The influence of ketone bodies and glucose on interferon, tumor necrosis factor production and NO release in bovine aorta endothelial cells

Zdzisińska, Barbara; Filar, J; Paduch, Roman; Kaczor, Józef; Lokaj, I; Kandefer‐Szerszeń, Martyna

doi:10.1016/s0165-2427(00)00175-6

Cited by 14 publications

(13 citation statements)

References 20 publications

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“…A similar finding has also been recorded in experimentally induced ketosis in rabbits (Celik and Karagul 2005). The significant increase of NO level was also similar to with those obtained by ZdzisinÂska et al (2000) and Noh et al (2006). NO is one of the most widespread signaling molecules, which participates in virtually cellular function in the body (Abou-Zeina et al 2009).…”

Section: Discussionsupporting

confidence: 80%

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Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level

Youssef

El-Khodery

El-Deeb

et al. 2010

Trop Anim Health Prod

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As little is known about the oxidant/antioxidant status in buffalo with ketosis, the present study was delineated to assess the oxidative stress level associated with clinical ketosis in water buffalo. A total of 91 parturient buffalo at smallholder farms were studied (61 suspected to be ketotic and 30 healthy). Clinical and biochemical investigations were carried out for each buffalo. Based on clinical findings and the level of beta-hydroxybutyrate (BHB), buffalo were allocated into ketotic (42), subclinical cases (19). Clinically, there was an association between clinical ketosis and anorexia (p<0.001), constipation (p<0.001), decreased milk yield (p<0.001), ruminal stasis (p<0.001), and loss of body condition (p<0.01). Biochemically, in clinical ketosis compared with subclinical and control cases, there was a significant increase (p<0.05) of BHB, malondialdehyde (MDA), nitric oxide (NO), aspartate aminotransferase (AST), L-alanine aminotransferase (ALT). However, there was a significant decrease of glucose, phosphorus, magnesium,total cholesterol and HDL-cholesterol. There was a positive correlation between BHB and MDA (r=0.433), BHB and NO (r=0.37), MDA and NO (r=0.515), and Glucose and phosphorus(r=0.521). However, there was a negative correlation between BHB and glucose (r= -0.341) and HDL and NO (r= -0.379). The result of the present study indicates that hyperketonemia in buffalo is associated with an increase of oxidative stress levels. Further studies need to be done on the efficacy of antioxidants as an ancillary treatment to relief the oxidative stress caused by ketosis.

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Section: Discussionsupporting

confidence: 80%

“…NO is one of the most widespread signaling molecules, which participates in virtually cellular function in the body (Abou-Zeina et al 2009). When the influence of ketone bodies on spontaneous NO production was examined, a slight enhancement of NO production was detected (ZdzisinÂska et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level

Youssef

El-Khodery

El-Deeb

et al. 2010

Trop Anim Health Prod

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“…The pathophysiological mechanisms underlying increased NO production during diabetic ketosis have not been explored in this study: beside a low blood pH, increased concentrations of ketone bodies may trigger inappropriate NO production, as shown in endothelial cells from bovine aorta [26]. The correlation between the concentration of ketone bodies and plasma nitrates supports the hypothesis that these substances may be implicated in increasing NO production and may be the mechanism underlying the increase in iNOS expression and protein seen in lymphomonocytes.…”

Section: Discussionmentioning

confidence: 62%

“…It is interesting that, as hypothesized by Zdzisinska et al ., enhancement of NO synthesis in the presence of elevated ketone body concentrations can be considered a mechanism causing a predisposition to microbial infections [26]. Clearly inactivation of NO takes place in the erythrocytes and is brought about by the incorporation of molecular oxygen from HbO 2 [27].…”

Section: Discussionmentioning

confidence: 99%

Diabetic ketosis activates lymphomonocyte‐inducible nitric oxide synthase

et al. 2002

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“…2004). These very low glucose and high BHB concentrations are typically associated with clinical ketosis (Zdzisinska et al. 2000).…”

Section: Methodsmentioning

confidence: 99%

The In Vitro Development of Bovine Oocytes after Maturation in Glucose and β‐Hydroxybutyrate Concentrations Associated with Negative Energy Balance in Dairy Cows

Leroy

Vanholder

Opsomer

et al. 2006

Reprod Domestic Animals

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Negative energy balance (NEB) in high yielding dairy cows early postpartum may affect oocyte quality. Therefore, we tested the effect of two different beta-hydroxybutyrate (BHB) and glucose concentrations, which are associated with subclinical or clinical ketosis, during in vitro maturation (IVM) on the developmental competence of bovine oocytes. In Expt 1, subclinical ketosis conditions were imitated. Oocytes were matured in four different serum-free media with two glucose concentrations (g1=2.75 mm or G1=5.5 mm glucose) and with or without BHB (BHB1=1.8 mm BHB). Following maturation groups were used: g1, G1, g1:BHB1 and G1:BHB1. In Expt 2, clinical ketosis conditions were mimicked by using the concentrations: g2=1.375 mm or G2=3.1 mm glucose and BHB2=4.0 mm BHB. The combinations used were: g2, G2, g2:BHB2 and G2:BHB2. After IVM and in vitro fertilization (IVF), presumptive zygotes were routinely cultured for 7 days in synthetic oviduct fluid [SOF; 5% fetal calf serum (FCS)]. At 48 h and 8 days pi, cleavage rate and number of blastocysts were recorded respectively. The results demonstrated that the maturation conditions mimicking subclinical (g1:BHB1) and clinical ketosis (g2 : BHB2) resulted in an impaired developmental competence of the oocyte after maturation. Especially the moderately low (g1) or extremely low glucose (g2) concentrations were responsible for this detrimental effect that was associated with a blocked cumulus expansion. Only in moderately low glucose conditions (g1:BHB1), BHB exerted an additive toxic effect during oocyte maturation resulting in a reduced blastocyst rate. Conclusively, our results may suggest that subclinical and clinical ketosis can affect the oocyte's developmental competence most likely through a directly adverse effect of the low glucose concentrations on oocyte maturation. Only in subclinical conditions this harmful effect may be aggravated by BHB.

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The influence of ketone bodies and glucose on interferon, tumor necrosis factor production and NO release in bovine aorta endothelial cells

Cited by 14 publications

References 20 publications

Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level

Ketosis in buffalo (Bubalus bubalis): clinical findings and the associated oxidative stress level

Diabetic ketosis activates lymphomonocyte‐inducible nitric oxide synthase

The In Vitro Development of Bovine Oocytes after Maturation in Glucose and β‐Hydroxybutyrate Concentrations Associated with Negative Energy Balance in Dairy Cows

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