The influence of the route of administration of gold nanoparticles on their tissue distribution and basic biochemical parameters: In vivo studies

Bednarski, Marek; Dudek, Magdalena; Knutelska, Joanna; Nowiński, Leszek; Sapa, Jacek; Zygmunt, Małgorzata; Nowak, Gabriel; Luty–Błocho, Magdalena; Fitzner, K.; Tesiorowski, Maciej

doi:10.1016/j.pharep.2014.10.019

Cited by 89 publications

(50 citation statements)

References 26 publications

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“…29,35 To examine the biocompatibility of PGNPs and SGNPs, their influence on blood biochemistry and liver and kidney function was studied in albino rabbits. All rabbits survived during the 3-day study period, and no change was observed in normal behavior, food intake, and Direct cT scan image of (A) PgNPs and (B) sgNPs shows that porous shape has a better density value and thus better contrast display.…”

Section: Results and Discussion Shape And Surface Morphology Of Npsmentioning

confidence: 99%

“…AST and ALP are studies. 29,35 However, this effect seems to be reversible as both the kidneys and liver revert to their normal function after clearance of nanoparticles ( Figure 6). Lack of toxicity has been broadly documented for negatively charged GNPs (probably due to their bare surface) in the size range reported in this paper and at a dose of 1 mg/kg.…”

Section: Results and Discussion Shape And Surface Morphology Of Npsmentioning

confidence: 99%

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Novel route synthesis of porous and solid gold nanoparticles for investigating their comparative performance as contrast agent in computed tomography scan and effect on liver and kidney function

Aziz¹,

Ihsan²,

Nazir³

et al. 2017

IJN

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Gold nanoparticles (GNPs) with dimension in the range of 1–100 nm have a prominent role in a number of biomedical applications like imaging, drug delivery, and cancer therapy owing to their unique optical features and biocompatibility. In this work, we report a novel technique for the synthesis of two types of GNPs namely porous gold nanoparticles (PGNPs) and solid gold nanoparticles (SGNPs). PGNPs of size 35 nm were fabricated by reduction of gold (III) solution with lecithin followed by addition of L-ascorbic acid and tri-sodium citrate, whereas SGNPs with a dimension of 28 nm were prepared by reflux method using lecithin as a single reducing agent. Comparative studies using PGNPs (λ max 560 nm) and SGNPs (λ max 548 nm) were conducted for evaluating their use as a contrast agent. These studies reveled that in direct computed tomography scan, PGNPs exhibited brighter contrast (45 HU) than SGNPs (26 HU). To investigate the effect of PGNPs and SGNPs on the liver and kidney profile, male rabbits were intravenously injected with an equal dose of 1 mg/kg weight of PGNPs and SGNPs. The effect on biochemical parameters was evaluated 72 hours after intravenous (IV) injection including liver function profile, renal (kidney) function biomarker, random blood glucose value, and cholesterol level. During one comparison of contrast in CT scan, PGNPs showed significantly enhanced contrast in whole-rabbit and organ CT scan as compared to SGNPs 6 hours after injection. Our findings suggested that the novel PGNPs enhance CT scan image with higher efficacy as compared to SGNPs. The results showed that IV administration of synthesized PGNPs increases the levels of aspartate aminotransferase (AST), alkaline phosphate (ALP), serum creatinine, and blood glucose, whereas that of SGNPs increases the levels of AST, ALP, and blood glucose.

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Section: Results and Discussion Shape And Surface Morphology Of Npsmentioning

confidence: 99%

Section: Results and Discussion Shape And Surface Morphology Of Npsmentioning

confidence: 99%

Novel route synthesis of porous and solid gold nanoparticles for investigating their comparative performance as contrast agent in computed tomography scan and effect on liver and kidney function

