Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Paleolog, Jerzy; Wilde, J.; Siuda, M.; Bąk, Beata; Wójcik, L; Strachecka, Aneta

doi:10.1007/s13592-020-00747-4

Cited by 24 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Imidacloprid (ID) [1-(6-chloro-3-pyridylmethyl)-2nitroimino-imidazolidine], which honey bees are exposed to by agriculture, has been found to be harmful to honey bees’ field navigation and health [ 11 , 12 , 13 ], including their detoxification abilities and reproductive functions [ 14 , 15 , 16 , 17 ]. Moreover, ID impairs the honey bee ADS and suppresses antioxidant genes [ 3 ], simultaneously increasing MDA (a marker of oxidative stress; lipid peroxidation) and shortening workers’ life-spans [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidation Defenses of Apis mellifera Queens and Workers Respond to Imidacloprid in Different Age-Dependent Ways: Old Queens Are Resistant, Foragers Are Not

Paleolog

Wilde

Miszczak

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

We investigated how different antioxidant defenses (ADSs) were shaped by evolution in young/old Apis mellifera workers and queens to broaden the limited knowledge on whether ADSs are effective in contemporary pesticide environments and to complete bee oxidative-aging theory. We acquired 1-day-old, 20-day-old, and 2-year-old queens and 1-day-old and 20-day-old workers (foragers) fed 0, 5, or 200 ppb imidacloprid, a pesticide oxidative stressor. The activities of catalase, glutathione peroxidase, glutathione S-transferase, and superoxide dismutase and the level of total antioxidant potential were determined in hemolymph. The ADS was upregulated in workers with age but downregulated in queens. Imidacloprid suppressed the ADS in all workers, particularly in foragers with an upregulated ADS, but it did not affect the ADS in 1-day-old queens. In contrast to foragers, the downregulated ADS of 2-year-old queens was unexpectedly highly upregulated by imidacloprid, which has not been previously shown in such old queens. The principal component analysis confirmed that queen and worker ADSs responded to imidacloprid in opposite ways, and ADS of 2-year-queens was markedly different from those of others. Thus, evolutionary shaped ADSs of older queens and workers may be of the limited use for foragers dwelling in pesticide ecosystems, but not for old queens.

show abstract

Section: Introductionmentioning

confidence: 99%

Antioxidation Defenses of Apis mellifera Queens and Workers Respond to Imidacloprid in Different Age-Dependent Ways: Old Queens Are Resistant, Foragers Are Not

Paleolog

Wilde

Miszczak

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…A similar study in bumble bees also showed no increase in viral titers when hives were placed near clothianidin-treated fields [151]. In addition, some studies have shown that hives supplemented with imidacloprid in their diet did not experience significant population losses compared to control hives [176], and others found no correlation between colony health and viral prevalence at agricultural sites [150].…”

Section: Laboratory-vs Field-based Studiesmentioning

confidence: 90%

Pesticide–Virus Interactions in Honey Bees: Challenges and Opportunities for Understanding Drivers of Bee Declines

Harwood¹,

Doležal²

2020

Viruses

View full text Add to dashboard Cite

Honey bees are key agricultural pollinators, but beekeepers continually suffer high annual colony losses owing to a number of environmental stressors, including inadequate nutrition, pressures from parasites and pathogens, and exposure to a wide variety of pesticides. In this review, we examine how two such stressors, pesticides and viruses, may interact in additive or synergistic ways to affect honey bee health. Despite what appears to be a straightforward comparison, there is a dearth of studies examining this issue likely owing to the complexity of such interactions. Such complexities include the wide array of pesticide chemical classes with different modes of actions, the coupling of many bee viruses with ectoparasitic Varroa mites, and the intricate social structure of honey bee colonies. Together, these issues pose a challenge to researchers examining the effects pesticide-virus interactions at both the individual and colony level.

show abstract

“…Additionally, after treating bees with imidacloprid, a decrease in acidic and alkaline protease activity and an increase in neutral protease activity was noticed regardless of the doses of insecticide (5 or 200 ppb) [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Honey Bee Proteolytic System and Behavior Parameters under the Influence of an Electric Field at 50 Hz and Variable Intensities for a Long Exposure Time

Migdał

Murawska

Strachecka

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

The effect of an artificial electromagnetic field on organisms is a subject of extensive public debate and growing numbers of studies. Our study aimed to show the effect of an electromagnetic field at 50 Hz and variable intensities on honey bee proteolytic systems and behavior parameters after 12 h of exposure. Newly emerged worker bees were put into cages and exposed to a 50 Hz E-field with an intensity of 5.0 kV/m, 11.5 kV/m, 23.0 kV/m, or 34.5 kV/m. After 12 h of exposure, hemolymph samples were taken for protease analysis, and the bees were recorded for behavioral analysis. Six behaviors were chosen for observation: walking, flying, self-grooming, contact between individuals, stillness, and wing movement. Bees in the control group demonstrated the highest number of all behavior occurrences, except flying, and had the lowest protease activity. Bees in the experimental groups showed a lower number of occurrences of walking, self-grooming, and contacts between individuals than the control bees and had significantly higher protease activity than the control bees (except that of alkaline proteases in the 23.0 kV/m group).

show abstract

Imidacloprid markedly affects hemolymph proteolysis, biomarkers, DNA global methylation, and the cuticle proteolytic layer in western honeybees

Cited by 24 publications

References 39 publications

Antioxidation Defenses of Apis mellifera Queens and Workers Respond to Imidacloprid in Different Age-Dependent Ways: Old Queens Are Resistant, Foragers Are Not

Antioxidation Defenses of Apis mellifera Queens and Workers Respond to Imidacloprid in Different Age-Dependent Ways: Old Queens Are Resistant, Foragers Are Not

Pesticide–Virus Interactions in Honey Bees: Challenges and Opportunities for Understanding Drivers of Bee Declines

Honey Bee Proteolytic System and Behavior Parameters under the Influence of an Electric Field at 50 Hz and Variable Intensities for a Long Exposure Time

Contact Info

Product

Resources

About