The Influence of the Organic Matrix on Demineralization of Bovine Root Dentin in vitro

Abstract

The effect of matrix degradation on the rate of demineralization of dentin lesions was investigated. It was hypothesized that the demineralized matrix would inhibit the demineralization of the underlying mineralized dentin. Bovine root dentin specimens were alternately demineralized and incubated with either a bacterial collagenase or buffer (control). The demineralization was carried out under various conditions: Acetic acid solutions were used to form incipient and advanced erosive lesions, and lactic acid solutions containing a bisphosphonate were used to form incipient subsurface lesions. Under all conditions, the demineralization was found to be accelerated when the matrix was degraded by collagenase. This increase was more pronounced in advanced erosive lesions than in incipient lesions. Microscopic examination of collagenase-treated specimens revealed that the matrix of erosive lesions contained several layers of differently affected matrices, whereas the matrix of subsurface lesions appeared to be equally affected throughout the lesion. In conclusion, the matrix degradation was different in erosive and subsurface lesions but promoted the demineralization in both types of lesions.

Direct Bone Destruction by Neutrophils in Collagen-induced Arthritis Treated with Bisphosphonates

“…34,35) Furthermore, Kleter et al indicated that the decalcification of dentin was accelerated when the matrix was degraded by the alternate incubation of dentin with acid solution and collagenase. 36) All these results support the hypothesis that neutrophils can concurrently decalcify bone matrix mineral as well as degrade collagen fibers in the bone matrix.…”

The survival of organic matter in bone: a review

“…The presence of organic molecules will passivate the mineral surface (Putnis and Putnis 1995), slowing the rate of mineral solution reaction (Kleter et al 1994). Conversely, dissolution of the mineral phase will expose collagen to biodegradation (Krane 1975), thereby increasing the rate of its decomposition (Klont et al 1991).…”

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

“…In dentin, the presence of the demineralised organic hampers ionic diffusion and, thus, decreases the progression of erosive substance loss 1,2 . Previous in vitro studies showed that the enzymatic removal of the organic matrix by collagenase significantly increased the demineralisation process 2,3 . Considering that proteolytic enzymes like pepsin or trypsin are present in gastric juice, it was assumed that the erosion process in patients suffering from frequent vomiting and chronic reflux disease is at least partly affected by the degradation of the organic matrix 4,5 .…”