Aziz¹,

Ihsan²,

Nazir³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…[6] Indeed, some AuNPs formulations have already been proposed as novel tools for in vitro and in vivo molecular imaging and drug delivery. [7] AuNPs have, for example, attracted interest as carriers to enhance the oral absorption of drugs and vaccines that are either poorly absorbed or are susceptible to gastrointestinal degradation. [8] However, to our knowledge, only AuNPs used for local heat generation in the plasmonic photothermal therapy of atherosclerosis and cancer have, to date, reached clinical stage (ClinicalTrials.gov Identifiers NCT01270139, NCT01679470, NCT00848042, NCT00436410).…”

Section: Introductionmentioning

confidence: 99%

In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism

et al. 2017

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Gold nanoparticles (AuNPs) are present in many man-made products and cosmetics, and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and biological systems.Invertebrate models, such as the nematode Caenorhabditis elegans (C. elegans), can offer alternative approaches during the early phases of nanoparticle discovery.Here, we thoroughly evaluated the biodistribution of 11-nm and 150-nm citratecapped AuNPs in the model organism C. elegans at multiple scales, moving from micrometric to nanometric resolution and from the organismal to cellular level. We confirmed that the nanoparticles were not able to cross the intestinal and dermal barriers. We investigated the effect of AuNPs on the survival and reproductive performance of C. elegans, and correlated these effects with the uptake of AuNPs in terms of their number, surface area, and metal mass. In general, exposure to 11-nm AuNPs resulted in a higher toxicity than the larger 150-nm AuNPs. NP aggregation inside C. elegans was determined using absorbance microspectroscopy, which allowed the plasmonic properties of AuNPs to be correlated with their confinement inside the intestinal lumen, where anatomical traits, acidic pH and the presence of biomolecules play an essential role on NP aggregation. Finally, quantitative PCR of selected molecular markers indicated that exposure to AuNPs did not significantly affect 2 endocytosis and intestinal barrier integrity. Statement of significanceThis work highlights how the simple, yet information-rich, animal model C. elegans is ideally suited for preliminary screening of nanoparticles or chemicals mitigating most of the difficulties associated with mammalian animal models, namely the ethical issues, the high cost, and time constraints. This is of particular relevance to the cosmetic, food, and pharmaceutical industries, which all have to justify the use of animals, especially during the discovery, development and initial screening phases. This work provides a detailed and thorough analysis of 11-nm and 150-nm AuNPs at multiple levels of organization (the whole organism, organs, tissues, cells and molecules).

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“…During radiation, when cancerous cells are exposed to photons, a certain phenomenon, called photoelectric effect, occurs which absorbs a significant amount of the colliding radiation [187].…”

Section: Brain Targetsmentioning

confidence: 99%

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

Ajdari¹,

Moosavi²,

Rahmati³

et al. 2018

Preprint

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Nanoparticles (NPs) are widely used in diverse disciplines, including biology, medicine science. The central question that need to be answered is whether NPs have toxic effects on biological cells and molecules or are they safe. The safety of NPs including targeted drug delivery is critical and so is their toxicity in the environment. In recent years, in vitro and in vivo research on animals has generated abundant information about the toxicity of NPs.However, due to varying laboratory conditions, the comparison of the results from ensuing studies is somewhat unreliable. It should be noted that, depending on the type of production, NPs can enter the body through inhalation, skin and via digestive routes. Due to the diversity of NPs and their properties, there is paucity of accurate information on their toxicological effects; particle size, shape, surface area and the chemical levels are considered as key factors in creating health and toxicological effects. Consequently, there is a need for reliable information about their effects on various organs so as to deal with NPs effectively and their impact on health and the environment. This review covers the existing knowledge base on the subject that hopefully prepares us better to address these challenges.

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The influence of the route of administration of gold nanoparticles on their tissue distribution and basic biochemical parameters: In vivo studies

Cited by 89 publications

References 26 publications

Novel route synthesis of porous and solid gold nanoparticles for investigating their comparative performance as contrast agent in computed tomography scan and effect on liver and kidney function

Novel route synthesis of porous and solid gold nanoparticles for investigating their comparative performance as contrast agent in computed tomography scan and effect on liver and kidney function

In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism

Health concerns of various nanoparticles: A review of their in vitro and in vivo toxicity

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