Engineering microbial metabolic energy homeostasis for improved bioproduction

Tong, Tian; Chen, Xiulai; Hu, Guipeng; Wang, Xiaoling; Liu, Gao-Qiang; Li, Liming

doi:10.1016/j.biotechadv.2021.107841

Cited by 11 publications

(4 citation statements)

References 160 publications

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“…It is widely used to produce methyl methacrylate (MMA), an important basic organic chemical material, because it has two functional groups, carbon double bond and a carboxyl group, and can conduct polymerization and esterification reactions. In addition, MAA can also be used as raw materials for synthetic rubber, thermosetting paint, synthetic rubber, fabric treatment agent, etc. − With the continuous attention paid to biomass fuel, a large number of experiments have shown that the chemicals and intermediates produced by biomass fuel can effectively replace some coal-based materials, which not only compound the concept of environment friendly, but also can develop downstream industries for industrialization and commercialization. − One of the fields is to synthesize important vinyl polymers in the industry from biomass raw materials, such as methacrylic acid (MAA) and methyl methacrylate (MMA) …”

Section: Introductionmentioning

confidence: 99%

Liquid–Liquid Equilibrium for Ternary Systems of Water, Methacrylic Acid, and Alkyl Alcohols: Thermodynamic Analysis and Solvent Comparison

Han,

Zhen,

Zhang

et al. 2024

J. Chem. Eng. Data

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The development and research of biomass fuels have been paid more and more attention. The production of methacrylic acid is accompanied by a large amount of water and environmental pollution. Therefore, the separation of methacrylic acid from aqueous solution is of great significance for industrial production and environmental protection. The liquid−liquid equilibrium (LLE) for ternary systems of water + methacrylic acid + alcohol solvents at 303.2 K and 101.3 kPa was investigated. Thermodynamic models (NRTL and UNIQUAC) were used to correlate and regress the experimental data. The root-mean-square deviation (RMSD %) was used to evaluate the deviation between the experimental value and the calculated value, which were 1 and 1, respectively, indicating that the extractants selected in the experiment could well separate methacrylic acid from the aqueous solution, and 1-octanol exhibits better extraction performance. The correlation of LLE data is proven by MATLAB. In addition, the binary interactive parameters of the model regression are verified by MATLAB.

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Section: Introductionmentioning

confidence: 99%

Liquid–Liquid Equilibrium for Ternary Systems of Water, Methacrylic Acid, and Alkyl Alcohols: Thermodynamic Analysis and Solvent Comparison

Han,

Zhen,

Zhang

et al. 2024

J. Chem. Eng. Data

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“…Metabolic engineering provides promising strategies to improve the production of natural products, such as enhancing and optimizing the biosynthesis pathway [ 17 – 19 ], eliminating the competitive pathway [ 18 , 20 , 21 ], and engineering energy homeostasis [ 22 , 23 ]. Several strategies have been successfully applied to improve the production of echinocandins.…”

Section: Introductionmentioning

confidence: 99%

Improving the production of the micafungin precursor FR901379 in an industrial production strain

et al. 2023

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Background Micafungin is an echinocandin-type antifungal agent used for the clinical treatment of invasive fungal infections. It is semisynthesized from the sulfonated lipohexapeptide FR901379, a nonribosomal peptide produced by the filamentous fungus Coleophoma empetri. However, the low fermentation efficiency of FR901379 increases the cost of micafungin production and hinders its widespread clinical application. Results Here, a highly efficient FR901379-producing strain was constructed via systems metabolic engineering in C. empetri MEFC09. First, the biosynthesis pathway of FR901379 was optimized by overexpressing the rate-limiting enzymes cytochrome P450 McfF and McfH, which successfully eliminated the accumulation of unwanted byproducts and increased the production of FR901379. Then, the functions of putative self-resistance genes encoding β-1,3-glucan synthase were evaluated in vivo. The deletion of CEfks1 affected growth and resulted in more spherical cells. Additionally, the transcriptional activator McfJ for the regulation of FR901379 biosynthesis was identified and applied in metabolic engineering. Overexpressing mcfJ markedly increased the production of FR901379 from 0.3 g/L to 1.3 g/L. Finally, the engineered strain coexpressing mcfJ, mcfF, and mcfH was constructed for additive effects, and the FR901379 titer reached 4.0 g/L under fed-batch conditions in a 5 L bioreactor. Conclusions This study represents a significant improvement for the production of FR901379 and provides guidance for the establishment of efficient fungal cell factories for other echinocandins.

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“…As LA accumulation is NADH-dependent, this method regulates the LA fraction by increasing the NADH supply. One of the most effective ways to increase the NADH supply under aerobic conditions is to control the flux of the electron transport chain (ETC), also known as the respiratory chain, which converts reducing agents such as NADH into ATP. − Because the respiratory chain is vital, it cannot be overly downregulated; thus, this strategy employs a flexible and controllable “metabolic transistor” to control the sink strength of the respiratory chain in E. coli by controlling the expression level of the 4-hydroxybenzoate geranyltransferase gene from Lithospermum erythrorhizon ( lepgt , encoding le PGT-1). , This approach was successfully used to regulate the LA fraction in P(3HB- co -LA) by using glucose as a substrate, and the LA fraction could be further improved by changing glucose to xylose, a carbon source that is highly abundant in lignocellulose, as a substrate.…”

Section: Introductionmentioning

confidence: 99%

Fine-Tuning Biosynthesis of Biodegradable Poly(3-hydroxybutyrate-co-lactate) with Different Ratios of d-Lactate from Xylose by Engineered Escherichia coli

Wu,

Gong,

Sun

et al. 2023

ACS Sustainable Chem. Eng.

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The toughness and transparency of poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] depend on its lactate (LA) fraction. In Escherichia coli, LA production can be increased by restricting the activity of the electron transport chain (ETC). As ubiquinone-8 (Q8) is essential to mediate electron transfer, it is one target to modulate the ETC in E. coli. 4-Hydroxybenzoate geranyltransferase (lePGT-1, encoded by the lepgt gene) from Lithospermum erythrorhizon can competitively consume substrates required for the biosynthesis of Q8. Therefore, flexible regulation of LA production can be achieved by controlling the expression level of the lepgt gene to precisely control the flux of the ETC. This strategy was applied for the regulation of the molar percentage (mol %) of the LA fraction in P(3HB-co-LA). Using xylose as a carbon source instead of glucose increased the LA fraction to 10.6–27.7 mol %, and deleting cytochrome oxidase genes further increased the LA fraction to 29.3–41.8 mol %. Finally, the stability and effectiveness of the flexible control strategy were verified by a bioreactor study.

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Engineering microbial metabolic energy homeostasis for improved bioproduction

Cited by 11 publications

References 160 publications

Liquid–Liquid Equilibrium for Ternary Systems of Water, Methacrylic Acid, and Alkyl Alcohols: Thermodynamic Analysis and Solvent Comparison

Liquid–Liquid Equilibrium for Ternary Systems of Water, Methacrylic Acid, and Alkyl Alcohols: Thermodynamic Analysis and Solvent Comparison

Improving the production of the micafungin precursor FR901379 in an industrial production strain

Fine-Tuning Biosynthesis of Biodegradable Poly(3-hydroxybutyrate-co-lactate) with Different Ratios of d-Lactate from Xylose by Engineered Escherichia coli

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