The influence of sperm-oocyte incubation time and breed of bull on in vitro embryo development in cattle

Sumantri, Cece; Ooe, Masato; Saha, Sourav Mohan; Boediono, Arief

doi:10.1016/0093-691x(96)84737-6

Cited by 9 publications

(8 citation statements)

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“…A relationship between the bull breed and the kinetics of acrosome changes in bovine spermatozoa was described by Sumantri et al (1996) and Demyda-Peyras et al (2012). In addition to that, our study confirms that individual bulls within a breed exhibit characteristic kinetics of acrosome changes.…”

Section: Discussionsupporting

confidence: 72%

“…In order to induce the acrosome reaction, culture media for in vitro fertilisation are supplemented with different capacitating agents, preferentially with heparin, which has a capacitating effect on bovine spermatozoa (Van Soom and de Kruif 1996;Pereira et al 2000;Mendes et al 2003). Sumantri et al (1996) demonstrated that under uniform capacitation conditions, the proportion of sperm capacitated at the same time is highly variable depending on the bull breed.…”

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confidence: 99%

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Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Rečková¹,

Machatkova²,

Máchal³

et al. 2015

Vet. Med.

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This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05). In conclusion, this study shows that the spermatozoa of bulls with a greater or intermediate response to heparin appear to be most suitable for in vitro embryo production compared with spermatozoa of bulls with no response to heparin.

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Section: Discussionsupporting

confidence: 72%

mentioning

confidence: 99%

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Rečková¹,

Machatkova²,

Máchal³

et al. 2015

Vet. Med.

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“…Shorter co‐incubation times can lead to higher oocyte fertilization (Gianaroli et al. 1996a,b) and blastocysts rates (Sumantri et al. 1996; Kochhar and King 1998; Grupen and Nottle 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed a negative effect of prolonged coincubation times during fertilization, probably because of production of reactive oxygen species by the spermatozoa (Gianaroli et al 1996b;Dirnfeld et al 1999) leading to detrimental embryonic development (Baker and Aitken 2004). Shorter co-incubation times can lead to higher oocyte fertilization (Gianaroli et al 1996a,b) and blastocysts rates (Sumantri et al 1996;Kochhar and King 1998;Grupen and Nottle 2000).…”

Section: Effects Of Co-incubation Time On Embryonic Developmentmentioning

confidence: 99%

In Vitro Fertilization using Non‐sexed and Sexed Bovine Sperm: Sperm Concentration, Sorter Pressure, and Bull Effects

Barceló-Fimbres¹,

Campos-Chillòn²,

Seidel

2010

Reprod Domestic Animals

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The objective of these experiments was to study bovine in vitro fertilization (IVF) conditions for blastocyst production using non-sexed sperm (Experiment 1) and sexed sperm (Experiment 2). For Experiment 1, in vitro-matured oocytes (N=707) were allocated to a 2 × 3 × 4 factorial design: time of co-incubation of gametes for fertilization (4 and 18 h), sperm dose (1, 0.33, and 0.11 × 10(6) frozen-thawed sperm/ml, and sperm source (four bulls). Pronuclear status was evaluated for a subset. Experiment 2 (N=2155 oocytes) was a 2 × 3 × 2 × 6 factorial design: sex of sperm (X and Y), sperm dose (1, 0.33, and 0.11 × 10(6) frozen-thawed sperm/ml), and sperm-sorting pressures (40 and 50 psi), replicated with sperm of six bulls. Presumptive zygotes were cultured 60 h in chemically defined medium-1 (CDM-1), and for 114 h in CDM-2. For Experiment 1, pronuclear formation, cleavage and blastocysts rates were greater for 1, and 0.33 × 10(6) than 0.11 × 10(6) sperm/ml (72 and 62 vs 42%; 89 and 81 vs 58%; and 21 and 17 vs 9%, respectively; all p<0.01); polyspermy was greater for 1, than 0.33 and 0.11 × 10(6) sperm concentrations (24 vs 2 and 0%; p<0.01). There were greater main effects (p<0.01) of pronuclear formation (69 vs 48%), polyspermy (13 vs 4%), and cleavage (63 vs 54%), at 18 than at 4 h of co-incubation of gametes (all p<0.01). For Experiment 2, cleavage and blastocyst rates were greater for 1 × 10(6) sperm/ml vs 0.33 and 0.11 (69%, 47%, and 30% cleavage and 30%, 14%, and 8% blastocysts) and 40 vs 50 psi (54% and 44% cleavage and 18% and 15% blastocysts) (p<0.01). A marked bull by fertilization sperm dose interaction was found for cleavage (p<0.05). The main conclusion was that the optimal sperm concentration for cleavage and producing blastocysts via IVF with sexed sperm was considerably higher and more variable among bulls than for unsexed sperm.

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“…It is clear, taking into account the available evidence, that male variability in IVF is a general feature of farm animal. Male variation in fertilization rates may possibly be due to differences in breed (Sumantri et al, 1996), season, age of animal, ejaculate sperm quality and other factors. Also it may be attributed to differences in metabolic characteristics of sperm cells (Brackett and Oliphant, 1975), seminal plasma content, the ratios of seminal plasma volume to sperm number (Fukui et al, 1988) and even within lots of semen from the same bull (Otoi et al, 1993).…”

Section: Discussionmentioning

confidence: 98%

Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays

Dayem¹,

Mahmoud²,

Nawito³

et al. 2009

Livestock Science

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The influence of sperm-oocyte incubation time and breed of bull on in vitro embryo development in cattle

Cited by 9 publications

References 0 publications

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

In Vitro Fertilization using Non‐sexed and Sexed Bovine Sperm: Sperm Concentration, Sorter Pressure, and Bull Effects

Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays

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