infiltrate were observed in 8 h (M2) and 24 h (M3) in GV. In addition to the lesions found in the GV, mononuclear inflammatory infiltrate was evident always (2 h, 8 h and 24 h) in the GVS. The lesions of necrosis, cytoplasmic and nuclear vacuolization, considered of greater severity were visualized in M3 (24 h) in both GV and GVS. There was an increase in DF for the same changes in GV and GVS over time, but with no difference between them, but with a significant difference compared to CG. In this study, lesions evidenced in the liver were not minimized by the application of antiofidic serum at the recommended dose and during the time of observation of the animals. Discussion: This study agrees with other authors about the hepatotoxicity of crotalic venom in relation to histological findings and the results indicate an increase in DF for the findings of vacuolization and necrosis, proving to be an efficient method for the quantitative evaluation of morphological changes induced by venom without observer interference. In addition, non-protection...

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“…Cuts measuring 5 μm in thickness were made in a cryostat microtome at −20 °C, placed on slides and stained with hematoxylin-eosin (HE) for analysis of the structure of the muscles, and Picrosirius red for collagen analysis. 20 , 21 …”

Section: Methodsmentioning

confidence: 99%

Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

Garcia

Camargo

Koike

et al. 2017

Brazilian Journal of Physical Therapy

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Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury

Cited by 13 publications

References 21 publications

Fractal dimension in the evaluation of different treatments of muscular injury in rats

Fractal dimension in the evaluation of different treatments of muscular injury in rats

Fractal Dimension in Liver Histological Findings of Wistar Rats Experimentally Intoxicated with Venom of Crotalus durissus terrificus

Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

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