The Influence of the Dietary Cu-Glycine Complex on the Histomorphology of Cancellous Bone, Articular Cartilage, and Growth Plate as well as Bone Mechanical and Geometric Parameters Is Dose Dependent

Tomaszewska, Ewa; Dobrowolski, Piotr; Kwiecień, Małgorzata; Winiarska-Mieczan, Anna; Tomczyk, Agnieszka; Muszyński, Siemowit

doi:10.1007/s12011-016-0894-x

Cited by 31 publications

(20 citation statements)

References 32 publications

(43 reference statements)

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“…The thickness of the main zones: reserve zone (the zone I), proliferation zone (the zone II), hypertrophy zone (the zone III) and ossification zone (the zone IV) was measured at four sites along the growth plate cartilage as described previously [ 33 ]. Similarly, the thickness the following zones of the articular cartilage was measured: horizontal zone (superficial surface, the zone I), transitional zone (the zone II) and radial zone (the zone III) [ 34 ]. A total number of 200 measurements of each zone per group was performed (10 birds, 5 sections, 4 sites).…”

Section: Methodsmentioning

confidence: 99%

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Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens

Muszyński¹,

Tomaszewska²,

Kwiecień³

et al. 2018

PLoS ONE

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Zinc is required for normal bone development and cartilage formation. The purpose of this study was to assess the effect of with adding organic Zn (alone or phytase inclusion) at the reduced dose to growing male Ross 308 chickens on somatic axis and bone tissue metabolism. 200 one-day old broilers were divided into the negative control group fed diet without Zn or phytase inclusion, positive control group receiving Zn in the 100% of daily recommended dose from ZnO, and two experimental groups fed diet introduced Zn in 25% of daily recommendation as a glycine chelate (Zn-Gly) with or without phytase inclusion (500 FTUÁkg -1 ). Supplemental organic Zn increased bone Zn and Mg content, serum IGF-1, growth hormone and leptin concentration. Additional phytase inclusion increased body weight gain, blood plasma Ca, Fe, Zn and osteocalcin concentration and tibia ash percentage when compared to the Zn-deprived control. Bone geometry, yield and ultimate strengths were enhanced in both organic Zn supplemented groups, and the overall mechanical strength parameters of bone were better in these groups than in the positive control group supplemented with standard dose of inorganic Zn. Also marked improvements in the thickness of articular and the growth plate cartilages as well as real bone volume and thickness of metaphyseal trabeculae were achieved in all broilers fed Zn-supplemented diet irrespective of phytase inclusion, however, the highest cancellous bone mass and the best trabecular structure were noted after ZnO supplementation. In concludion, although dietary organic Zn given to growing broilers in 25% of daily recommended dose improved general bone properties and mechanical strength, the obtained results do not allow to unambiguously state that organic Zn supplementation at this level, even after phytase inclusion, is sufficient for proper bone development.

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Section: Methodsmentioning

confidence: 99%

“…After evaluating their mechanical properties, the tibiae were mineralized in a muffle furnace at 600°C to determine the percentage of bone ash [ 34 ]. The percentage of ash was determined relative to the wet bone weight.…”

Section: Methodsmentioning

confidence: 99%

Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens

Muszyński¹,

Tomaszewska²,

Kwiecień³

et al. 2018

PLoS ONE

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“…Pozytywny wpływ na ogólny wzrost kości i wzrost ilości tkanki kostnej w kości beleczkowej mogły mieć także obecne w badanym preparacie sole miedzi i cynku. Mikroelementy te są niezbędne w prawidłowym rozwoju, mineralizacji i utrzymaniu homeostazy tkanki kostnej, a ich obecność ma wpływ na wytrzymałość i sprężystość kości oraz aktywność osteoblastów (22,27,33).…”

Section: Ryc 3 Parametry Osteometryczne Kości Udowej Szczurówunclassified

Effect of an iodophor-silicate disinfecting and cleaning agent on liver function tests, the structure of the small intestine, and bone parameters in a rat model study

