The influence of quaternary structure on the stability of Fenna–Matthews–Olson (FMO) antenna complexes

Saer, Rafael G.; Schultz, Rebecca L.; Blankenship, Robert E.

doi:10.1007/s11120-018-0591-z

Cited by 3 publications

(4 citation statements)

References 37 publications

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“…A number of single-point and double FMO mutants targeting the electronic properties of individual pigments and the structural integrity of the complex have been synthesized. ,− For example, Saer et al engineered and characterized eight site-directed mutants in which changes were introduced into protein pockets housing each FMO pigment . It was shown that in all but one mutant the associated changes in absorption and circular dichroism (CD) spectra could be modeled by a spectral shift of the excitation energy of the specific pigment targeted by a mutation.…”

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confidence: 99%

Predicting Mutation-Induced Changes in the Electronic Properties of Photosynthetic Proteins from First Principles: The Fenna–Matthews–Olson Complex Example

Kim,

Mitchell,

Lawrence

et al. 2023

J. Phys. Chem. Lett.

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Multiscale molecular modeling is utilized to predict optical absorption and circular dichroism spectra of two single-point mutants of the Fenna−Matthews−Olson photosynthetic pigment−protein complex. The modeling approach combines classical molecular dynamics simulations with structural refinement of photosynthetic pigments and calculations of their excited states in a polarizable protein environment. The only experimental input to the modeling protocol is the X-ray structure of the wild-type protein.The first-principles modeling reproduces changes in the experimental optical spectra of the considered mutants, Y16F and Q198V. Interestingly, the Q198V mutation has a negligible effect on the electronic properties of the targeted bacteriochlorophyll a pigment. Instead, the electronic properties of several other pigments respond to this mutation. The molecular modeling demonstrates that a single-point mutation can induce long-range effects on the protein structure, while extensive structural changes near a pigment do not necessarily lead to significant changes in the electronic properties of that pigment.

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confidence: 99%

Predicting Mutation-Induced Changes in the Electronic Properties of Photosynthetic Proteins from First Principles: The Fenna–Matthews–Olson Complex Example

Kim,

Mitchell,

Lawrence

et al. 2023

J. Phys. Chem. Lett.

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“…Equation ( 17) combined with Equations ( 18) and ( 19) is used for fitting the correlation function and spectral density can be computed by substituting γ, γ and λ into Equation (16).…”

Section: Calculation Of Energies and Spectral Density Of Fmo Complexmentioning

confidence: 99%

“…In photosynthetic green sulfur bacteria, the specialized light-absorbing green pigments called chlorophyll absorbs the light photons that are further transferred through a water-soluble pigment-protein complex known as FMO to the reaction center to drive the charge separation [3]. Owing to the important role played by the FMO complex in the energy transfer in green sulfur bacteria, significant research efforts have been made in the past decade for our better understanding of their optical properties utilizing a wide range of spectroscopic and theoretical approaches, making it an ideal protein model system for studying exciton dynamics and excitation energy transfer in the photosynthetic complexes [3,4,[11][12][13][14][15][16]. In particular, we recall that the FMO protein complex contains seven BChl a molecules wrapped in a string bag of protein that plays an important role in green sulfur bacteria, connecting the chlorosome antenna to the reaction center where the photosynthesis events occur.…”

Section: Introductionmentioning

confidence: 99%

“…Experimentally, the eighth BChl pigment has also been newly resolved in the FMO protein that lies perpendicular to the plane of the other seven BChls [17,18]. Notably, FMO complex is a homotrimer of three ≈ 40 kDa monomer that possesses C3 symmetry whereby three copies of each subunit of seven BChl a chromophores bind together to the eighth BChl a pigment [16,19,20]. Due to close packing of BChl a pigments in each monomer subunit, the excited electronic states of FMO complexes are delocalized over multiple pigments.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Photosynthetic Systems and Their Applications with Mathematical and Computational Models

2020

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In biological and life science applications, photosynthesis is an important process that involves the absorption and transformation of sunlight into chemical energy. During the photosynthesis process, the light photons are captured by the green chlorophyll pigments in their photosynthetic antennae and further funneled to the reaction center. One of the most important light harvesting complexes that are highly important in the study of photosynthesis is the membrane-attached Fenna–Matthews–Olson (FMO) complex found in the green sulfur bacteria. In this review, we discuss the mathematical formulations and computational modeling of some of the light harvesting complexes including FMO. The most recent research developments in the photosynthetic light harvesting complexes are thoroughly discussed. The theoretical background related to the spectral density, quantum coherence and density functional theory has been elaborated. Furthermore, details about the transfer and excitation of energy in different sites of the FMO complex along with other vital photosynthetic light harvesting complexes have also been provided. Finally, we conclude this review by providing the current and potential applications in environmental science, energy, health and medicine, where such mathematical and computational studies of the photosynthesis and the light harvesting complexes can be readily integrated.

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Engineering microbial metabolic energy homeostasis for improved bioproduction

Tong

Chen

et al. 2021

Biotechnology Advances

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The influence of quaternary structure on the stability of Fenna–Matthews–Olson (FMO) antenna complexes

Cited by 3 publications

References 37 publications

Predicting Mutation-Induced Changes in the Electronic Properties of Photosynthetic Proteins from First Principles: The Fenna–Matthews–Olson Complex Example

Predicting Mutation-Induced Changes in the Electronic Properties of Photosynthetic Proteins from First Principles: The Fenna–Matthews–Olson Complex Example

Analysis of Photosynthetic Systems and Their Applications with Mathematical and Computational Models

Engineering microbial metabolic energy homeostasis for improved bioproduction

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