The influence of light quality on phenolic acid and biflavonoid production in Anacardium othonianum Rizz. seedlings grown in vitro

Abstract

Anacardium othonianum Rizz., popularly known as the Cerrado cashew tree, is widely used in medicine due to its therapeutic properties, including its antioxidant, anti-inflammatory, and antidiarrheal activities. Light is an important regulator of the growth, development of plants differentiation, and metabolism. Considering the influence of light on the production of phytochemicals, the aim of the present study was to evaluate production of gallic and 3,4-dihydroxybenzoic acids and amentoflavone in A. othonianum seedlings grown in vitro exposure to white, blue, green, red, and yellow light. The seedlings were evaluated with respect to the length of the aerial parts and the number of leaves, at 0, 20, and 40 days of treatment. The fresh and dry weights of the aerial parts and the levels of phenolic acid and biflavonoid were evaluated at 40 days via high-performance liquid chromatography with a diode array detector (HPLC-DAD). Regardless of the light wavelength used for the growth, the largest length of the aerial parts (5.54 cm) and an increase in the number of leaves (2.86-fold) were observed at 40 days. The largest accumulations of fresh weight (325.5 mg and 335.1 mg per seedling) and dry weight (83.3 mg and 84.5 mg per seedling) were observed in seedlings grown under red and yellow light, respectively. Relatively higher levels of gallic acid (0.66 mg.g -1 dry weight) and amentoflavone (1.41 mg.g -1 dry weight) were found in seedlings grown under blue light. In contrast, the 3,4-dihydroxybenzoic acid levels of the seedlings under red light increased (8.22 mg.g -1 dry weight).

Ciências Biológicas: Tendências temáticas, realidades e virtualidades

“…Na cultura in vitro de calos e brotos de várias espécies, as diferentes qualidades de luz fornecidas por lâmpadas fluorescentes têm sido utilizadas para estimular a fotossíntese, morfogênese e a biossíntese de compostos químicos de interesse (SZOPA; EKIERT, 2016TO;GAZOLLA et al, 2017;PEDROSO et al, 2017).…”

Plantas medicinais e suas potencialidades

“…As folhas e cascas tem atividade antidiarréica e expectorante. O óleo extraído das sementes é usado para eliminar manchas e verrugas, e o pseudofruto é considerado anti-sifilítico (SOUZA et al, 2017;Costa et al, 2006;GAZOLLA et al, 2017).…”

Growth, ferulic acid synthesis, and histochemistry of calli of Pouteria caimito (Ruiz & Pav.) Radlk under different light qualities

“…Blue and red light-emitting diodes increase the total phenolic content and chlorogenic acid content in pea sprouts (Liu et al, 2016). Although radiation, such as violet, green, yellow, and orange light, is only related to secondary beneficial effects on plant growth and development (Gazolla et al, 2017), it was Research, Society and Development, v. 9, n. 8, e556985822, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i8.5822 found, for example, that in callus culture of Artemisia absinthium L., the green spectrum favors the production of total phenolics, flavonoids, chlorophylls, and antioxidant activity (Tariq et al, 2014).…”

“…There are no reports on the influence of light quality on the growth or secondary metabolism of P. caimito, especially under in vitro conditions. Studies involving spectral adequacy can support relevant strategies to optimize plant growth and the production of secondary metabolites (Gazolla et al, 2017). Given the above, the objective of this study was to investigate the influence of light quality on the growth, histochemistry, and ferulic acid content in callus culture of P. caimito.…”