The influence of methyl jasmonate, cholesterol and l‐arginine on solasodine production in hairy root culture of Solanum mammosum

Abstract

The increasing demand of diosgenin for high-revenue synthesis of steroid hormones by the pharmaceutical industries has driven researchers to look for other alternatives. Solasodine which was reported to be present in Solanum mammosum is known to be a potential source. The present study highlighted that added methyl jasmonate, cholesterol and l-arginine into the modified liquid full-strength Murashige and Skoog (MS) medium (with ammonium to nitrate ratio 10.3 mM: 39.4 mM, and 4% (w/v) sucrose) could influence the solasodine production in the hairy roots of S. mammosum. The findings showed that both hairy root line-ATCC31798 and line-A4 (which were separately induced by Agrobacterium rhizogenes strain ATCC31798 and A4) acquired solasodine productivity of 4.5 mg/g dry weight roots with average dry biomass of 190 mg after 32 days culture, when using 50 mg fresh weight roots as initial inoculum size, with 100 mM cholesterol, 1000 μM l-arginine and 300 μM methyl jasmonate added simultaneously into the culture medium on day 20 of culture. The amount of solasodine obtained was five times higher than those without both the elicitor and precursor treatment. The improved solasodine production with a high-biomass growth could reduce the production cost of steroid synthesis in the long run.

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

“…The application of optimum concentration of MJ (300 µM) along with the precursors such as cholesterol (100 mM) and L‐arginine (1000 µM) supplemented modified liquid MS medium on 20‐days‐old HRCs (hairy root line‐ATCC31798 and line‐A4) of Solanum mammosum improved solasodine productivity five‐fold (4.5 mg g −1 ) with a high‐biomass accumulation (in average 190 mg DW) compared to control HRCs cultured without both the elicitor and precursor treatment .…”

Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy

“…Organ cultures, including shoot cultures, are often used in studies on feeding experiments e.g. [9,10,14,15,[27][28][29]. L-phenylalanine is one of frequently used precursors in feeding experiments [10].…”

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

“…Even so, the production levels can be further improved by the application of elicitors in the hairy root culture systems. Several studies have shown that enhanced production of different classes of metabolites can be achieved in hairy root cultures of solanaceous plants of Anisodus acutangulus , Atropa acuminata , A. baetica , A. belladonna , Brugmansia candida , Datura metel , D. stramonium , Hyoscyamus albus , H. muticus , H. reticulatus , Solanum mammosum , S. myriacanthum , S. trilobatum , and Withania somnifera , upon elicitation ( Mehmetoğlu and Curtis, 1997 ; Kuroyanagi et al., 1998 ; Pitta-Alvarez and Giulietti, 1998 ; Jacob and Malpathak, 2005a ; Jacob and Malpathak, 2005b ; El Jaber-Vazdekis et al., 2008 ; Doma et al., 2012 ; Kai et al., 2012a ; Shilpha et al., 2015 ; Ooi et al., 2016 ; Srivastava et al., 2016 ; Moharrami et al., 2017 ; Shakeran et al., 2017 ; Amdoun et al., 2018 ; Moradi et al., 2020 ; Fattahi et al., 2021 ) ( Supplementary Table 1 ).…”