Muszyński¹,

Dobrowolski²,

Ivashkiv³

et al. 2019

Medycyna Weterynaryjna

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The aim of the study was to assess the risk of using an iodophor disinfecting and cleaning agent based on clinoptilolite intended for dry disinfection in the presence of non-ruminants. In a rat model study, a suspension containing 103.3 mg/kg b. w. of the disinfectant (a dose of 0.1 of LD50) was administrated per os for a period of 21 days. Its toxicity was assessed on the basis of liver function tests. The effects of the disinfectant on the histological parameters of the duodenum and jejunum and on the properties of long bones were determined. Although the agent showed no toxicity, at the end of the experiment the experimental animals had lower body weights than the control group animals (P < 0.01). The disinfectant exerted an adverse effect on the histological parameters of the jejunum, reducing the length of the intestinal villi (P < 0.01) and the absorption area (P < 0.05) and increasing the crypt depth (P < 0.05). On the other hand, the presence of clinoptilolite positively influenced the parameters of long bones, increasing their mass (P < 0.001), length (P < 0.001), density (P < 0.01), and ash content (P < 0.01). In the bone metaphysis and epiphysis root, a significant increase was found in the trabecular bone volume (P < 0.001) and the trabecular number (P < 0.05). Bone fracture strength was also increased (P < 0.05) in the experimental animals. The results obtained show that iodophores and clinoptilolite can be used in veterinary practice as components of low-toxic disinfecting and absorbing agents. However, the reduced body mass of the experimental animals suggests the need for further comprehensive tests aimed at determining the amount of the iodophor disinfecting and cleaning agent that does not impair performance parameters.

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“…Copper (Cu) as an essential trace element has many physiological functions in animals and humans, including skeletal mineralization, erythropoiesis, leukopoiesis, connective tissue synthesis, myelin formation, melanin pigment synthesis, catecholamine metabolism, thermal regulation, cholesterol metabolism, immune function, cardiac function, and glucose metabolic regulation [ 1 , 2 ]. It is known that Cu takes part in bone metabolism as an essential co-factor needed for the action of lysyl oxidase [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Inhibition of lysyl oxidase enzyme activity results in osteolathyrism and negatively affects bone and cartilage formation [ 5 ]. Furthermore, studies on Cu supplementation indicate that Cu deficiency leads to bone loss (osteopenia or osteoporosis), demineralization, a failure of ossification of growth centers, and neuropenia [ 1 , 2 ]. It is caused mainly by the decreased function of osteoblasts (bone tissue-forming cells) because the action of osteoclasts (bone tissue-resorbing cells) remains unaffected [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet

Muszyński

Tomaszewska

Kwiecień

et al. 2017

Biol Trace Elem Res

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Tibial mechanical, chemical, and histomorphometrical traits were investigated for growing male Ross 308 broiler chickens fed diets that had copper (Cu) from organic source at a lowered level of 25% of the daily requirement (4 mg kg−1 of a premix) with or without phytase. Dietary treatments were control non-copper, non-phytase group (0 Suppl); 4 mg kg−1 Cu non-phytase group (25%Cu); and 4 mg kg−1 Cu + 500 FTU kg−1 phytase group (25%Cu + phyt). The results show that birds fed with the addition of phytase exhibited improved weight gain and final body weight and had increased serum IGF-1 and osteocalcin concentrations. The serum concentration of Cu and P did not differ between groups; however, Ca concentration decreased in the 25%Cu + phyt group when compared to the 25%Cu group. Added Cu increased bone Ca, P, Cu, and ash content in Cu-supplemented groups, but bone weight and length increased only by the addition of phytase. Bone geometry, yield, and ultimate strengths were affected by Cu and phytase addition. A decrease of the elastic stress and ultimate stress of the tibia in Cu-supplemented groups was observed. The histomorphometric analysis showed a positive effect of Cu supplementation on real bone volume and trabecular thickness in the tibia metaphyseal trabeculae; additionally, phytase increased the trabeculea number. The supplementation with Cu significantly increased the total articular cartilage and growth plate cartilage thickness; however, the changes in thickness of particular zones were dependent upon phytase addition. In summary, dietary Cu supplements given to growing broilers with Cu in their diet restricted to 25% of the daily requirement had a positive effect on bone metabolism, and phytase supplementation additionally improved cartilage development.

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The Influence of the Dietary Cu-Glycine Complex on the Histomorphology of Cancellous Bone, Articular Cartilage, and Growth Plate as well as Bone Mechanical and Geometric Parameters Is Dose Dependent

Cited by 31 publications

References 32 publications

Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens

Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens

Effect of an iodophor-silicate disinfecting and cleaning agent on liver function tests, the structure of the small intestine, and bone parameters in a rat model study

Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet

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