Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster

Moore, H. D. M.

doi:10.1530/jrf.0.0610347

Cited by 24 publications

(12 citation statements)

References 24 publications

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“…The best known are androgens, i.e., testosterone and its metabolite, dihydrotestosterone. Androgens regulate specific gene expression in the epididymal epithelium [7][8][9], and androgen withdrawal produces dramatic changes in the pattern of secreted proteins in every region [9]. Most of these changes are reversed by androgen administration [10,11].…”

Section: Introductionmentioning

confidence: 99%

Abdominal Temperature Induces Region-Specific p53-Independent Apoptosis in the Cauda Epididymidis of the Mouse1

Jara

Esponda²,

Carballada³

2002

Biology of Reproduction

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It is widely accepted that temperature regulates gene expression and function in the epididymis. However, the significance of reduced temperature of the scrotum in cell survival had not often been examined. Our hypothesis was that the experimental increase of the temperature could induce apoptosis. Using a surgical method that consists of surgically reflecting the cauda epididymidis in the abdomen, we have been able to show that this is the case. Apoptosis was examined by histologic procedures and by visualization of DNA fragmentation in agarose gels. We determined that the apoptosis is region-specific and affects only the principal cells of the proximal region of the cauda. It starts 12 h after surgery and ends by the third day. The apoptotic cells are eliminated by extrusion into the lumen and phagocytosis by adjacent cells. The complete molecular mechanism of apoptosis in this case remains unknown, but we have used the techniques of immunocytochemistry, Western blot, and reverse transcription-polymerase chain reaction to determine the role of some molecules. We have seen no significant role of androgens, the tumor suppressor p53, nor two heat shock proteins, hsp-25 and hsp-70. Nevertheless, we have detected a strong induction of bax and bcl-2 gene products. While the former should be responsible for the apoptosis observed, the latter would promote the survival of most of the cells of the cauda epididymis.

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Section: Introductionmentioning

confidence: 99%

Abdominal Temperature Induces Region-Specific p53-Independent Apoptosis in the Cauda Epididymidis of the Mouse1

Jara

Esponda²,

Carballada³

2002

Biology of Reproduction

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“…Castration has been shown to have adverse effects on nearly all epididymal functions and many of these depend on the presence of androgens (Orgebin-Crist et al, 1975;Robaire and Hermo, 1988). The synthesis and secretion of a large number of glycoproteins and the activity of a number of enzymes are to a large extent regulated by androgens (Bohmer et al, 1977;Jones et al, 1980;Brooks, 1981Brooks, ,1983Moore, 1981;Robaire and Hales, 1982). Testosterone replacement has been shown to prevent the negative effects on protein secretion and enzyme activity after castration or hypophy- sectomy (Dyson and Orgebin-Crist, 1973).…”

Section: Effects Of Castrationmentioning

confidence: 99%

Hormonal regulation of sulfated glycoprotein‐1 synthesis by nonciliated cells of the efferent ducts of adult rats

