The influence of maintaining the correct whole blood‐to‐anticoagulant ratio during donation on the quality of leukoreduced whole blood

Karger, Ralf; Lukow, Christian; Kretschmer, V.

doi:10.1111/j.1537-2995.2010.03030.x

Cited by 2 publications

(3 citation statements)

References 33 publications

(58 reference statements)

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“…The ATP concentration was measured enzymatically using a commercial test kit (Rolf Greiner BioChemica, Flacht, Germany). Details of the different methods are reported elsewhere [14].…”

Section: Laboratory Investigationsmentioning

confidence: 99%

“…In this study, 2 × 20 leukocyte-depleted whole blood (LD-WB) units were investigated in a randomized controlled fashion [14]. In 1 group, an anticoagulant (citrate phosphate dextrose adenine 1; CPDA-1) was continuously added to 500 ml donated whole blood via a roller pump attached to scales, using a new blood drawing device, the 'Automat for Blood Collection' (MacoPharma ABC ® , MacoPharma, Langen, Germany).…”

Section: Blood Collectionmentioning

confidence: 99%

“…Since red cell flexibility or deformability are major determinants of red cell viability [13], the aim of this study was to investigate, in addition to established biochemical markers, rheological properties of red cells at different shear rates in leukocyte-depleted whole blood over a 7-week storage period. This study is a post hoc analysis of a randomized experiment comparing different blood collection methods [14].…”

Section: Introductionmentioning

confidence: 99%

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Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Karger

Lukow

Kretschmer³

2012

Transfus Med Hemother

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KeywordsATP content · Deformability · Quality · Red cell storage · Storage lesion · In vivo survival · Whole blood Summary Background: Storage duration of red cells has been associated with increased morbidity and mortality following transfusion. This association has been attributed to the loss of deformability of stored red cells leading to deterioration of microvascular perfusion. ATP content is considered a critical determinant of the deformability of stored red cells. Methods: ATP content and deformability were determined after storage for up to 49 days in 40 leukocyte-depleted whole blood units. Red cell deformability was determined using a laser-assisted optical rotational cell analyzer (LORCA ® ) employing shear stress (SS) ranging from 0.3 to 30 Pa. Deformability was expressed as the elongation index (EI). EI was correlated with ATP content. Results: ATP content decreased from 3.5 to 1.7 mol/g hemoglobin. EI increased from 0.03 to 0.05 at an SS of 0.

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“…The ATP concentration was measured enzymatically using a commercial test kit (Rolf Greiner BioChemica, Flacht, Germany). Details of the different methods are reported elsewhere [14].…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Section: Blood Collectionmentioning

confidence: 99%