A Review on: In Vitro Cloning of Orchids

Sarmah, Dipika; Kolukunde, Swathi; Sutradhar, Monoj; Singh, Brijesh Kumar; Mandal, Tapas K.; Mandal, Nirmal

doi:10.20546/ijcmas.2017.609.235

Cited by 19 publications

(21 citation statements)

References 21 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the Orchidaceae is the largest among all plant species and has very decorative plants, species propaga-tion is important, and biotechnological in vitro methods are essential (Sarmah et al, 2017). Cymbidium has multiple hybrids and cultivars, so propagation protocols for each can be extremely diverse.…”

Section: Discussionmentioning

confidence: 99%

“…and CKs such as BA, KIN, and TDZ, along with auxins such as IAA and NAA, are involved in this process (Ghosh et al, 2014;Sarmah et al, 2017). In Cymbidium micropropagation, CKs are generally used to promote PLB proliferation, axillary branching, and shoot elongation (Teixeira da Silva et al, 2006b), although the optimal CK concentration varies with the genotype used while higher CK concentrations inhibit shoot growth (Paek and Yeung, 1991;Chang and Chang, 2000a;Lu et al, 2001).…”

Section: Pgrs Play a Major Role In Cymbidium Organogenesismentioning

confidence: 99%

“…KIN, unlike other CKs, promoted an increase in PBL size ( Table 2). The presence of CKs and auxins in basal medium is a common practice for micropropagating orchids (Teixeira da Silva, 2013a;Sarmah et al, 2017). Broadly, the micropropagation of several orchid species can be achieved when full-strength MS is supplemented with NAA (0.1-5.0 mg @ dm 3 ), BA (0.1-2.5 mg @ dm 3 ), or TDZ (4.54 -18.16 mg @ dm 3 ) (Sarmah et al, 2017).…”

Section: Pgrs Play a Major Role In Cymbidium Organogenesismentioning

confidence: 99%

“…The Orchidaceace is the largest plant family with 25 000-35 000 species (Sarmah et al, 2017), and the second most popular cut flower and potted floricultural crop (Albert et al, 2010;Hossain et al, 2010). Detailed global market statistics for the floricultural industry over the past decade are not easily available.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

Лукаткин¹,

Mokshin²,

Bol'shakova³

et al. 2019

bta

View full text Add to dashboard Cite

Hybrid Cymbidium is an important ornamental resource as an orchid pot plant and in cut flower markets, so any protocol that can lead to its improved productivity in vitro is always welcome. Based on a cultivar "Memoria Amelia Earhart" that has not been yet studied, the effects on in vitro organogenesis in response to diluted Murashige and Skoog (MS) medium inorganics and plant growth regulators [PGRs: Epin-Extra ® (24-epibrassinolide, 24-EpiB), Ribav-Extra ® (RE), kinetin (KIN), thidiazuron (TDZ), 6-benzyladenine (BA), and indole-3-acetic acid (IAA)], either alone or as combinations between cytokinins and IAA, were assessed. Whole protocorm-like bodies (PLBs) served as the initial explant source. While full-strength MS medium benefited shoot growth, half-strength MS was optimal for rooting and PLB formation. The optimum concentrations of 24-EpiB were 10 4 % and 10 5 % for shoot growth and 10 6 % for PLB multiplication, and the maximum shoot growth was induced by RE at 10 4 % and BA at 4.4 μM or 13.3 μM. The simultaneous application of a cytokinin and IAA in MS had the greatest impact on PLB-related parameters. The number of PLBs per explant was improved by 11.6 μM KIN and 2.9 μM IAA, while 0.5 μM KIN and 2.9 μM IAA improved PLB size. The number of regenerated shoots was maximum in MS medium supplemented with 10 9 μM TDZ and 2.9 μM IAA, whereas the longest shoots formed in MS medium supplemented with 2.2 μM BA and 2.9 μM IAA. Using well-explored and unexplored PGRs, we devised an effective in vitro PLB-to-plantlet regeneration protocol for the hybrid Cymbidium "Memoria Amelia Earhart".

