The influence of macronutrients and some other factors on growth of Phalaenopsis hybrid seedlings in vitro

Hinnen, M.G.J.; Pierik, R.L.M.; Bronsema, F.B.F.

doi:10.1016/0304-4238(89)90054-x

Cited by 20 publications

(11 citation statements)

References 4 publications

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“…So, as far as the level of Nitrogen was increased, fewer roots were emitted in number and in length. A similar effect was observed in vitro cultivation of Phalaenopsis hybrids, where increases in NH + 4 and NO − 3 concentration decreased root growth (HINNEN et al, 1989) and, by the observed associations, it can be supposed that, Nitrogen offer for cuttings of mature stalks may depress root emission.…”

Section: Resultssupporting

confidence: 74%

Stems age, nitrogen fertilizer and salicylic acid application in cutting induction of noble dendrobium orchid of the Yamamoto series cultivars

Soccol

Venturieri

Pedrotti

2017

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Propagation of noble dendrobium orchid (Dendrobium nobile Lindl.) by cutting was studied in two experiments. In the first experiment we evaluated the effect stem age on propagation success: mature stems -from already bloomed stems; and young stems -yet to bloom; and Nitrogen fertilizer application, from two sources: as Nitrate and Ammonium (respectively as Calcium Nitrate at concentrations of: 5.81 gL -1 ; 11.61 gL -1; 17.42 gL -1; and Urea at concentrations of 2.00 gL -1 ; 4.00 gL -1 and 6.00 gL -1 plus control treatments). We evaluated the following parameters: the number of cuttings stalks that launched shoots and/or roots, vigor, number of roots per plant and root length per plant. Factorial analysis of variance (stems age x source of Nitrogen; and age of stem x Nitrogen level) was applied using a Generalized Linear Model (GLM) approach. Where significant differences were observed, averages were compared using post-hoc tests (Tukey). Propagation success was higher using cuttings from mature stems (60.2%), a value 1.6 times higher than obtained with stem cuttings from young stems (38.0%). Application of Nitrogen, in both forms, did not influence any of the evaluated parameters. In the second experiment we treated cuttings from mature stems with Salicylic acid in 3 concentrations (0.10 mM; 0.50 mM; 1.00 mM and plus a control treatment). Evaluated parameters included proportion of cuttings stalks that launched shoots and/or roots, leaf length, root length, and number of roots per stem cutting. Factorial analysis of variance was applied with post-hoc tests. Application of 0.50 mM of Salicylic acid increased the proportion of cuttings stalks that launched shoots and/or roots by 20.5% relative to the control treatment. Keywords: Dendrobium nobile, propagation, small farmers, floriculture, elicitor molecule RESUMO Idade dos ramos, aplicação de fertilizantes nitrogenados e ácido salicílico, na estaquia da orquídea olho-de-boneca em cultivares da série Yamamoto A propagação da orquídea olho-de-boneca (Dendrobium nobile Lindl.) por estaquia, foi estudada em dois experimentos: no primeiro foi avaliado o efeito de duas idades de hastes utilizadas para as estacas: maduras -que já haviam florescido; e jovensque não haviam florescido ainda; e aplicação de fertilizantes nitrogenados, oriundos de duas fontes: na forma de nitrato e na de amônia (respectivamente como nitrato de cálcio nas concentrações de 5,81 gL -1 ; 11,61 gL -1; 17,42 gL -1 ; e uréia nas concentrações de 2,00 gL -1 ; 4,00 gL -1 e 6,00 gL -1 mais tratamentos controle. Os parâmetros avaliados foram: estacas que lançaram rebentos e/ou raízes, vigor, número de raízes e comprimento de raízes/por planta. A análise de variância fatorial foi aplicada (idade dos ramos x fonte de nitrogênio; e idade dos ramos x nível de nitrogênio) usando o Modelo Linear Generalizado (MLG). Quando diferenças significativas foram observadas, as medias foram comparadas pelo teste de Tukey. O melhor resultado foi obtido usando estacas oriundas de ramos maduros (60,2%), valor 1,6 ...

