Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

Garcia, Thiago Alves; Camargo, Regina Celi Trindade; Koike, Tatiana Emy; Ozaki, Guilherme Akio Tamura; Castoldi, Robson Chacon; Filho, José Carlos Silva Camargo

doi:10.1016/j.bjpt.2017.06.007

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…Studies also performed histologic[3,6,8,9,15,17,18,20-25] and gross anatomical[6,15,17,20,21,23] assessments of the muscles (Table 5). Eight studies reported improved functional and structural variables, reduced fibrosis, and increased muscular regeneration in PRP-treated muscle compared to control muscle[6,15,17,20-23,25]. Several studies reported increased angiogenesis after PRP treatment[17,20,21,23].…”

Section: Resultsmentioning

confidence: 99%

“…Several studies reported increased angiogenesis after PRP treatment[17,20,21,23]. Four studies reported increased leukocyte infiltration at the injection site[8,9,18,25]. With regards to changes in collagen deposition, one study reported increased type III collagen, one reported increased type I collagen[25], and two reported no change in collagen content[2,8].…”

Section: Resultsmentioning

confidence: 99%

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Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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BACKGROUND Platelet-rich plasma (PRP) is an increasingly used biologic adjunct for muscle injuries, as it is thought to expedite healing. Despite its widespread use, little is known regarding the mechanisms by which PRP produces its efficacious effects in some patients. AIM To clarify the effects of PRP on muscular pathologies at the cellular and tissue levels by evaluating the basic science literature. METHODS A systematic review of PubMed/MEDLINE and EMBASE databases was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and checklist. Level III in vivo and in vitro studies examining PRP effects on muscles, myocytes and/or myoblasts were eligible for inclusion. Extracted data included PRP preparation methods and study results. RESULTS Twenty-three studies were included (15 in vivo , 6 in vitro , 2 in vitro / in vivo ). Only one reported a complete PRP cytology (platelets, and red and white blood cell counts). Five in vitro studies reported increased cellular proliferation, four reported increased gene expression, and three reported increased cellular differentiation. Five in vivo studies reported increased gene expression, three reported superior muscle regeneration, and seven reported improved histological quality of muscular tissue. CONCLUSION The basic science literature on the use of PRP in muscle pathology demonstrates that PRP treatment confers several potentially beneficial effects on healing in comparison to controls. Future research is needed to determine optimal cytology, dosing, timing, and delivery methods of PRP for muscle pathologies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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show abstract

“…As possible mechanisms, decreased inflammation and muscle creatine kinase levels are discussed. The combination of LLLT with platelet rich plasma (PRP) produced better results for promoting muscle regeneration after injuries compared to the isolated use of LLLT or PRP [ 118 ].…”

Section: Developing Technologies For Muscle Tissue Engineering Andmentioning

confidence: 99%

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

Liu

Saul

Böker

et al. 2018

BioMed Research International

114

139

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Skeletal muscle has the capacity of regeneration after injury. However, for large volumes of muscle loss, this regeneration needs interventional support. Consequently, muscle injury provides an ongoing reconstructive and regenerative challenge in clinical work. To promote muscle repair and regeneration, different strategies have been developed within the last century and especially during the last few decades, including surgical techniques, physical therapy, biomaterials, and muscular tissue engineering as well as cell therapy. Still, there is a great need to develop new methods and materials, which promote skeletal muscle repair and functional regeneration. In this review, we give a comprehensive overview over the epidemiology of muscle tissue loss, highlight current strategies in clinical treatment, and discuss novel methods for muscle regeneration and challenges for their future clinical translation.

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“…In particular, PRP injections into skeletal muscles of rats or mice subjected to different traumatic injuries (incision, laceration, contusion or lengthening/eccentric contractions) or to cardiotoxin injection or into ischemic muscles, have been demonstrated to contribute to the muscle healing process: (i) by modulating the inflammatory response including the increase in M2 macrophage cell recruitment to the injury site and function [105,106,107,108]; (ii) by generating a myogenic response, as evaluated by satellite cell activation, increase of the expression of different myogenic regulatory factors, modulation of the expression of muscle specific microRNAs and activation of myogenic signaling pathways leading to myofiber formation [105,106,109,110,111,112,113]; (iii) by attenuating the impairment of myocytes mitochondrial function determined by muscle damage and improving their endogenous antioxidant defense system [114]; and (iv) by protecting cells from apoptosis [108,111].…”

Section: Plasma Preparations: Platelet-rich Plasma (Prp) and Platementioning

confidence: 99%

Influence of Platelet-Rich and Platelet-Poor Plasma on Endogenous Mechanisms of Skeletal Muscle Repair/Regeneration

Chellini

Tani

Zecchi‐Orlandini

et al. 2019

IJMS

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The morpho-functional recovery of injured skeletal muscle still represents an unmet need. None of the therapeutic options so far adopted have proved to be resolutive. A current scientific challenge remains the identification of effective strategies improving the endogenous skeletal muscle regenerative program. Indeed, skeletal muscle tissue possesses an intrinsic remarkable regenerative capacity in response to injury, mainly thanks to the activity of a population of resident muscle progenitors called satellite cells, largely influenced by the dynamic interplay established with different molecular and cellular components of the surrounding niche/microenvironment. Other myogenic non-satellite cells, residing within muscle or recruited via circulation may contribute to post-natal muscle regeneration. Unfortunately, in the case of extended damage the tissue repair may become aberrant, giving rise to a maladaptive fibrotic scar or adipose tissue infiltration, mainly due to dysregulated activity of different muscle interstitial cells. In this context, plasma preparations, including Platelet-Rich Plasma (PRP) and more recently Platelet-Poor Plasma (PPP), have shown advantages and promising therapeutic perspectives. This review focuses on the contribution of these blood-derived products on repair/regeneration of damaged skeletal muscle, paying particular attention to the potential cellular targets and molecular mechanisms through which these products may exert their beneficial effects.

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Histological analysis of the association of low level laser therapy and platelet-rich plasma in regeneration of muscle injury in rats

Abstract: HighlightsLLLT and PRP can be used to enhance muscle regeneration.The association of LLLT with PRP produces better treatment than their use in isolation.LLLT could modulate the production of collagen during muscle injury.

Cited by 12 publications

References 30 publications

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Current Methods for Skeletal Muscle Tissue Repair and Regeneration

Influence of Platelet-Rich and Platelet-Poor Plasma on Endogenous Mechanisms of Skeletal Muscle Repair/Regeneration

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