The influence of L‐proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation

Abstract

Background: This study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA).Objectives: Therefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation.Methods: Thirty semen samples of three buffalo bulls were diluted in Tris-egg yolk extender and divided into 12 equal groups including control (

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

“…A CASA system (Test Sperm 3.2; Videotest, St. Petersburg, Russia) was used to measure total motility (%), progressive motility (%), curvilinear velocity (VCL; µm s –1 ), straight-line velocity (VSL; µm s –1 ), average path velocity (VAP; µm s –1 ), straightness (STR; %), linearity (LIN; %) amplitude of lateral head displacement (ALH; µm s –1 ), and beat-cross frequency (BCF; Hz) (Table 1 ; [ 37 ]). Ten microliters of semen were used to evaluate at least 500 spermatozoa in 5 microscopic fields [ 38 ].…”

“…In this sense, sperm showing cytoplasmic remnants as well as other obvious defects were classified as abnormal sperm. For each sample, 200 sperm were examined at a magnification of 400× and the results presented as a percentage [ 38 ].…”

“…A TAC kit (Naxifer™; Navand Salamat Company, Urmia, Iran) was used to quantify of TAC in testis [ 38 ]. The TAC values ​​were given in nmol/mg protein.…”

“…A GPx kit (Nagpix™, Navand Salamat Company, Urmia, Iran) was used to calculate the GPx level in testis [ 38 ]. The GPx value was given in mU/g protein.…”

Protective effects of the bioactive peptide from maggots against skin flap ischemia‒reperfusion injury in rats

“…Enzymes are affected from ROS and LPO production, so superoxide dismutase (SOD) and catalase (CAT) are antioxidant enzymes that play an essential role against oxidative stress [ 9 ]. Therefore, antioxidants could protect cells against damage in flap transplantation and other related diseases [ 10 , 11 ]. The TLR4/NF-κB and Nrf2 pathways are suggested to be related to intracellular signaling during I/R injury [ 12 , 13 ].…”