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Section: Resultssupporting

confidence: 74%

Stems age, nitrogen fertilizer and salicylic acid application in cutting induction of noble dendrobium orchid of the Yamamoto series cultivars

Soccol

Venturieri

Pedrotti

2017

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“…Good growth and development of Phalaenopsis plantlets in vitro were obtained when culture media were supplemented with 0.2% (w/v) AC (Hinnen et al, 1989;Ernst, 1994). Activated charcoal reduce the phenolic exudates, the beneficial effects of AC could be due to positive stimulation of many development processes (Van Winkle and Pullman, 2006) and phenolic compounds absorbtion.…”

Section: Anti-contaminantsmentioning

confidence: 99%

A Review on: In Vitro Cloning of Orchids

Sarmah¹,

Kolukunde²,

Sutradhar³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…In the following 14 days (80 days after inoculation), plants with two leaves and one to two roots appeared. Hinnen et al (1989) noted the importance of nitrogen in media, which influences growth. They also mentioned that strong shoot growth reflects weaker root development, which they define as the ratio shoot/roots.…”

Section: Morphological Stagesmentioning

confidence: 99%

“…A nitrate form of nitrogen is more accessible to a plant, but only when the plant reaches the developmental stage where enzymes started to function. The addition of an ammonium form of nitrogen as well is therefore recommended (Hinnen et al, 1989).…”

Section: Morphological Stagesmentioning

confidence: 99%

Asymbiotic seed germination of Phalenopsis Blume orchids after hand pollination

Lesar¹,

Čeranič²,

Kastelec³

et al. 2012

Acta Agriculturae Slovenica

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Seven commercial hybrids of Phalaenopsis were included in an asymbiotic germination experiment. Plants differed in size and color of flowers and number of inflorescences and flowers. A total of 109 flowers were included of which 60 or 55% were cross-pollinated and reciprocally pollinated. Seed capsules developed on 46 (76.7%) fertilized flowers. Of these, 38 (82.6%) produced seeds as a result of 19 combinations of cross crossing and 19 combinations of reciprocal crossing. Seeds were surface sterilized using 1.6% dichloroisocyanuric acid and inoculated in Petri dishes on commercial media Sigma P1056, in two repeats. There were 23.7% contaminated Petri dishes with seeds after sterilization. The highest average percentage of protocorms (43.1%) developed in cross crossing of plants 3x7, which statistically significantly differed (p < 0.001) from all other combinations of cross crossing except cross crossing 2x6 (30.6%). Crossing 2x6, 1x6 (8.1%) and 1x5 (5.5%) overlap and there were no statistically significant differences between them. There were no statistically significant differences among all of the remaining crossings. There was an overlap of groups among combinations of cross crossing with small flowers x big flowers and reciprocal crossing of big flowers x small flowers. In successful crossing combinations, first plants with two leaves and one or two roots developed only 80 days after seed inoculation on media. Plant no. 4 with the smallest green-yellow flowers was not compatible with any test plants. Seed capsules without seeds developed when plant 4 was a female plant and flowers just fell off when plant 4 was a male plant. Največji odstotek nastalih protokormov (43,1%) je bil dobljen pri navzkrižnem križanju rastlin 3x7, ta odstotek se statistično značilno (p < 0.001) razlikuje od ostalih kombinacij navzkrižnih križanj, razen navzkrižnega križanja 2x6 (30,6%). Križanja 2x6, 1x6 (8.1%) in 1x5 (5.5%) se prekrivajo in med njimi ni statistično značilnih razlik. Prav tako ni statistično značilnih razlik med ostalimi kombinacijami križanja. Med skupinami navzkrižnih križanj rastlin z malimi cvetovi x rastlin z večjimi cvetovi in recipročnih križanj rastlin z velikimi cvetovi x malimi cvetovi pride do medsebojnega prekrivanja in med njimi ni statistično značilnih razlik. Pri uspešnih kombinacijah križanj so že po 80 dneh inokulacije semen na gojišče nastajale prve rastline z dvema listoma in eno ali dvema koreninama. Rastlina z oznako 4 z najmanjšimi, zeleno rumenimi cvetovi ni bila kompatibilna z nobeno od vključenih rastlin v križanja. V primerih, ko je bila materina rastlina, so nastale semenske glavice brez semen, v primerih, ko je bila očetova rastlina, so cvetovi po oprašitvi odpadli.

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The influence of macronutrients and some other factors on growth of Phalaenopsis hybrid seedlings in vitro

Cited by 20 publications

References 4 publications

Stems age, nitrogen fertilizer and salicylic acid application in cutting induction of noble dendrobium orchid of the Yamamoto series cultivars

Stems age, nitrogen fertilizer and salicylic acid application in cutting induction of noble dendrobium orchid of the Yamamoto series cultivars

A Review on: In Vitro Cloning of Orchids

Asymbiotic seed germination of Phalenopsis Blume orchids after hand pollination

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