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Pgrs Play a Major Role In Cymbidium Organogenesismentioning

confidence: 99%

Section: Pgrs Play a Major Role In Cymbidium Organogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

Лукаткин¹,

Mokshin²,

Bol'shakova³

et al. 2019

bta

View full text Add to dashboard Cite

show abstract

“…The Orchidaceae is among the largest families of angiosperms, and is composed of approximately 736 genera and about 28,000 species [1]. Among the diverse genera in the family, Dendrobium, Phalaenopsis, Oncidium, and Cymbidium are important floricultural crops in Asian countries [2]. In particular, Cymbidium is economically important in northeastern Asia, including Korea, China, and Japan [3].…”

Section: Introductionmentioning

confidence: 99%

Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

Kim

Ahn

et al. 2020

Plants

View full text Add to dashboard Cite

Ionizing radiation combined with in vitro tissue culture has been used for development of new cultivars in diverse crops. The effects of ionizing radiation on mutation induction have been analyzed on several orchid species, including Cymbidium. Limited information is available on the comparison of mutation frequency and spectrum based on phenotypes in Cymbidium species. In addition, the stability of induced chimera mutants in Cymbidium is unknown. In this study, we analyzed the radiation sensitivity, mutation frequency, and spectrum of mutants induced by diverse γ-ray treatments, and analyzed the stability of induced chimera mutants in the Cymbidium hybrid cultivars RB003 and RB012. The optimal γ-irradiation conditions of each cultivar differed as follows: RB003, mutation frequency of 4.06% (under 35 Gy/4 h); RB012, 1.51% (20 Gy/1 h). Re-irradiation of γ-rays broadened the mutation spectrum observed in RB012. The stability of leaf-color chimera mutants was higher than that of leaf-shape chimeras, and stability was dependent on the chimera type and location of a mutation in the cell layers of the shoot apical meristem. These results indicated that short-term γ-irradiation was more effective to induce mutations in Cymbidium. Information on the stability of chimera mutants will be useful for mutation breeding of diverse ornamental plants.Plants 2020, 9, 546 2 of 11 breeding [6]. However, development of a new Cymbidium cultivar by means of cross-breeding is time-consuming because of the long vegetative growth stage.Mutation breeding techniques using physical mutagens (e.g., γ-rays, X-rays, and ion particles) or chemical mutagens (e.g., ethyl methanesulfonate, N-nitroso-N-methylurea, and colchicine) have been widely used to develop mutant cultivars in diverse plant species, including food and ornamental crops. In 226 plant species, 3308 mutants, including two Cymbidium mutants, that were predominantly (77.5%) developed using physical mutagens have been registered in the Mutant Variety Database of the joint Food and Agriculture Organization of the United Nations/International Atomic Energy Agency [7]. Among the diverse ornamental plants registered on the database, the most numerous mutants are those of chrysanthemum (Chrysanthemum spp., 274 mutants) followed by rose (Rosa spp., 67 mutants), dahlia (Dahlia spp., 36 mutants), alstroemeria (Alstroemeria spp., 35 mutants), streptocarpus (Streptocarpus spp., 30 mutants), and carnation (Dianthus caryophyllus, 28 mutants) [7]. The combination of treatment with a physical mutagen and in vitro tissue culture has been used to shorten the breeding period of orchids [8,9]. Thus, integration of γ-irradiation and in vitro tissue culture may be an efficient procedure for development of mutant cultivars in Cymbidium.The treatment of seeds, buds (tip/node cuttings), callus, and rhizomes with a mutagen induces chimeras in M 1 plants because mutations are induced in individual cells and regenerated shoots are recovered from pre-existing multicellular meristems [10]. In seed-propagated pl...

show abstract

Insights into the In Vitro Approaches for the Production of Secondary Metabolites Towards The Conservation of Medicinal Orchids

Singh

Kumaria

2023

Sustainable Development and Biodiversity

View full text Add to dashboard Cite

A Review on: In Vitro Cloning of Orchids

Cited by 19 publications

References 21 publications

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

Effects of inorganic salts concentration and alternative plant growth regulators on the in vitro organogenesis of a new hybrid Cymbidium

Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

Insights into the In Vitro Approaches for the Production of Secondary Metabolites Towards The Conservation of Medicinal Orchids

Contact Info

Product

Resources

About