The influence of pre- and post-ovulatory insemination on sperm distribution in the oviduct, accessory sperm to the zona pellucida, fertilisation rate and embryo development in sows

Kaeoket, Kampon; Persson, Erik; Dalin, Anne-Marie

doi:10.1016/s0378-4320(02)00022-2

Cited by 27 publications

(51 citation statements)

References 29 publications

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“…The results of plasma hormone levels (Fig 1a and 1b) were shown previously by Kaeoket et al (2001Kaeoket et al ( , 2002 for cyclic and inseminated sows, respectively. The cyclic sows (experiment A) showed high plasma levels of oestradiol17b at prooestrus and oestrus and a high level of progesterone during dioestrus (Fig.…”

Section: Plasma Hormone Levels and Pregnancy Confirmationsupporting

confidence: 82%

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Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong¹,

Dalin²,

Sahlin³

et al. 2005

Reproduction

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Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5 -6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

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Section: Plasma Hormone Levels and Pregnancy Confirmationsupporting

confidence: 82%

“…At insemination, a semen dose containing 10 £ 10 9 spermatozoa in Beltsville Thawing Solution (BTS; Pursel & Johnson 1976) from two boars of proven fertility was used (Kaeoket et al 2002).…”

Section: Experimental Animals and Managementmentioning

confidence: 99%

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong¹,

Dalin²,

Sahlin³

et al. 2005

Reproduction

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“…Semen samples from each boar (one ejaculate from each boar) were collected using the gloved-hand technique [18,19]. During collection the semen was filtered through gauze, and only sperm-rich fractions were collected.…”

Section: Preparation Of Boar Spermatozoamentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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“…After incubation at 37°C for 20 min, a total of 200 spermatozoa were assessed (× 400) under a fluorescence microscope (Axioskop 40, Carl Zeiss Inc., Oberkochen, Germany). The nuclei of spermatozoa with intact plasma membranes were stained green with SYBR-14, while those with damaged membranes were stained red with EthD-1 [23][24][25][26][27]. Spermatozoa were classified into three types: viable, having intact plasma membranes (stained green); damaged, having damaged plasma membranes but an intact acrosome (stained red and green) and dead, having both plasma membranes and acrosomal damage stained red.…”

Section: Methodsmentioning

confidence: 99%

“…Water was provided ad libitum via a water nipple. A feed based on corn, soy bean and fish meal (15-16% protein) was given twice a day (approximately 3 kg/day).Semen samples from each boar (one ejaculate from each boar) were collected using the gloved-hand technique [23][24][25][26]. During collection, the semen was filtered through gauze, and only sperm-rich fractions were collected.…”

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confidence: 99%

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Kaeoket

Donto

Nualnoy

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to determine the effect of gamma-oryzanol-enriched -rice bran oil on the quality of cryopreserved boar semen. Ten boars provided semen of proven motility and morphology for this study. The semen was divided into three portions in which lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with 2 types of rice bran oils, at a gamma-oryzanol concentration of 0 mg/ml of lactose egg yolk (LEY) freezing extender (group A, control), 0.1 mg/ml(0.16 mMol) of freezing extender (group B) and 0.1 mg/ml of freezing extender (group C). Semen suspensions were loaded in medium straws (0.5 ml) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability and acrosomal integrity. There was a significantly higher percentage of progressive motility (34 versus 47.0 and 48.5, P<0.001), viability (35.5 versus 48.1 and 50.1, P<0.001) and acrosomal integrity (39.8 versus 50.8 and 54.9, P<0.001) in the gamma-oryzanolenriched rice bran oil-supplemented groups (groups B, C) than in the control group (group C), respectively. In conclusion, addition of gamma-oryzanol-enriched rice bran oil to LEY freezing extender is appropriated for improving the quality of frozen-thawed boar semen. Cryopreservation of boar spermatozoa offers an effective way to maintain their valuable genetic material and transport it between countries, thereby minimizing boar transportation. It has been demonstrated that certain processes in cryopreservation including elimination of seminal plasma by centrifugation and reduction in the temperature result in cold shock, owing to a decrease in quantity of antioxidants and an increase in reactive oxygen species (ROS) contents in the semen [6,8,43]. The ROS can lead to oxidative stress and lipid peroxidation of the sperm plasma membrane, which have detrimental effects on spermatozoa during cryopreservation, for instance, reduction in progressive motility, acrosomal integrity, sperm-oocyte fusion and fertilizing potential [1-3, 17, 41, 53].During the past decade, several studies have focused on supplementation of freezing extender for frozen boar semen with a variety of antioxidants (e.g., vitamin E, vitamin C, L-cysteine, glutathione, taurine, pyruvate, SOD, catalase) in an attempt to minimize the detrimental effects of ROS, which occur during the freezing process [7,10,16,23,26,38,39].Gamma-oryzanol, a phytosteryl ferulate mixture extracted from rice bran oil, has received a great deal of attention, because of its various significant health-promoting functions such as antioxidant activity, inhibition of lipoperoxidation by its scavenging activity [4], reduction in LDL cholesterol and induction of HDL cholesterol [40,41,48] and inhibition of platelet aggregation [13]. In humans, many studies have been reported on its potential implications as a UV-A filter in sunscreen cosmetics [14] and as a treatment for type 2 diabetes mellitus [12] and allergic reactions [3...

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The influence of pre- and post-ovulatory insemination on sperm distribution in the oviduct, accessory sperm to the zona pellucida, fertilisation rate and embryo development in sows

Cited by 27 publications

References 29 publications

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

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