Rosenthal

Igdoura

Morales

et al. 1995

Molecular Reproduction Devel

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The objective of this study was to define the factors regulating the endogenous production of sulfated glycoprotein-1 (SGP-1) in nonciliated cells of the efferent ducts. To this end we examined five different groups of animals undergoing the following experimental procedures: (1) hypophysectomized animals at 7, 14, and 28 days, (2) 7-day hypophysectomized rats receiving testosterone implants given at various time intervals thereafter, (3) castration at various time intervals up to 7 days, (4) 7-day castrated rats receiving testosterone implants at various time intervals thereafter, and (5) castrated rats given testosterone implants immediately after castration and sacrificed at different time intervals thereafter. Efferent ducts were fixed by perfusion with 4% paraformaldehyde and 0.5% glutaraldehyde in phosphate buffer for quantitative immunocytochemical analysis at the level of the electron microscope. For each experimental condition and their controls, the number of gold particles/micron2 within the endosomal and lysosomal compartments was calculated taking into account the changes in both the volume of the cell and organelles being quantified and expressed as labeling content. The results revealed that hypophysectomy (up to 4 weeks) caused a marked significant decrease in the SGP-1 labeling content of the endosomal and lysosomal compartments. The labeling content of the lysosomal compartment of efferent ducts from rats castrated for up to 1 week did not change significantly. However, there was a significant decrease in the labeling content of endosomes. This decrease is due to SGP-1, which is secreted by Sertoli cells, not being available for uptake in the efferent ducts. These results suggested that testosterone is not required for maintaining the high labeling content of SGP-1 within lysosomes of nonciliated cells, but that a pituitary factor appears to be needed. The administration of testosterone at different intervals to 7-day castrated animals resulted in a significant decrease of lysosomal SGP-1, suggesting that testosterone under these experimental conditions inhibits the production of a pituitary factor that maintains the high labeling content of SGP-1 within lysosomes of the nonciliated cells. Testosterone administered to 7-day hypophysectomized animals over a 24-hr period had no effect on the labeling content of SGP-1 within lysosomes. However, the administration of testosterone to animals immediately following castration showed no differences in the labeling content of SGP-1 within compared to controls.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…This may reflect either a suppression of glycoprotein secretion or an increase in glycoprotein breakdown. Using monospecific antisera and immunoperoxidase labeling, however, Moore (1981) reported that specific glycoproteins are not secreted into the lumen of the rabbit epididymis after castration and that their synthesis is reduced. The glycoproteins can be visualized at the lu- menal border even when the immunostaining has disappeared from the epithelium and the lumen.…”

Section: Castrated Rabbitsmentioning

confidence: 99%

Secretion of proteins and glycoproteins by perifused rabbit corpus epididymal tubules: Effect of castration

et al. 1987

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Protein synthesis in epididymal tissue of intact and castrated rabbits was studied after incubation of epididymal minces with [35S]-cysteine or [35S]-methionine and protein separation by two-dimensional gel electrophoresis. Regional differences in the pattern of protein synthesized were observed. Castration did not change overall protein synthesis, but it reduced these regional differences. The presence of 5 alpha-DHT in the culture medium of the proximal corpus epididymidis perfused for 24 hr did not increase overall protein synthesis in tubules from intact or castrated rabbits and did not reinitiate synthesis of the proteins that had disappeared after castration. The kinetics of glycoprotein synthesis and secretion were studied by light and electron microscopy autoradiography at 0.5, 2, 6, and 24 hr after exposure to [3H]-mannose, [3H]-fucose, and [3H]-glucosamine. Changes in the distribution of mannose- and glucosamine-labeled material indicated that the decline in grain density over the epithelium from 30 min to 24 hr coincided with an increasing reaction over the stereocilia border from 30 min to 2 hr and in the lumen from 2 to 24 hr. The distribution of fucose-labeled material indicated that the grain reaction over the epithelium declined more rapidly than with the mannose label. When the glucosamine-labeled sperm mass was released from the tubules, the labeled material was lost after the first washing, indicating that the glucosamine-labeled glycoproteins did not bind firmly to corpus spermatozoa within 24 hr. After castration, both mannose- and fucose-labeled materials migrated to the cell apex more rapidly than in the intact animal, but they were not released as readily into the lumen. The culture of epididymal tubules from castrated males with 5 alpha-DHT for 24 hr did not promote the release of either mannose- or fucose-labeled material into the lumen. However, testosterone given in vivo for 2 weeks restored secretion of mannose-labeled material into the lumen.

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Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster

Cited by 24 publications

References 24 publications

Abdominal Temperature Induces Region-Specific p53-Independent Apoptosis in the Cauda Epididymidis of the Mouse1

Abdominal Temperature Induces Region-Specific p53-Independent Apoptosis in the Cauda Epididymidis of the Mouse1

Hormonal regulation of sulfated glycoprotein‐1 synthesis by nonciliated cells of the efferent ducts of adult rats

Secretion of proteins and glycoproteins by perifused rabbit corpus epididymal tubules: Effect of castration

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