Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Abstract

This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05). In conclusion, this study shows that the spermatozoa of bulls with a greater or intermediate response to heparin appear to be most suitable for in vitro embryo production compared with spermatozoa of bulls with no response to heparin.

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

“…The findings in this study were in accordance with the report of [15] on Red Sokoto bucks, whose epididymal sperm livability declined in a storage-time dependent manner. The success of assisted reproductive techniques such as artificial insemination (AI) and in vitro fertilization (IVF) depends on the use of a sufficient number of motile acrosome intact sperm that has the potential of fertilizing the oocyte [35]. An intact acrosome prevents the loss of acrosomal enzymes such as hyaluronidase until they are released during acrosome reaction in the female reproductive tract prior to fertilization [36].…”

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review

“…During the fertilisation process, biophysical, biochemical, molecular and metabolic changes occur. https://doi.org/10.17221/48/2022-VETMED Some capacitating agents are secreted in the female reproductive tract during in vivo fertilisation (Maitan et al 2022), but need to add these agents in the in vitro fertilisation (IVF) medium during in vitro conditions (Reckova et al 2015). These agents, heparin, caffeine, calcium ionophore and PHE (d-penicillin, hypotaurine, and epinephrine) have been used singly or combined with others (Waheed et al 2016;El-Ruby et al 2017).…”

“…Some capacitating agents are secreted in the female reproductive tract during in vivo fertilisation ( Maitan et al. 2022 ), but need to add these agents in the in vitro fertilisation (IVF) medium during in vitro conditions ( Reckova et al. 2015 ).…”