The influence of hydroxyapatite content on properties of poly(L-lactide)/hydroxyapatite porous scaffolds obtained using thermal induced phase separation technique

Szustakiewicz, Konrad; Gazińska, Małgorzata; Kryszak, Bartłomiej; Grzymajło, Michał; Pigłowski, J.; Wiglusz, Rafał J.; Okamoto, Masami

doi:10.1016/j.eurpolymj.2019.01.073

Cited by 46 publications

(38 citation statements)

References 35 publications

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“…In this approach, the intended homogenous polymer solution underwents a phase separation under the proper temperature and separates into polymer-rich and polymer-lean regions. In the final step and after the solvent extraction step, the 3D porous scaffold is obtained 16 . In addition to the structural support, the bioactive molecules are also required for an effective and facilitated bone healing process.…”

Section: Introductionmentioning

confidence: 99%

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Samadian

Farzamfar

Vaez

et al. 2020

Sci Rep

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The focus of the current study was to develop a functional and bioactive scaffold through the combination of 3D polylactic acid (PLA)/polycaprolactone (PCL) with gelatin nanofibers (GNFs) and Taurine (Tau) for bone defect regeneration. GNFs were fabricated via electrospinning dispersed in PLA/PCL polymer solution, Tau with different concentrations was added, and the polymer solution converted into a 3D and porous scaffold via the thermally-induced phase separation technique. The characterization results showed that the scaffolds have interconnected pores with the porosity of up to 90%. Moreover, Tau increased the wettability and weight loss rate, while compromised the compressive strengths. The scaffolds were hemo- and cytocompatible and supported cell viability and proliferation. The in vivo studies showed that the defects treated with scaffolds filled with new bone. The computed tomography (CT) imaging and histopathological observation revealed that the PLA/PCL/Gel/Tau 10% provided the highest new bone formation, angiogenesis, and woven bone among the treatment groups. Our finding illustrated that the fabricated scaffold was able to regenerate bone within the defect and can be considered as the effective scaffold for bone tissue engineering application.

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Section: Introductionmentioning

confidence: 99%

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

Samadian

Farzamfar

Vaez

et al. 2020

Sci Rep

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“…Composites and nanocomposites based on bioresorbable thermoplastic aliphatic polyesters, i.e., poly(L-lactide) (PLLA), polycaprolactone (PCL) or copolymers thereof (like PGLA) and calcium hydroxyapatites have been widely studied due to their potential applications in regenerative medicine, especially for bone tissue engineering [12,20,21,22,23,24,25,26,27]. Usually, these systems can be used as implants for small bone defects or as scaffolds [28,29].…”

Section: Introductionmentioning

confidence: 99%

The Comprehensive Approach to Preparation and Investigation of the Eu3+ Doped Hydroxyapatite/poly(L-lactide) Nanocomposites: Promising Materials for Theranostics Application

et al. 2019

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In response to the need for new materials for theranostics application, the structural and spectroscopic properties of composites designed for medical applications, received in the melt mixing process, were evaluated. A composite based on medical grade poly(L-lactide) (PLLA) and calcium hydroxyapatite (HAp) doped with Eu3+ ions was obtained by using a twin screw extruder. Pure calcium Hap, as well as the one doped with Eu3+ ions, was prepared using the precipitation method and then used as a filler. XRPD (X-ray Powder Diffraction) and IR (Infrared) spectroscopy were applied to investigate the structural properties of the obtained materials. DSC (Differential Scanning Calorimetry) was used to assess the Eu3+ ion content on phase transitions in PLLA. The tensile properties were also investigated. The excitation, emission spectra as well as decay time were measured to determine the spectroscopic properties. The simplified Judd–Ofelt (J-O) theory was applied and a detailed analysis in connection with the observed structural and spectroscopic measurements was made and described.

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“…However, all these values are within the measurement error. The results show that the materials obtained in our research display a much higher stiffness than the corresponding materials with nano-hydroxyapatite [ 19 ]. The compressive strength of all the samples is in the range of 20.2–29.6 kPa (at 40% strain) and 147.4–190.0 kPa (at 80% strain).…”

Section: Resultsmentioning

confidence: 99%

“…Bioresorbable polymer composites can be prepared in several ways, including melt mixing–extrusion [ 13 , 15 ] and solvent techniques: solution casting [ 16 ] electrospinning [ 17 , 18 ], and thermally induced phase separation (TIPS) [ 18 , 19 , 20 ]. The last of these techniques enables one to prepare composites in the form of a foam, which can be used as the so-called scaffold that stimulates bone cells to grow on its surface [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Pore Size Distribution and l-Lysine Modified Apatite Whiskers (HAP) on Osteoblasts Response in PLLA/HAP Foam Scaffolds Obtained in the Thermally Induced Phase Separation Process

Szustakiewicz

Włodarczyk

Gazińska

et al. 2021

IJMS

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In this research, we prepared foam scaffolds based on poly(l-lactide) (PLLA) and apatite whiskers (HAP) using thermally induced phase separation technique supported by the salt leaching process (TIPS-SL). Using sodium chloride having a size of (a) 150–315 μm, (b) 315–400 μm, and (c) 500–600 μm, three types of foams with different pore sizes have been obtained. Internal structure of the obtained materials has been investigated using SEM as well as μCT. The materials have been studied by means of porosity, density, and compression tests. As the most promising, the composite prepared with salt size of 500–600 μm was prepared also with the l-lysine modified apatite. The osteoblast hFOB 1.19 cell response for the scaffolds was also investigated by means of cell viability, proliferation, adhesion/penetration, and biomineralization. Direct contact cytotoxicity assay showed the cytocompatibility of the scaffolds. All types of foam scaffolds containing HAP whiskers, regardless the pore size or l-lysine modification induced significant stimulatory effect on the cal-cium deposits formation in osteoblasts. The PLLA/HAP scaffolds modified with l-lysine stimulated hFOB 1.19 osteoblasts proliferation. Compared to the scaffolds with smaller pores (150–315 µm and 315–400 µm), the PLLA/HAP foams with large pores (500–600 µm) promoted more effective ad-hesion of osteoblasts to the surface of the biomaterial.

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The influence of hydroxyapatite content on properties of poly(L-lactide)/hydroxyapatite porous scaffolds obtained using thermal induced phase separation technique

Cited by 46 publications

References 35 publications

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

The Comprehensive Approach to Preparation and Investigation of the Eu3+ Doped Hydroxyapatite/poly(L-lactide) Nanocomposites: Promising Materials for Theranostics Application

The Effect of Pore Size Distribution and l-Lysine Modified Apatite Whiskers (HAP) on Osteoblasts Response in PLLA/HAP Foam Scaffolds Obtained in the Thermally Induced Phase Separation Process

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