The influence of processes for the purification of human bone allografts on the matrix surface and cytocompatibility

Dumas, Aline; Gaudin-Audrain, Christine; Mabilleau, Guillaume; Massin, Phillipe; Hubert, Laurent; Basle, Michel‐Félix; Chappard, Daniel

doi:10.1016/j.biomaterials.2006.03.044

Cited by 29 publications

(26 citation statements)

References 39 publications

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“…3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Assay-Cell proliferation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as described previously (23).…”

Section: Methodsmentioning

confidence: 99%

Thiazolidinediones Induce Osteocyte Apoptosis by a G Protein-coupled Receptor 40-dependent Mechanism

Mieczkowska

Basle

Chappard

et al. 2012

Journal of Biological Chemistry

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Background: Thiazolidinediones (TZDs) mediate osteocyte apoptosis and sclerostin up-regulation by an unknown mechanism. Results: Osteocyte apoptosis is mediated through activation of Erk1/2 and p38, whereas sclerostin up-regulation is through peroxisome proliferator-activated receptor-␥ (PPAR␥) signaling. Conclusion: TZDs signal not exclusively through PPAR␥, as thought, but also via a surface receptor called GPR40. Significance: Learning how TZDs signal in bone cells is crucial to prevent adverse effects associated with the use of these drugs.

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Section: Methodsmentioning

confidence: 99%

Thiazolidinediones Induce Osteocyte Apoptosis by a G Protein-coupled Receptor 40-dependent Mechanism

Mieczkowska

Basle

Chappard

et al. 2012

Journal of Biological Chemistry

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“…Standard chemical processing protocols (NaOH and hydrogen peroxide) eradicate osteoid staining in human trabecular bone samples and alter the structure and organization of collagen fibrils. 1 These surface changes led to decreased adhesion of osteoblasts and decreased AP activity relative to normal bone. 1 In addition, gamma-irradiation, which is often the terminal event in graft sterilization, can produce free radicals that target collagen and alter structural properties of bone.…”

Section: Discussionmentioning

confidence: 99%

“…1 These surface changes led to decreased adhesion of osteoblasts and decreased AP activity relative to normal bone. 1 In addition, gamma-irradiation, which is often the terminal event in graft sterilization, can produce free radicals that target collagen and alter structural properties of bone. 33 These studies indicate the importance of a resurfacing approach to improve allograft healing, and both HAP 9,10 and nanotopography 6 have been identified as potential approaches.…”

Section: Discussionmentioning

confidence: 99%

“…Bones were processed in 95% ethanol for 3 h, and 100% ethanol for 2 h, as previously described, 1 and frozen at -80°C for one week.…”

Section: Bone Processingmentioning

confidence: 99%

“…These processing procedures not only render the graft avascular and acellular, but also alter surface characteristics of bone, 1,2 and decrease the ability of osteoblasts to adhere and differentiate on the surface. 1 Because the surface of cadaveric bone is altered during chemical processing 1,2 and surfaces of many synthetic implants are not osteoconductive, 3,4 a good deal of research has been focused on surface modification of bone grafts and implants. [5][6][7][8][9][10] Biomaterial surface characteristics that can affect cell behavior include surface topography, chemistry, and energy.…”

Section: Introductionmentioning

confidence: 99%

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Specific Biomimetic Hydroxyapatite Nanotopographies Enhance Osteoblastic Differentiation and Bone Graft Osteointegration

Loiselle

Wei

Faryad

et al. 2013

Tissue Engineering Part A

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Impaired healing of cortical bone grafts represents a significant clinical problem. Cadaveric bone grafts undergo extensive chemical processing to decrease the risk of disease transmission; however, these processing techniques alter the bone surface and decrease the osteogenic potential of cells at the healing site. Extensive work has been done to optimize the surface of bone grafts, and hydroxyapatite (HAP) and nanotopography both increase osteoblastic differentiation. HAP is the main mineral component of bone and can enhance osteoblastic differentiation and bone implant healing in vivo, while nanotopography can enhance osteoblastic differentiation, adhesion, and proliferation. This is the first study to test the combined effects of HAP and nanotopographies on bone graft healing. With the goal of identifying the optimized surface features to improve bone graft healing, we tested the hypothesis that HAP-based nanotopographic resurfacing of bone grafts improves integration of cortical bone grafts by enhancing osteoblastic differentiation. Here we show that osteoblastic cells cultured on processed bones coated with specific-scale (50-60 nm) HAP nanotopographies display increased osteoblastic differentiation compared to cells on uncoated bone, bones coated with poly-l-lactic acid nanotopographies, or other HAP nanotopographies. Further, bone grafts coated with 50-60-nm HAP exhibited increased formation of new bone and improved healing, with mechanical properties equivalent to live autografts. These data indicate the potential for specific HAP nanotopographies to not only increase osteoblastic differentiation but also improve bone graft incorporation, which could significantly increase patient quality of life after traumatic bone injuries or resection of an osteosarcoma.

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Chitosan Based Composites and Their Applications in Tissue Engineering

Khan

Shah

Nawaz

et al. 2020

Encyclopedia of Marine Biotechnology

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The influence of processes for the purification of human bone allografts on the matrix surface and cytocompatibility

Cited by 29 publications

References 39 publications

Thiazolidinediones Induce Osteocyte Apoptosis by a G Protein-coupled Receptor 40-dependent Mechanism

Thiazolidinediones Induce Osteocyte Apoptosis by a G Protein-coupled Receptor 40-dependent Mechanism

Specific Biomimetic Hydroxyapatite Nanotopographies Enhance Osteoblastic Differentiation and Bone Graft Osteointegration

Chitosan Based Composites and Their Applications in Tissue Engineering

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