The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (<i>Apis mellifera</i> L.)

Wilde, J.; Frączek, Regina; Siuda, M.; Bąk, Beata; Hatjina, Fani; Miszczak, A.

doi:10.1080/00218839.2016.1211394

Cited by 12 publications

(11 citation statements)

References 73 publications

(79 reference statements)

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“…Downregulation of most hemolymph proteases in workers exposed to IMD (Table I) is in line with the findings of Wilde et al (2016), although they analyzed homogenates from the entire worker body. So, we have shown that efficient proteolysis of the toxic peptides formed during antioxidative activity (Davies 1986) can be indirectly impaired by IMD.…”

Section: Proteolysis: Hypotheses 1 Andsupporting

confidence: 84%

Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

et al. 2020

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Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, and workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb and 200 ppb) in their diet. As a result, the colony depopulation did not occurred. IMD disturbed hemolymph/cuticle proteolysis; deactivated most of the cuticle protease inhibitors, activated hemolymph thiol and metal proteases and cuticle thiol proteases; downregulated ALP, ALT, AST; and increased DNA methylation in a caste-and age-dependent manner. The response in queens and workers differed, possibly due to eusocial evolution. Higher IMD dose had greater effects. The responses of ALP, ALT, AST, and DNA may reflect acceleration of biochemical senescence and epigenetic adaptation to IMD. All these biochemical side effects may lead to colony depopulation during future biotic/abiotic stress.

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Section: Proteolysis: Hypotheses 1 Andsupporting

confidence: 84%

Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

et al. 2020

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“…The reduction in protein content induced by emamectin benzoate + hexaflumuron in our study was similar to the finding of (Assar et al, 2016), who stated that total protein and lipid contents of S. littoralis larvae decreased with emamectin, hexaflumuron and teflubenzuron. (Wilde et al, 2016) reported that the level of protein in tissue extracts of A. mellifera significantly reduced from the 1 st to the 2 nd instars treated with imidacloprid. According to (Manal and Abdel-Mageed, 2018), the total protein content of M. domestica adults treated with tested mixtures such as deltamethrin+abamectin, lambada-cyhalothrin+khaya extract, indoxacarb+pomegranate, chlorantraniliprole+jojoba oil and methomyl+jojoba oil reduced compared with control.…”

Section: Discussionmentioning

confidence: 99%

Effects of emamectin benzoate combined with acetamiprid, eforia and hexaflumuron against Tuta absoluta (Lep.: Gelechiidae)

Talleh

Rafiee-Dastjerdi

Nasser

et al. 2020

Int. J. Adv. Biol. Biomed. Res.

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Tomato leafminer, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most destructive pests of tomato in many parts of the world including Iran. The toxicity of emamectin benzoate alone and combined with acetamiprid, eforia (thiamethoxam +lambda-cyhalotrin) and hexaflumuron were evaluated against 2 nd instar larvae of Tuta absoluta (Meyrick) under 25 ± 1 °C, the relative humidity of 75 ± 5% and a photoperiod of 16:8 (L:D). Also, the mixtures of tested insecticides with emamectin benzoate at LC15:LC15 ratio were evaluated on the activity of general esterase enzyme and total protein content of 2 nd instar larvae. After 96 hours, emamectin benzoate had the highest toxicity (LC50 = 0.52 mg A.I./L), followed by acetamiprid (LC50 = 56.39 mg A.I./L) and eforia (LC50 = 312.01 mg A.I./L). Hexaflumuron showed no toxicity against larvae. The combination of emamectin benzoate with acetamiprid at LC50:LC50 ratioproduced synergistic effects and all of the other ratios showed additive effects. The emamectin benzoate combined with either eforia or hexaflumuron at all of the ratios produced additive and antagonistic effects, respectively. Esterase activity of larvae increased when emamectin benzoate was mixed with either acetamiprid or eforia, but no significant differences were seen between emamectin benzoate alone and its mixture with hexaflumuron. The combination of emamectin benzoate with tested insecticides significantly reduced the total protein content of larvae. According to the results of this study, the mixtures of acetamiprid and eforia with emamectin benzoate showed higher negative impacts against 2 nd instar larvae than emamectin benzoate alone.

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“…preparaty grzybobójcze lub będące insektycydami, w tym środki ochrony roślin, pestycydy, a nawet środki stosowane do zwalczania np. komarów) (77,116,140). Ponadto pszczoły miodne są organizmami, których badanie dostarcza informacji o zmianach zachodzących w środowisku (49), zanieczyszczeniu (120), a także -co bardzo istotne ze względu na pogłębianie wiedzy epidemiologicznejo rozprzestrzenianiu się chorób owadów (szczególnie pszczół), rozwoju ich zjadliwości i mechanizmach epidemii (38,74).…”

Section: Analizy Leków Suplementów Diety I Preparatów Medycznych Lubunclassified

Honey bee as an alternative model invertebrate organism

Łoś¹,

Bieńkowska²,

Strachecka³

2025

Medycyna Weterynaryjna

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Insects perfectly fit the flagship principle of animal research – 3R: to reduce (the number of animals), to replace (animals with alternative models) and to refine (methods). Bees have the most important advantages of a model organism: they cause minimal ethical controversy, they have a small and fully known genome, and they permit the use of many experimental techniques. Bees have a fully functional DNMT toolkit. Therefore, they are used as models in biomedical/genetic research, e.g. in research on the development of cancer or in the diagnostics of mental and neuroleptic diseases in humans. The reversion of aging processes in bees offers hope for progress in gerontology research. The cellular mechanisms of learning and memory coding, as well as the indicators of biochemical immunity parameters, are similar or analogous to those in humans, so bees may become useful in monitoring changes in behavior and metabolism. Bees are very well suited for studies on the dose of the substance applied to determine the lethal dose or the effect of a formula on life expectancy. Honeybees have proven to be an effective tool for studying the effects of a long-term consumption of stimulants, as well as for observing behavioral changes and developing addictions at the individual and social levels, as well as for investigating the effects of continuously delivering the same dose of a substance. The genomic and physiological flexibility of bees in dividing tasks among workers in a colony makes it possible to create a Single- Cohort Colony (SCC) in which peers compared perform different tasks. Moreover behavioral methods (e.g. Proboscis Extension Reflex – PER, Sting Extension Reflex – SER, free flying target discrimination tasks or the cap pushing response) make it possible to analyse changes occurring in honeybee brains during learning and remembering. Algorithms of actions are created based on the behavior of a colony or individual, e.g. Artificial Bee Colony Algorithm (ABCA). Honeybees are also model organisms for profiling the so-called intelligence of a swarm or collective intelligence. Additionally, they serve as models for guidance systems and aviation technologies. Bees have inspired important projects in robotics, such as B-droid, Robobee and The Green Brain Project. It has also been confirmed that the apian sense of smell can be used to detect explosive devices, such as TNT, or drugs (including heroin, cocaine, amphetamines and cannabis). This inconspicuous little insect can revolutionize the world of science and contribute to the solution of many scientific problems as a versatile model.

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The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Cited by 12 publications

References 73 publications

Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Effects of emamectin benzoate combined with acetamiprid, eforia and hexaflumuron against Tuta absoluta (Lep.: Gelechiidae)

Honey bee as an alternative model invertebrate organism

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