The Influence of Sex and Ovarian Hormones on Temporomandibular Joint Nociception in Rats

Fischer, Luana; Torres-Chávez, Karla Elena; Clemente-Napimoga, Juliana Trindade; Jorge, Dany; Arsati, Franco; Veiga, Maria Cecı́lia Ferraz de Arruda; Tambeli, Cláudia Herrera

doi:10.1016/j.jpain.2008.02.006

Cited by 76 publications

(98 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, formalin injection also induces plasma extravasation [8,9], leukocyte migration [10], and cytokines release [11]. We have adapted the formalin test to the TMJ [12] and we have recently demonstrated that formalin-induced nociceptive behavior is higher during low ovarian hormone levels of the estrous cycle in rats [13,14]. This finding is in accordance with a clinical study showing that TMJ pain in women is higher during low hormonal level of the menstrual cycle [15].…”

Section: Introductionsupporting

confidence: 77%

“…Proestrus phase and the initial phase of diestrus were chosen because they represent phases of high and low serum estradiol level, respectively [14].…”

Section: Estrous Phase Determinationmentioning

confidence: 99%

“…Gonadectomy was performed in 45-day-old animals, as previously described [14]. Ovariectomy was performed through bilateral upper flank incisions.…”

Section: Gonadectomymentioning

confidence: 99%

See 2 more Smart Citations

Sexual Dimorphism on Cytokines Expression in the Temporomandibular Joint: The Role of Gonadal Steroid Hormones

et al. 2010

Self Cite

View full text Add to dashboard Cite

Temporomandibular joint pain-related conditions are generally characterized by local inflammation; however, little studies have focused on the role of gonadal hormones in the expression of inflammatory mediators, such as cytokines. Therefore, we asked whether gonadal steroid hormones affect formalin-induced cytokines expression in the rat temporomcandibular joint. The expression of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and cytokine-induced neutrophil chemoattractant (CINC)-1 was significantly higher in males than in diestrus and proestrus females and was decreased by orchiectomy and restored by testosterone replacement. The expression of IL-6 was significantly higher in diestrus and proestrus females than in males, and was decreased by ovariectomy and restored by estradiol or progesterone administration. We conclude that testosterone increases the expression of TNF-α, IL-1β and CINC-1, and estradiol and progesterone increase the expression of IL-6. New clinical approaches based on inhibition of pro-inflammatory mediators are starting to supplant traditional immunosuppressive therapies and gonadal hormones may influence their effectiveness or clinical dosage.

show abstract

Section: Introductionsupporting

confidence: 77%

“…Proestrus phase and the initial phase of diestrus were chosen because they represent phases of high and low serum estradiol level, respectively [14].…”

Section: Estrous Phase Determinationmentioning

confidence: 99%

See 1 more Smart Citation

Sexual Dimorphism on Cytokines Expression in the Temporomandibular Joint: The Role of Gonadal Steroid Hormones

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…The latencies to tailflick and pawlick in response to radiant heat were increased in pro-estrous rats, with higher concentrations of estradiol and progesterone [4][5][6]. The temporomandibular joint nociceptive behavior of females with proestrus levels of estradiol was significantly lower than that in the diestrus phase [7]. Ovariectomized (OVX) mice developed a robust mechanical hyperalgesia and allodynia [8], which could be reversed by estrogen [9].…”

mentioning

confidence: 99%

Estrogen modulation of peripheral pain signal transduction: involvement of P2X3 receptors

Jin

et al. 2011

Purinergic Signalling

View full text Add to dashboard Cite

There is evidence that gonadal hormones may affect the perception of painful stimulation, although the underlying mechanisms remain unclear. This investigation was undertaken to determine whether the adenosine 5′-triphosphate (ATP) receptor subunit, P2X 3 , is involved in the modulatory action of estrogen in peripheral pain signal transduction in dorsal root ganglion (DRG). The mechanical pain behavior test, real-time quantitative reverse transcription-polymerase chain reaction analysis, and Western blot methods were used to determine the mean relative concentrations and functions of P2X 3 receptors in DRG in sham, ovariectomized (OVX), and estradiol replacement (OVX+E 2 ) female rats and in sham and orchiectomized male rats. The mechanical hyperalgesia appeared after ovariectomy, which was subsequently reversed after estradiol replacement, whereas it was not observed after orchiectomy in male rats. Plantar injection of 2′(3′)-O-(2,4,6-trinitrophenyl) ATP (TNP-ATP), a P2X 3 and P2X 2/3 receptor antagonist, resulted in an increase of the pain threshold force in OVX rats while had no effect on sham rats. Furthermore, A-317491, a selective P2X 3 /P2X 2/3 receptor antagonist, significantly reversed the hyperalgesia of OVX rats. Injection of ATP into the plantars also caused a significant increase of the paw withdrawal duration in OVX rats compared with that seen in the sham group, which became substantially attenuated by TNP-ATP. P2X 3 receptors expressed in DRG were significantly increased in both mRNA and protein levels after ovariectomy and then reversed after estrogen replacement, while a similar increase was not observed after orchiectomy in male rats. Furthermore, P2X 3 mRNA was significantly decreased 24 h after the application of 17β-estradiol in a concentrationdependent manner in cultured DRG neurons. ICI 182,780, an estrogen receptor antagonist, blocked the reduction in the protein level. These results suggest that the female gonadal hormone, 17β-estradiol, might participate in the control of peripheral pain signal transduction by modulating P2X 3 receptor-mediated events in primary sensory neurons, probably through genomic mechanisms.

show abstract

“…21,28,30,31,64 As with mustard oil, the main literature on the use of formalin is dominated by one group, in this case by Tambeli and coworkers. Unfortunately, not many details were given on how the TMJ was identified to compare it to other irritants.…”

Section: Formalinmentioning

confidence: 99%

Animal Models of Temporomandibular Joint Disorders: Implications for Tissue Engineering Approaches

2011

View full text Add to dashboard Cite

Animal models for temporomandibular joint disorder (TMD) or degradation are necessary for assessing the value of current and future tissue engineering therapies. After reviewing the literature, it is quite apparent that most TMD animal studies can be categorized into chemical approaches or surgical/mechanical approaches. Overall, it was found that the top five cited manuscripts for all chemical models were cited by almost 40% more manuscripts than the top five manuscripts for surgical/mechanical models. It is clear that the chemical approaches have focused on the inflammatory aspect of TMDs and its relationship to pain. However, chemical irritants must be tested in larger animal models, and the effect of short-term inflammation on the mechanical properties of the fibrocartilage must be examined. Nevertheless, therapeutic approaches aimed at reducing or controlling inflammation could use the established chemical methods. Surgical/mechanical methods can be used as negative controls for first generation TMJ tissue engineering approaches when the therapy is applied immediately after injury. Next generation tissue engineering approaches will require testing on tissues degenerated for a few months after the surgical/mechanical methods, with enhanced functional assessment techniques.

show abstract

The Influence of Sex and Ovarian Hormones on Temporomandibular Joint Nociception in Rats

Cited by 76 publications

References 45 publications

Sexual Dimorphism on Cytokines Expression in the Temporomandibular Joint: The Role of Gonadal Steroid Hormones

Sexual Dimorphism on Cytokines Expression in the Temporomandibular Joint: The Role of Gonadal Steroid Hormones

Estrogen modulation of peripheral pain signal transduction: involvement of P2X3 receptors

Animal Models of Temporomandibular Joint Disorders: Implications for Tissue Engineering Approaches

Contact Info

Product

Resources

About