The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing

Liu, Xiudong; Howard, Kenneth A.; Dong, Mingdong; Andersen, Morten Østergaard; Rahbek, Ulrik Lytt; Johnsen, Mads G.; Hansen, Otto; Besenbacher, Flemming; Kjems, Jørgen

doi:10.1016/j.biomaterials.2006.11.004

Cited by 360 publications

(304 citation statements)

References 38 publications

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“…As a successful example, the molecular weights of three kinds of chitosan were measured with the imaging function of AFM, and it was found that the molecular weight obtained in our experiment agreed well with that got by former method of Gel Permeation Chromatography [14] as expected.…”

Section: Introductionsupporting

confidence: 64%

Directly determining the molecular weight of chitosan with atomic force microscopy

Zhang¹,

Li²,

Zhang³

et al. 2016

Front Nanosci Nanotech

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Molecular weight will not only affect cytotoxicity, viscosity and other basic properties of molecules in physical and chemical fields, but also influence its efficiency of drug delivery in nanomaterial fields. It is of great importance to measure the molecular weight of a substance. In this study, we proposed a new method to directly determine the molecular weights of polymers with linear and repeated molecular structures. An empirical molecular weight formula of Chitosan with different molecular weights were chosen to value our new proposed method. Our results imply that the molecular weight of chitosan obtained with the new method is reasonable. Moreover, the distributions of molecular weight and molecular lengths can also be acquired at the same time with this novel method.

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Section: Introductionsupporting

confidence: 64%

Directly determining the molecular weight of chitosan with atomic force microscopy

Zhang¹,

Li²,

Zhang³

et al. 2016

Front Nanosci Nanotech

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show abstract

“…The influence of N/P ratio on the gene knockdown efficiency of chitosan 170 kDa (DA 16%) in H1299 human lung carcinoma cells was reported by Liu et al [90]. They found that the level of EGFP knockdown increased at higher N:P ratios (50 and 150) in comparison to lower N:P ratios (2 and 10), and the nanoparticles formed at higher N:P (50 and 150) showed the greatest level (80%) of EGFP knockdown.…”

Section: Influence Of Concentrationmentioning

confidence: 71%

“…Apart from the influence of the chitosan concentration on its antimicrobial behavior, this parameter determines the properties of chitosan-based formulations for gene delivery systems [51,89,90]. It was established that the particle size of the chitosan/siRNA complex prepared by simple complexation increased when the concentration of chitosan was raised [51].…”

Section: Influence Of Concentrationmentioning

confidence: 99%

“…The comparative positive value of the surface charge (zeta potential) of chitosan-siRNA complexes also increased with the rising concentration of chitosan at a constant siRNA concentration. The transfection efficiency of the polyelectrolyte obtained depends on the mixing molar stoichiometry of DNA to chitosan (N/P ratio) [51,89,90]. Sajomsang et al reported that the highest gene transfection efficiencies of all N-aryl chitosan derivative/DNA complexes were observed at N/P ratios 5 due to the smallest particle size (95-124 nm) [89].…”

Section: Influence Of Concentrationmentioning

confidence: 99%

“…Antimicrobial DQ ↑ at pH 7.2, no effect at acidic conditions [104] Adjuvanticity DQ no effect [106] Antithrombin DS ↑ [109] Acid-blinding capacity DS ↑ up to 25% [111] Immune enhancing effect pH < 4 or pH > 10 ↑ [76] Calcium/cobalt blinding capacity pH ↑ [82] Transfection efficiency C↓ [51,89,90] Gene knockdown efficiency C ↓ [90] ↑ directly proportion to property; ↓ inversely proportional to property…”

Section: Biological Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

et al. 2011

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Abstract:The physicochemical nature of chitin and chitosan, which influences the biomedical activity of these compounds, is strongly related to the source of chitin and the conditions of the chitin/chitosan production process. Apart from widely described key factors such as weight-averaged molecular weight (M W ) and degree of N-acetylation (DA), other physicochemical parameters like polydispersity (M W /M N ), crystallinity or the pattern of acetylation (P A ) have to be taken into consideration. From the biological point of view, these parameters affect a very important factor-the solubility of chitin and chitosan in water and organic solvents. The physicochemical properties of chitosan solutions can be controlled by manipulating solution conditions (temperature, pH, ionic strength, concentration, solvent). The degree of substitution of the hydroxyl and the amino groups or the degree of quaternization of the amino groups also influence the mechanical and biological properties of chitosan samples. Finally, a considerable research effort has been directed towards developing safe and efficient chitin/chitosan-based products because many factors, like the size of nanoparticles, can determine the biomedical characteristics of medicinal products. The influence of these factors on the biomedical activity of chitin/chitosan-based products is presented in this report in more detail. OPEN ACCESSPolymers 2011, 3 1876

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Discovery of New SiRNA Delivery Agents

et al. 2010

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The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing

Cited by 360 publications

References 38 publications

Directly determining the molecular weight of chitosan with atomic force microscopy

Directly determining the molecular weight of chitosan with atomic force microscopy

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

Discovery of New SiRNA Delivery Agents

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