The influence of tissue microenvironment on stem cell–based cartilage repair

Jayasuriya, Chathuraka T.; Chen, Yupeng; Liu, Wenguang; Chen, Qian

doi:10.1111/nyas.13170

Cited by 46 publications

(51 citation statements)

References 110 publications

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“…Mesenchymal stem cells (MSCs) represent an attractive cell source for cartilage tissue engineering, thanks to their immunomodulatory property and self‐renewing and high proliferative capability (Jayasuriya, Chen, Liu, & Chen, ; Manferdini et al, ; Maumus et al, ). MSCs have multilineages (adipogenesis, chondrogenesis, and osteogenesis) potential (Dominici et al, ; Mortada & Mortada, ) and, in combination with biomaterials, well support cell attachments and stimulate ECM synthesis (Bernhard & Vunjak‐Novakovic, ).…”

Section: Introductionmentioning

confidence: 99%

“…CH polymer was cross-linked with substances in order to optimize resistance and elasticity (Comblain, Rocasalbas, Gauthier, & Henrotin, 2017). Moreover, it possesses certain intriguing characteristics such as high surface-volume ratio, great porosity on surface, possibility to obtain small pore size, ability to control biodegradation processes, and improved mechanical properties (Rodriguez-Vazquez et al, 2015) Mesenchymal stem cells (MSCs) represent an attractive cell source for cartilage tissue engineering, thanks to their immunomodulatory property and self-renewing and high proliferative capability (Jayasuriya, Chen, Liu, & Chen, 2016;Manferdini et al, 2013;Maumus et al, 2013). MSCs have multilineages (adipogenesis, chondrogenesis, and osteogenesis) potential (Dominici et al, 2006;Mortada & Mortada, 2018) and, in combination with biomaterials, well support cell attachments and stimulate ECM synthesis (Bernhard & Vunjak-Novakovic, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Manferdini

Gabusi

Sartore

et al. 2019

J Tissue Eng Regen Med

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Cartilage tissue engineering remains problematic because no systems are able to induce signals that contribute to native cartilage structure formation. Therefore, we tested the potentiality of gelatin‐polyethylene glycol scaffolds containing three different concentrations of chitosan (CH; 0%, 8%, and 16%) on chondrogenic differentiation of human platelet lysate‐expanded human bone marrow mesenchymal stromal cells (hBM‐MSCs). Typical chondrogenic (SOX9, collagen type 2, and aggrecan), hypertrophic (collagen type 10), and fibrotic (collagen type 1) markers were evaluated at gene and protein level at Days 1, 28, and 48. We demonstrated that 16% CH scaffold had the highest percentage of relaxation with the fastest relaxation rate. In particular, 16% CH scaffold, combined with chondrogenic factor TGFβ3, was more efficient in inducing hBM‐MSCs chondrogenic differentiation compared with 0% or 8% scaffolds. Collagen type 2, SOX9, and aggrecan showed the same expression in all scaffolds, whereas collagen types 10 and 1 markers were efficiently down‐modulated only in 16% CH. We demonstrated that using human platelet lysate chronically during hBM‐MSCs chondrogenic differentiation, the chondrogenic, hypertrophic, and fibrotic markers were significantly decreased. Our data demonstrate that only a high concentration of CH, combined with TGFβ3, creates an environment capable of guiding in vitro hBM‐MSCs towards a phenotypically stable chondrogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Manferdini

Gabusi

Sartore

et al. 2019

J Tissue Eng Regen Med

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“…To date, these objectives are far from being achieved and optimal cartilage reconstruction still represents an unmet clinical need [2,3]. Cell-based therapy for cartilage repair aims at not only filling the tissue defect with a substitute, but also reconstituting the structure, physicochemical properties, and functionality of the hyaline matrix, possibly promoting intimate integration with the resident tissue [4,5]. Ideally, this is achieved by implanting a sufficient number of mature chondrocytes or undifferentiated progenitor cells with a high chondrogenic potential [6].…”

Section: Introductionmentioning

confidence: 99%

Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair

Lolli

Penolazzi

Narcisi

et al. 2017

Cell. Mol. Life Sci.

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The field of cartilage repair has exponentially been growing over the past decade. Here, we discuss the possibility to achieve satisfactory regeneration of articular cartilage by means of human mesenchymal stem cells (hMSCs) depleted of anti-chondrogenic factors and implanted in the site of injury. Different types of molecules including transcription factors, transcriptional co-regulators, secreted proteins, and microRNAs have recently been identified as negative modulators of chondroprogenitor differentiation and chondrocyte function. We review the current knowledge about these molecules as potential targets for gene knockdown strategies using RNA interference (RNAi) tools that allow the specific suppression of gene function. The critical issues regarding the optimization of the gene silencing approach as well as the delivery strategies are discussed. We anticipate that further development of these techniques will lead to the generation of implantable hMSCs with enhanced potential to regenerate articular cartilage damaged by injury, disease, or aging.

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“…CSPCs are multipotent stem cells and identify by expression of specific surface markers, ability to self-renew, and differentiate into three cell types such as adipocytes, osteoblasts, and chondrocytes [37,38]. These cells are great tools for cell-based cartilage repair [37,[39][40][41].…”

Section: Avian Cartilage Stem/progenitor Cellsmentioning

confidence: 99%

Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell-Based Therapies

Farzaneh¹,

Farzaneh²,

Mozdziak³

2017

JDVAR

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The chick embryo is a favorite and great in vivo model, which has long been used in embryology, developmental biology and applied sciences. Also, chicken embryo is the accessible model for transplantation and tracking studies. Avian multipotent stem cells have the capability to self-renew and differentiate into a variety of cell types in vitro. Avian multipotent stem cells were successfully isolated from bone marrow (BM), metanephric tissue, lung, amniotic cells, cartilage, and other embryonic and adult tissues. Subsequent analysis was performed to evaluate these multipotent cells in vitro. Results of the present study strongly contribute to the information related to the ability of avian multipotent stem cells to differentiate into cells such as adipocytes, osteoblasts, chondrocytes, astrocytes, and other committed cells. Avian multipotent stem cells may provide a new source of avian-derived cell lines for basic research and potential therapeutic applications.

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The influence of tissue microenvironment on stem cell–based cartilage repair

Cited by 46 publications

References 110 publications

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair

Concise Review: Avian Multipotent Stem Cells as a Novel Tool for Investigating Cell-Based Therapies

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