The influence of pH and concentration of mucins on diesel exhaust particles (DEPs) transport through artificial mucus

Penconek, Agata; Moskal, Arkadiusz

doi:10.1016/j.jaerosci.2016.09.001

Cited by 8 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, along the gastrointestinal tract, the pH fluctuates between pH 1 and 8. [58,59] The pH of the mucus-containing layer which lines the airway epithelium, in both normal and CF airways, ranges between pH 5.5 - 6.7 in the nasal mucosa, and is ∼ pH 7.0 in the bronchia/lungs. [60,61] Interestingly, neonates with CF reportedly have a more acidic pH (pH 5.2) than non-CF neonates (pH 6.4).…”

Section: Resultsmentioning

confidence: 99%

Exploring Mechanisms of Lipid Nanoparticle-Mucus Interactions in Healthy and Cystic Fibrosis Conditions

Tafech,

Rokhforouz,

Leung

et al. 2024

Preprint

View full text Add to dashboard Cite

Mucus forms the first defense line of human lungs, and as such hampers the efficient delivery of therapeutics to the underlying epithelium. This holds particularly true for genetic cargo such as CRISPR-based gene editing tools which cannot readily surmount the mucosal barrier. While lipid nanoparticles (LNPs) emerged as versatile non-viral gene delivery systems that could help overcome the delivery challenge, many knowledge gaps remain, especially for diseased states such as cystic fibrosis (CF). This study provides fundamental insights into Cas9 mRNA or ribonucleoprotein-loaded LNP-mucus interactions in healthy and diseased states by assessing the impact of the genetic cargo, mucin sialylation, mucin concentration, ionic strength, pH, and polyethylene glycol (PEG) concentration and nature on LNP diffusivity leveraging experimental approaches and Brownian dynamics simulations. Taken together, this study identifies key mucus and LNP characteristics that are critical to enabling a rational LNP design for transmucosal delivery.

show abstract

Section: Resultsmentioning

confidence: 99%

Exploring Mechanisms of Lipid Nanoparticle-Mucus Interactions in Healthy and Cystic Fibrosis Conditions

Tafech,

Rokhforouz,

Leung

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…This model was also used in our previous work to determine the impact of diesel exhaust particles on mucus rheology (Penconek and Moskal 2016 ).…”

Section: Methodsmentioning

confidence: 99%

“…The apparent viscosity of mucus decreases while pH is increasing (Bansil and Turner 2006 ). The viscosity of mucus also decreases due to the presence of acidic pollutants (Holma and Hegg 1989 ), diesel exhaust particles (Penconek and Moskal 2016 ) or smoking (Kollerstrom et al 1977 ), but increases when immunoglobulins appear in it (Girod et al 1992 ). The viscoelastic properties of mucus, as well as chemical composition, are dependent on place of secretion, age, sex, physical activity or even atmospheric conditions.…”

Section: Introductionmentioning

confidence: 99%

The effect of desert dust particles on rheological properties of saliva and mucus

Penconek

Michalczuk

Sienkiewicz

et al. 2019

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Transported desert dust particles (TDDP) are soil particles suspended in the air. Being spread all over the globe by the winds, TDDP affect animals, including humans, plants and other organisms not only in the areas of their emission. In humans, TDDP are responsible for diseases of the respiratory (e.g. asthma) and circulatory (e.g. heart failure) systems and they also act directly on the epithelium and its mucus layer after deposition in the mouth and respiratory system. The aim of the study was to determine the influence of TDDP on the rheology of mucus and saliva, and thus on their functioning. The artificial mucus and saliva, as well as Arizona TDDP, were used in experiments. The rheological properties of TDDP were determined with the use of an oscillatory rheometer, at various temperatures and in the presence of different amount of TDDP. Moreover, the diffusion time of the marker (rhodamine B) throughout mucus with desert dust particles was examined. The obtained results demonstrate that the presence of TDDP in the saliva and mucus model increases their apparent viscosity. The concentration of particles is positively correlated with the increase of viscosity. However, it has not been demonstrated that the presence of TDDP in mucus significantly influenced the diffusion of a fluorescent marker throughout the mucus. The presence of TDDP in the saliva and mucus may interfere with their moisturising function, and cause difficulties in swallowing by increasing the viscosity of mucus and saliva. Moreover, increased viscosity of mucus may cause problems with its ability to pass to the upper respiratory tracts, which may lead to a general discomfort or local inflammation.

show abstract

“…Two types of diesel oil were used to generate the DEPs (Ecodiesel [cetane number 51] and Verva [cetane number 55]). The DEPs were collected in a 50 mm diameter glass Petri dish, similar to those described elsewhere [37], and stored in the dark at 4 °C.…”

Section: Soot Aggregates-diesel Exhaust Particles (Deps)mentioning

confidence: 99%

The Influence of Exogenous Particles on Saliva Rheology

Penconek,

Przekop,

Michalczuk

et al. 2024

Processes

View full text Add to dashboard Cite

This study aimed to investigate the effect of exogenous nanoparticles on the rheological properties of artificial saliva. There are four reasons for undertaking this type of research: Firstly, the number of solid particles of various origins present in the air is still high. Secondly, nanoparticles (including silver and gold nanoparticles) are increasingly used in food packaging and can migrate into food. Thirdly, saliva is the first biological fluid that comes into contact with exogenous particles. Finally, the function of saliva is also closely related to its rheological properties. Due to the remarkable properties of nano-objects, nanoparticles of various origins in the body may cause effects that have not been realised until now. Therefore, each type of nanoparticle must be tested in terms of its impact on the body/body fluid. We used silver and gold nanoparticles because they are used in the food industry, and diesel exhaust particles because they are standard components of air pollution. The effect of various nanoparticles (e.g., their size and shape) on the rheology of saliva at two temperatures was investigated. The constants of the power law constitutive equation were also estimated. Studies showing the impact of nanoparticles on the rheology of body fluids are rare because it is one of the less obvious ways of their affecting the human body. However, the results show that nanoparticles are not neutral to the biological fluid, which may translate into a change in its properties and thus disturb its functions.

show abstract

The influence of pH and concentration of mucins on diesel exhaust particles (DEPs) transport through artificial mucus

Cited by 8 publications

References 26 publications

Exploring Mechanisms of Lipid Nanoparticle-Mucus Interactions in Healthy and Cystic Fibrosis Conditions

Exploring Mechanisms of Lipid Nanoparticle-Mucus Interactions in Healthy and Cystic Fibrosis Conditions

The effect of desert dust particles on rheological properties of saliva and mucus

The Influence of Exogenous Particles on Saliva Rheology

Contact Info

Product

Resources

About