The Influence of the Testis on the Survival of Spermatozoa in the Epididymis of the Golden Hamster, Mesocricetus Auratus

Lubicz-Nawrocki, C. M.; Glover, T. D.

doi:10.1530/jrf.0.0340315

Cited by 37 publications

(30 citation statements)

References 12 publications

(12 reference statements)

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“…In the hamster, after castration sperm remained fertile for only half as long as following hemicastration. However, testosterone administration to castrated males resulted in fertilizing rates similar to those of hemicastrated males [5]. The present data on the fertilizing capacity of sperm following castration are in sharp contrast to those reported for other mammals and suggest that in the guinea pig testicular androgen may not be required to maintain fertilizing ability.…”

Section: Discussioncontrasting

confidence: 88%

“…Recent reports on sperm following castration or hypophysectomy of the rat [4], rabbit [2], and hamster [5] suggest a sharp and rapid decline in fertilizing ability. In the hamster, after castration sperm remained fertile for only half as long as following hemicastration.…”

Section: Discussionmentioning

confidence: 99%

“…The striking difference in the duration of fertilizing capacity of sperm from castrated versus testis(es)-intact males in such species as the hamster [5], rat [4], and rabbit [2], which was not observed in the guinea pig, may be related to interspecific variation.…”

Section: Discussionmentioning

confidence: 99%

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Morphology and Fertility of Guinea Pig Spermatozoa Aged In Vivo

Shepherd

Martan

1979

Archives of Andrology

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The morphology and fertility of spermatozoa from vasa deferentia of guinea pigs were observed following hemicastration or castration for approximately 40 days. The morphology of these aged sperm was studied from living and fixed preparations. Fertilizing ability was assayed by artificial insemination of estrous females and subsequent counting of embryos. Spermatozoa underwent morphological changes including dissociation of rouleaux, curving of tails, and loss of acrosomes; physio!ogical changes included a decline in the number of sperm with progressive motility and increased numbers of immotile spermatozoa with time after the operations. The fertilizing capacity of spermatozoa was maintained for approximately 30 days in both groups. However, sperm from one of the hemicastrated males resulted in conception 36 days postoperation. The data suggest that the loss of motility and decline in fertilizing ability were the result of spermatozoan senescence rather than testicular androgen deficiency.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Morphology and Fertility of Guinea Pig Spermatozoa Aged In Vivo

Shepherd

Martan

1979

Archives of Andrology

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“…Epididymides were dissected free of fat and connective tissue under sterile conditions in Hanks' balanced salt solution. Whole epididymides and epididymal segments [caput, zones 1-3 according to the Reid and Cleland classification (19); corpus, zone 4 and the proximal part of zone 5; and cauda, distal part of zone 5 25-cm2 tissue culture flasks at a cell density of 1 x 104 cells per ml and incubated in a humidified COJ air incubator at 320C.…”

Section: Methodsmentioning

confidence: 99%

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats.

Kierszenbaum¹,

Lea²,

Petrusz³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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A method for the isolation and culture of epididymal e ithelial cells obtained from pubertal and old adult rats is d red. This method permits the establishment of primary cultures of these cells in monolayers from aggregates isolated from whole epididymides and major epididymal anatomical segments (caput, corpus, and cauda) after trypsin and collagenase digestions. A large number of cultured epididymal cells retain a differentiated function as demonstrated by the immunocytochemical and radioimmunoassay finding of acidic epididymal glycoprotein, a spermatozoa-coating protein secreted by the principal cells of rat epididymis. The proliferative tential of cultured epididymal cells obtained from pubertal an old adult donors can be documented by [3H]thymidine labeling and mitotic indices without significant loss of gene expression for acidic epididymal glycoprotein.Results of this study demonstrate that epididymal epithelial cells, consisting of a predominant population of principal cells, can be isolated, cultured, and maintained for up to 3 months.

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“…Hence in most laboratory animals, castration or hypophysectomy results in a rapid decline in the development of sperm fertility within 4-7 days, and the loss of sperm viability after 1-2 weeks (Orgebin-Crist, Davies & Tichenor, 1973;Lubicz-Nawrocki & Glover, 1973). However, the nature of the androgen-dependent activities of the epididymis essential for sperm maturation and storage is not known.…”

Section: Introductionmentioning

confidence: 99%

Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster

Moore¹

1981

Reproduction

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Summary. Mature male rabbits and hamsters were bilaterally castrated and their epididymides were examined, at 2, 4, 7, 9 and 14 days after operation, for the presence of several glycoproteins by using monospecific antisera and indirect immunoperoxidase labelling. In the rabbit, there was a reduction in the glycoprotein reaction product in the epithelium of the proximal caput epididymidis by 2\p=n-\4days after castration, and the staining was weak or absent by 7\p=n-\9 days. Compared with controls, there was also a marked decrease in the intensity of reaction product to glycoprotein in the lumen of the proximal and distal corpus epididymidis 2\p=n-\4 days after castration although spermatozoa still filled the duct in this region. Glycoprotein was present in the distal cauda epididymidis at 14 days but staining diminished as spermatozoa were cleared from the tract. In the hamster, epididymal glycoproteins were apparently less critically dependent on androgens than those in the rabbit, reaching a weak but constant level in the proximal region by 7\p=n-\9 days after castration and remaining at the same intensity in the distal cauda epididymidis throughout the study.These results suggest that the secretion of specific glycoproteins by the mammalian epididymis is related to androgen levels and to sperm maturation.

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The Influence of the Testis on the Survival of Spermatozoa in the Epididymis of the Golden Hamster, Mesocricetus Auratus

Cited by 37 publications

References 12 publications

Morphology and Fertility of Guinea Pig Spermatozoa Aged In Vivo

Morphology and Fertility of Guinea Pig Spermatozoa Aged In Vivo

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats.

Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster

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